炭菌联作对盐碱土性质及甜瓜光合指标的影响

张雅婷; 楼雪怡; 吴嘉睿; 刘宏缘; 叶玢妤; 肖佳敏; 李佳萌; 缪怡宁; 陈馨妍; 刘怡琳; 谭驭宇; 王志超; 刘鹏

doi:10.13870/j.cnki.stbcxb.2024.06.039

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炭菌联作对盐碱土性质及甜瓜光合指标的影响

更新时间：2025-02-18

- 炭菌联作对盐碱土性质及甜瓜光合指标的影响
- Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon
- 水土保持学报 2024年38卷第6期页码：352-362
- 作者机构：
  
  浙江师范大学生命科学学院植物学实验室,浙江,金华,321004
- 作者简介：
  
  [ "张雅婷(2003—),女,硕士研究生,主要从事环境生物技术研究。E-mail:zyt5132384@163.com" ]
- 基金信息：
  
  国家自然科学基金项目(41571049,41461010);2023年浙江省大学生创新创业训练计划项目(S202310345048)
- DOI：10.13870/j.cnki.stbcxb.2024.06.039
  中图分类号： S154.39
- 纸质出版：2024
- 稿件说明：
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张雅婷, 楼雪怡, 吴嘉睿, 等. 炭菌联作对盐碱土性质及甜瓜光合指标的影响[J]. 水土保持学报, 2024,38(6):352-362.

ZHANG Yating, LOU Xueyi, WU Jiarui, et al. Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon[J]. 2024, 38(6): 352-362.
张雅婷, 楼雪怡, 吴嘉睿, 等. 炭菌联作对盐碱土性质及甜瓜光合指标的影响[J]. 水土保持学报, 2024,38(6):352-362. DOI： 10.13870/j.cnki.stbcxb.2024.06.039.

ZHANG Yating, LOU Xueyi, WU Jiarui, et al. Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon[J]. 2024, 38(6): 352-362. DOI： 10.13870/j.cnki.stbcxb.2024.06.039.

摘要

[目的] 为改良腐殖酸—磁性生物炭(HA—MBC)

