滇中高原云南松林土壤微生物群落结构及其多样性对氮沉降的响应

宋成功; 王克勤; 宋娅丽; 李晓东; 张继国; 李芸; 邓秋江

doi:10.13870/j.cnki.stbcxb.2024.06.009

您当前的位置：

首页 >

文章列表页 >

滇中高原云南松林土壤微生物群落结构及其多样性对氮沉降的响应

更新时间：2025-02-18

- 滇中高原云南松林土壤微生物群落结构及其多样性对氮沉降的响应
- Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of
- 水土保持学报 2024年38卷第6期页码：273-283
- 作者机构：
  
  1. 西南林业大学生态与环境学院,昆明,650224
  2. 国家林业和草原局云南玉溪森林生态系统国家定位观测研究站,云南,玉溪,653100
  3. 禄劝彝族苗族自治县林业和草原局, 云南禄劝,651500
- 作者简介：
  
  [ "宋成功(1999—),男,硕士,主要从事森林生态研究。E-mail:scg19990505@sina.com" ]
- 基金信息：
  
  云南省农业基础研究联合专项(202301BD070001-059);云南省教育厅科学研究基金项目(2022J0510,2023Y0717);国家自然科学基金地区项目(31960318);云南省自然生态监测网络项目云南玉溪森林生态站运行项目(2022-YN-13);云南玉溪森林生态系统国家长期科研基地项目(2020132550);云南省一流学科建设项目(云教发〔2022〕73号)
- DOI：10.13870/j.cnki.stbcxb.2024.06.009
  中图分类号： S718.54
- 纸质出版：2024
- 稿件说明：
移动端阅览
宋成功, 王克勤, 宋娅丽, 等. 滇中高原云南松林土壤微生物群落结构及其多样性对氮沉降的响应[J]. 水土保持学报, 2024,38(6):273-283.

SONG Chenggong, WANG Keqin, SONG Yali, et al. Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of[J]. 2024, 38(6): 273-283.
宋成功, 王克勤, 宋娅丽, 等. 滇中高原云南松林土壤微生物群落结构及其多样性对氮沉降的响应[J]. 水土保持学报, 2024,38(6):273-283. DOI： 10.13870/j.cnki.stbcxb.2024.06.009.

SONG Chenggong, WANG Keqin, SONG Yali, et al. Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of[J]. 2024, 38(6): 273-283. DOI： 10.13870/j.cnki.stbcxb.2024.06.009.

摘要

[目的] 探究氮沉降下滇中高原云南松土壤化学性质及土壤微生物群落多样性和结构组成的变化特征

并分析土壤微生物与土壤化学性质的相关关系

为了解氮沉降下森林土壤生态系统养分循环变化趋势及观测土壤微生态变化提供科学依据。[方法] 选取滇中高原云南松林土壤为研究对象

以CO(NH2)2为N源

设置对照CK [N 0 g/(m2·a)]、低氮LN [N 10 g/(m2·a)]、中氮MN [N 20 g/(m2·a)]和高氮HN [N 25 g/(m2·a)]4个不同梯度进行模拟N沉降试验

测定分析土壤细菌、真菌群落结构及多样性和土壤pH、有机碳(TOC)、全氮(TN)、全磷(TP)、铵态氮(NH+4-N)和硝态氮(NO-3-N)的变化特征。[结果] (1)在不同季节下

与CK相比各氮处理下土壤pH、TOC含量均降低

TN均升高

NH+4-N和NO-3-N均在MN、HN处升高;(2)细菌和真菌α多样性雨季均高于旱季;与CK相较细菌α多样性在2个季节下LN处上升

而真菌α多样性则在旱季LN处上升;(3)在旱季和雨季细菌优势菌门均为变形菌门、酸杆菌门和放线菌门

真菌优势菌门为担子菌门、子囊菌门和被孢菌门;(4)在旱季和雨季

细菌和真菌优势菌属与pH、TOC、TN、NO-3-N呈显著相关关系。[结论] 氮沉降可以为土壤微生物提供NH+4-N和NO-3-N等有效养分

但由于氮沉降导致土壤酸化使土壤TOC含量降低

影响土壤微生物群落结构。

Abstract

[Objective] To study the characteristics of changes in chemical properties and diversity and structural composition of soil microbial communities in Pinus yun-nanensis soils on the Central Yunnan Plateau under nitrogen deposition

and to analyze the correlation between soil microorganisms and soil chemical properties

in order to provide a scientific basis for understanding the trend of changes in nutrient cycling in forest soil ecosystems under nitrogen deposition and observing soil microbiological changes. [Method] The soil of P. yunnanensis forests on the Central Yunnan Plateau was selected as the research object

CO(NH2)2 was used as the N source

and four different gradients were set up for simulated N deposition experiments

including control CK [N 0 g/(m2·a)]

low nitrogen LN [N 10 g/(m2·a)]

medium nitrogen MN [N 20 g/(m2·a)]

and high nitrogen HN [N 25 g/(m2·a)]

the structure and diversity of soil bacterial and fungal community were analyzed

and the characteristics of the changes of soil pH

TOC

NH+4-N and NO-3-N were determined. [Results] (1) In both the dry and rainy seasons

soil pH and TOC content were reduced

and TN was increased under each N treatment when compared with CK

and both NH+4-N and NO-3-N were increased at MN and HN compared to CK; (2) Both bacterial and fungal α-diversity were higher in the rainy season than in the dry season; bacterial α-diversity increased at LN under both seasons compared to CK

whereas fungi α-diversity increased at LN in the dry season; (3) In both the dry and rainy seasons

the dominant bacterial phyla were Proteobacteria

Acidobacteria and Actinobacteria

and the dominant fungal phyla were Basidiomycota

Ascomycota and Mortierellomycota; (4) The dominant bacterial and fungal genera were significantly correlated with pH

TOC

TN and NO-3-N during the dry and wet seasons. [Conclusion] Nitrogen deposition can provide soil microorganisms with effective nutrients such as NH+4-N and NO-3-N

but soil acidification due to nitrogen deposition can reduce soil TOC content and affect the structure of soil microbial communities.

关键词

Keywords

references

ZUCCARINI P, ASENSIO D, SARDANS J, et al. Effects of nitrogen deposition on soil enzymatic activity and soil microbial community in a Mediterranean holm oak forest[J].Geoderma,2023,430:e116354.

黄幸然,郭萍萍,吴旺旺,等.模拟氮沉降增加对不同树种土壤微生物群落结构的影响[J].生态学杂志,2016,35(6):1420-1426. HUANG X R, GUO P P, WU W W, et al. Influence of simulated nitrogen deposition enhancement on soil microbial community of different tree species[J].Chinese Journal of Ecology,2016,35(6):1420-1426.

刘春华,吴东梅,刘雨晖,等.氮沉降对米槠天然林土壤有机碳及微生物群落结构的影响[J].林业科学研究,2021,34(2):42-49. LIU C H, WU D M, LIU Y H, et al. Effects of nitrogen deposition on soil organic carbon and soil microbial communities in a natural

Castanopsis carlesii

forest[J].Forest Research,2021,34(2):42-49.

LI Y B, BEZEMER T M, YANG J J, et al. Changes in litter quality induced by N deposition alter soil microbial communities[J].Soil Biology and Biochemistry,2019,130:33-42.

隋心,张荣涛,杨立宾,等.模拟氮沉降对三江平原小叶章湿地土壤细菌多样性的影响[J].草业科学,2016,33(4):589-598. SUI X, ZHANG R T, YANG L B, et al. Effect of simulation nitrogen depositions on bacterial diversity of

Deyeuxia angustifolia

in wetland of Sanjiang Plain[J].Pratacultural Science,2016,33(4):589-598.

HU Z K, DELGADO-BAQUERIZO M, FANIN N, et al. Nutrient-induced acidification modulates soil biodiversity-function relationships[J].Nature Communications,2024,15(1):e2858.

YANG A, SONG B, ZHANG W X, et al. Chronic enhanced nitrogen deposition and elevated precipitation jointly benefit soil microbial community in a temperate forest[J].Soil Biology and Biochemistry,2024,193:e109397.

SHI L L, ZHANG H Z, LIU T, et al. An increase in precipitation exacerbates negative effects of nitrogen deposition on soil cations and soil microbial communities in a temperate forest[J].Environmental Pollution,2018,235:293-301.

郝亚群,谢麟,陈岳民,等.中亚热带地区氮沉降对杉木幼林土壤细菌群落多样性及组成的影响[J].应用生态学报,2018,29(1):53-58. HAO Y Q, XIE L, CHEN Y M, et al. Effects of nitrogen deposition on diversity and composition of soil bacterial community in a subtropical

Cunninghamia lanceolata

plantation[J].Chinese Journal of Applied Ecology,2018,29(1):53-58.

郑裕雄,曹际玲,杨智杰,等.氮沉降对亚热带常绿阔叶天然林不同季节土壤微生物群落结构的影响[J].土壤学报,2018,55(6):1534-1544. ZHENG Y X, CAO J L, YANG Z J, et al. Impacts of nitrogen deposition on soil microbial community structure in subtropical natural evergreen broad-leaved forest relative to season[J].Acta Pedologica Sinica,2018,55(6):1534-1544.

张乃木,王克勤,宋娅丽,等.施N对云南松凋落叶和枝早期分解过程中养分动态变化的影响[J].中南林业科技大学学报,2023,43(3):135-144. ZHANG N M, WANG K Q, SONG Y L, et al. Effects of N application on nutrient dynamics in

Pinus yunnanensis

forest during the early decomposition of leaf and twig litter[J].Journal of Central South University of Forestry and Technology,2023,43(3):135-144.

张英,张常洪,汪其同,等.氮沉降下西南山地针叶林根际和非根际土壤微生物养分限制特征差异[J].植物生态学报,2022,46(4):473-483. ZHANG Y, ZHANG C H, WANG Q T, et al. Difference of microbial nutrient limiting characteristics in rhizosphere and bulk soil of coniferous forests under nitrogen deposition in southwest mountain, China[J].Chinese Journal of Plant Ecology,2022,46(4):473-483.