并探究改良后的HA—MBC与丛枝菌根真菌(AMF)联合作用对盐碱土性质与甜瓜生长的影响。[方法] 通过响应面优化法

分析磁性生物炭与腐殖酸配比(1∶4

1∶6

1∶8)、FeCl3、FeSO4试剂浓度(0.02

0.04

0.06 g/mL)及交联时长(48

96 h)对HA—MBC的影响

获取适合与AMF联施的最优改良方案。并通过盆栽试验

探究单菌、单炭和炭菌联作(AMF+10%B、AMF+20%B)对盐土、碱土理化性质

酶活性和甜瓜生长、光合指标的影响。[结果] 15

75 mmol/L盐碱胁迫时

20 g/kg AMF与20%HA—MBC共同施用缓解效果最优

二者"协同增效"。一方面

HA—MBC负载的铁氧化合物及HA通过增加表面官能团及孔隙率

改善土壤理化性质

而AMF能通过形成共生菌根

二次富集有机质、营养元素

并提升土壤酶活性

进而增加甜瓜株高根长

促进Fv/Fm的回升与F0、qN的降低;另一方面

HA—MBC的施用能够使盐土、碱土中AMF侵染率分别提高19.46%

22.72%

进一步提高AMF作用效果。同时

Fe2+、Mg2+、N等营养元素在炭菌间能形成转化循环

提高养分利用率。150 mmol/L盐碱胁迫时

施加低剂量HA—MBC仍起改良土壤、缓解胁迫的作用

而高剂量炭施加则对指标的改善起抑制作用。可能是过施HA—MBC时

表面官能团过度聚集有毒物质、盐离子并竞争营养元素

使AMF侵染率降低

削弱共生菌根现象

进而降低甜瓜光合指标。[结论] 在添加0.04 mmol/L FeCl3、FeSO4试剂

生物炭与腐殖酸配比6∶1

交联时间72 h下制备的HA—MBC适合与AMF共同施用。二者联作在低、中浓度盐碱胁迫水平下可改善盐碱土理化性质和养分结构

促进甜瓜光合作用

而高浓度胁迫下过施HA—MBC抑制土壤指标和甜瓜光合指标的回升。

Abstract

[Objective] To improve humic acid-modified magnetic biochar (HA-MBC) and explore the combined effects of modified biochar and arbuscular mycorrhizal fungi (AMF) on the properties of saline-alkali soils and the growth of muskmelon. [Methods] Through response surface methodology

the effects of the ratio of magnetic biochar to humic acid (1∶4

1∶6

1∶8)

the concentration of FeCl3 and FeSO4 reagents (0.02

0.04

0.06 g/mL)

and the cross-linking duration (48

96 h) on HA-MBC were analyzed

to procure the optimum amendment scheme suitable for combined application with AMF. Moreover

by conducting pot experiments

the influence of individual fungi

individual biochar

and combined biochar-fungi (AMF+10%B

AMF+20%B) on the physicochemical properties

and enzyme activities of saline soil and alkaline soil

and the photosynthetic parameters of muskmelon were studied. [Results] Under 15 and 75 mmol/L saline-alkali stress

combined application of 20 g/kg AMF with 20% HA-MBC showed the best mitigation effects

with a "synergistic enhancement" observed. On one hand

the iron oxides and humic acids loaded on HA-MBC improved the physical and chemical properties of soil by increasing the quantity of surface functional groups and porosity

while AMF formed symbiotic roots to further enrich organic matter and nutrients

and increase soil enzyme activities

thus increasing the height and root length of muskmelon and promoting the recovery of Fv/Fm and the reduction of F0 and qN. On the other hand

the application of HA-MBC could increase the colonization rate of AMF in saline and alkaline soils by 19.46% and 22.72%

respectively

thus further enhancing the effects of AMF. Moreover

nutrients such as Fe2+

Mg2+ and nitrogen could form a transformation cycle between biochar and fungi

thus improving nutrient use efficiency. Under 150 mmol/L saline-alkali stress

applying a low dose of HA-MBC still played a role in ameliorating soil and mitigating stress

while applying high doses of biochar inhibited the improvement of indicators. This might be due to excessive application of HA-MBC causing surface functional groups to aggregate toxic substances and salt ions excessively and compete for nutrients

subsequently reducing AMF colonization rates

weakening the mycorrhizal symbiosis

and hence diminishing the photosynthetic parameters of muskmelon. [Conclusion] HA-MBC prepared with a biochar to humic acid ratio of 6∶1 and cross-linking time of 72 h

using 0.04 mmol/L FeCl3 and FeSO4 reagents

is suitable for combined application with AMF. The synergistic application of HA-MBC and AMF can improve the physicochemical properties and nutrient structure of saline-alkali soil and promote the photosynthesis of muskmelon under low to medium saline-alkali stress levels

whereas excessive application of HA-MBC under high stress concentrations inhibits the improvement of soil indicators and the recovery of photosynthetic parameters in muskmelon.

关键词

Keywords

references

ABD EL-HALIM A E H A, SALAMA A M, IBRAHIM M M, et al. Nano-gypsum in low dose improves the physicochemical properties of saline-sodic soil[J].Archives of Agronomy and Soil Science,2023,69(12):2286-2299.

ZHAO Z R, LIU L, SUN Y, et al. Combined microbe-plant remediation of cadmium in saline-alkali soil assisted by fungal mycelium-derived biochar[J].Environmental Research,2024,240(Pt 2):e117424.

王则玉,马兴旺,李杨,等.不同酸化生物炭用量对滴灌棉田盐碱土水盐运移的影响[J].灌溉排水学报,2024,43(3):33-38. WANG Z Y, MA X W, LI Y, et al. Effects of acidified biochar amendment on water and salt movement in saline-alkali soil in drip-irrigated cotton field[J].Journal of Irrigation and Drainage,2024,43(3):33-38.

罗春峰,张晓蓉,巩宗强,等.活化铁尾砂与镁改性生物炭配施对水稻幼苗生长及盐碱土性质的影响[J].农业环境科学学报,2024,43(1):68-78. LUO C F, ZHANG X R, GONG Z Q, et al. Effect of activated iron tailings and magnesium-modified biochar blending on rice seedling growth and saline-alkali soil properties[J].Journal of Agricultural and Environmental Science,2024,43(1):68-78.

吴增学,刘晶静,马文丹.生物炭和丛枝菌根真菌配施强化重金属污染土壤植物修复研究进展[J].环境污染与防治,2024,46(7):1040-1046. WU Z X, LIU J J, MA W D. Research progress on heavy metal contaminated soil phytoremediation enhanced by combining biochar and arbuscular mycorrhizal fungi[J].Environmental Pollution and Control,2024,46(7):1040-1046.

王翔宇.生物炭与丛枝菌根真菌共同改良滨海盐碱土促进植物生长的研究[D].山东青岛:中国海洋大学,2019. WANG X Y. Promotion of plant growth of biochar together with arbuscular mycorrhizal fungi[D].Qingdao, Shandong: Ocean University of China,2019.

马啸,熊巧,周香君,等.赤铁矿改性生物炭回收水中磷及其作为磷肥的效果[J].农业工程学报,2023,39(22):216-225. MA X, XIONG Q, ZHOU X J, et al. Phosphate reclamation from water using biochar modified with hematite and its application as soil phosphorus fertilizer[J].Transactions of the Chinese Society of Agricultural Engineering,2023,39(22):216-225.

王欢.腐殖酸—磁性生物炭对轻度盐碱地修复及植物生长特性的影响机理研究[D].上海:东华大学,2022. WANG H. Mechanism of humic acid-magnetic biochar on mild salinity-alkali field remediation and plant growth characteristics[D]. Shanghai: Donghua University,2022.

HAGEMANN N, SUBDIAGA E, ORSETTI S, et al. Effect of biochar amendment on compost organic matter composition following aerobic composting of manure[J].The Science of the Total Environment,2018,613/614:20-29.

CHEN W L, SHAN W S, NIU T T, et al. Insight into regulation of adventitious root formation by arbuscular mycorrhizal fungus and exogenous auxin in tea plant (

Camellia sinensis

L.) cuttings[J].Frontiers in Plant Science,2023,14:e1258410.

栾慧,刘红,高一娜,等.土壤速效氮测定方法的改进[J].湖南农业科学,2017(3):88-89. LUAN H, LIU H, GAO Y N, et al. Study on the determination method of soil alkali hydrolysis nitrogen[J].Hunan Agricultural Sciences,2017(3):88-89.

HOSSAIN M Z, BAHAR M M, SARKAR B, et al. Biochar and its importance on nutrient dynamics in soil and plant[J].Biochar,2020,2(4):379-420.

BAZARGAN K, MARZI M, HASHEMINASAB K S, et al. Optimizing ammonium acetate procedure for determining available potassium in Iranian calcareous soils, testing the concentration and contact time[J].Journal of Soil Science Society of Iran,2022,1(1):125-136.

王传杰,王齐齐,徐虎,等.长期施肥下农田土壤-有机质-微生物的碳氮磷化学计量学特征[J].生态学报,2018,38(11):3848-3858. WANG C J, WANG Q Q, XU H, et al. Carbon, nitrogen, and phosphorus stoichiometry characteristics of bulk soil, organic matter, and soil microbial biomass under long-term fertilization in cropland[J].Acta Ecologica Sinica,2018,38(11):3848-3858.

LI F, CHOONG T S Y, SOLTANI S, et al. Investigation of glyphosate removal from aqueous solutions using fenton-like system based on calcium peroxide[J].Processes,2022,10(10):e2045.

LU Q, LI C L, WU G S. Insight into the inhibitory effects of

Zanthoxylum nitidum

against

Helicobacter pylori urease

and jack bean urease: Kinetics and mechanism[J].Journal of Ethnopharmacology,2020,249:e112419.

陈佳琦,吴海燕,姚琳,等.基于溶胶-凝胶包埋的蛋白磷酸酶抑制比色法快速检测腹泻性贝类毒素[J].分析化学,2018,46(8):1261-1268. CHEN J Q, WU H Y, YAO L, et al. Rapid determination of diarrhetic shellfish poisoning in shellfish by colorimetric protein phosphatase inhibition assay based on immobilization of protein phosphatase 2A in sol-gel[J].Chinese Journal of Analytical Chemistry,2018,46(8):1261-1268.

LU H S, LIU Y Q, LIU W J, et al. Isolation, identification of Chlorella pyrenoides from aquaculture wastewater and its purification of wastewater[J].Editorial Office of Transactions of the Chinese Society of Agricultural Engineering,2017,33(4):273-277.

韦莉莉,卢昌熠,丁晶,等.丛枝菌根真菌参与下植物-土壤系统的养分交流及调控[J].生态学报,2016,36(14):4233-4243. WEI L L, LU C Y, DING J, et al. Functional relationships between arbuscular mycorrhizal symbionts and nutrient dynamics in plant-soil-microbe system[J].Acta Ecologica Sinica,2016,36(14):4233-4243.

王红萍,步连燕,陈文峰,等.功能菌剂与生物炭配施对沙化土壤的影响[J].水土保持学报,2023,37(1):345-353. WANG H P, BU L Y, CHEN W F, et al. Effects of combined application of functional microbial agents and biochar on sandy soil[J].Journal of Soil and Water Conservation,2023,37(1):345-353.

江美琳,金辉,邓聪,等.生物炭负载Fe

纳米粒子的制备与表征[J].农业环境科学学报,2018,37(3):592-597. JIANG M L, JIN H, DENG C, et al. Preparation and characterization of nanoparticles containing Fe

cores in biochar[J].Journal of Agro-Environment Science,2018,37(3):592-597.

YANG F, DU Q, SUI L, et al. One-step fabrication of artificial humic acid-functionalized colloid-like magnetic biochar for rapid heavy metal removal[J].Bioresource Technology,2021,328:e124825.

曾凤美,孙思佳,孙恩惠,等. Fe/La定向修饰凹凸棒土稻壳基颗粒成型生物炭:磷酸盐吸附行为与机制[J].环境科学学报,2022,42(7):279-289. ZENG F M, SUN S J, SUN E H, et al. Fe/La directed modification of rice hull-based particles to form biochar: Phosphate adsorption behavior and mechanism[J].Journal of Environmental Science,2022,42(7):279-289.

赵维彬,王松,刘玲玲,等.生物炭改良盐碱地效果及其对植物生长的影响研究进展[J].土壤通报,2024,55(2):551-561. ZHAO W B, WANG S, LIU L L, et al. Effect of biochar amendment on saline-alkaline soil amelioration and plant growth: A literature review[J].Chinese Journal of Soil Science,2024,55(2):551-561.

王岩,周鹏,白立伟,等.生物炭和AM真菌配施对连作辣椒生长和土壤养分的影响[J].中国生态农业学报(中英文),2020,28(10):1600-1608. WANG Y, ZHOU P, BAI L W, et al. Effects of biochar and arbuscular mycorrhizal fungi on the growth of continuous cropping pepper and soil nutrient status[J].Chinese Journal of Eco-Agriculture,2020,28(10):1600-1608.

WU S L, FU W, RILLIG M C, et al. Soil organic matter dynamics mediated by arbuscular mycorrhizal fungi-an updated conceptual framework[J].The New Phytologist,2024,242(4):1417-1425.

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