胡峻嶍,陈蕙心,周世兴,等.模拟氮沉降对华西雨屏区天然常绿阔叶林凋落叶分解过程中K、Ca、Mg元素释放的影响[J].林业科学研究,2020,33(3):124-131. HU J X, CHEN H X, ZHOU S X, et al. Effects of simulated nitrogen deposition on the releases of potassium, calcium, and magnesium during litter decomposition in a natural evergreen broadleaved forest in the rainy area of Western China[J].Forest Research,2020,33(3):124-131.

周慧娴,沈傲,崔寅平,等.区域氮干沉降分布特征的数值模拟[J].环境科学学报,2022,42(10):129-137. ZHOU H X, SHEN A, CUI Y P, et al. Numerical simulation of distribution characteristics of regional nitrogen dry deposition[J].Acta Scientiae Circumstantiae,2022,42(10):129-137.

肖春艳,胡情情,陈晓舒,等.基于文献计量的大气氮沉降研究进展[J].生态学报,2023,43(3):1294-1307. XIAO C Y, HU Q Q, CHEN X S, et al. Research progress of atmospheric nitrogen deposition based on bibliometrics[J].Acta Ecologica Sinica,2023,43(3):1294-1307.

鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000. BAO S D. Soil and Agricultural Chemistry Analysis[M].3rd ed. Beijing: China Agriculture Press,2000.

高浩诚,牧仁,焦婷,等.施氮对高寒垂穗披碱草草地根际、非根际土壤肥力的影响[J].水土保持学报,2023,37(6):276-282. GAO H C, MU R, JIAO T, et al. Effects of nitrogen application on rhizosphere and non-rhizosphere soil fertility of alpine

Elymus nutans

grassland[J].Journal of Soil and Water Conservation,2023,37(6):276-282.

张雪,梅莉,宋利豪,等.模拟氮沉降对马尾松土壤微生物群落结构及温室气体释放的影响[J].生态学报,2019,39(6):1917-1925. ZHANG X, MEI L, SONG L H, et al. Effects of simulated nitrogen deposition on microbial community and greenhouse gases emission of

Pinus massoniana

soil[J].Acta Ecologica Sinica,2019,39(6):1917-1925.

简俊楠,刘伟超,朱玉帆,等.短期氮添加对黄土高原人工刺槐林土壤有机碳组分的影响[J].环境科学,2023,44(5):2767-2774. JIAN J N, LIU W C, ZHU Y F, et al. Effects of short-term nitrogen addition on soil organic carbon components in

Robinia pseudoacacia

L. plantation[J].Environmental Science,2023,44(5):2767-2774.

刘永万,白炜,尹鹏松,等.外源氮素添加对长江源区高寒沼泽草甸土壤养分及植物群落生物量的影响[J].草地学报,2020,28(2):483-491. LIU Y W, BAI W, YIN P S, et al. Effects of exogenous nitrogen addition on soil nutrients and plant community biomass in alpine swamp meadow in the headwaters region of the Yangtze River[J].Acta Agrestia Sinica,2020,28(2):483-491.

卢朝阳,马维伟,杜佳囝,等.尕海湿地区沼泽草甸生长季土壤碳氮组分对增温施氮的响应[J].水土保持学报,2024,38(2):316-325. LU Z Y, MA W W, DU J N, et al. Response of growing season soil carbon and nitrogen components unity composition and function characteristics of different plantations of sandy area, northwest Liaoning Province[J].Forest Research,2020,33(1):44吭阵栴.杨雪岩,林媛媛,等.刺槐和油松枯落叶腐殖质化对气候温湿变化的响应[J].草地学报,2021,29(8):1738-1747. WU X M, YANG X Y, LIN Y Y, et al. Response of humification of

Robinia pseudoacacia

and

Pinus tabulaeformis

litter to climate temperature and humidity changes[J].Acta Agrestia Sinica,2021,29(8):1738-1747.

吕晶花,赵旭燕,陆梅,等.氮沉降下纳帕海草甸植被与土壤变化对微生物生物量碳氮的影响[J].应用生态学报,2023,34(6):1525-1532. LYU J H, ZHAO X Y, LU M, et al. Effects of vegetation and soil changes on microbial biomass carbon and nitrogen in the Napahai meadow under N deposition[J].Chinese Journal of Applied Ecology,2023,34(6):1525-1532.

CAO M M, ZHENG X, CUI L N, et al. Soil bacterial communities are more sensitive to short-term nitrogen deposition than fungal communities in subtropical Chinese fir forests[J].Forest Ecology and Management,2023,549:e121490.

聂江文,王幼娟,吴邦魁,等.施氮对冬种紫云英不还田条件下稻田土壤微生物数量与结构的影响[J].生态学杂志,2018,37(12):3617-3624. NIE J W, WANG Y J, WU B K, et al. Effects of nitrogen application on the abundance and community of soil microbes in paddy field under the condition of no returning Chinese milk vetch[J].Chinese Journal of Ecology,2018,37(12):3617-3624.

YANG Y, CHENG H, GAO H, et al. Response and driving factors of soil microbial diversity related to global nitrogen addition[J].Land Degradation and Development,2020,31(2):190-204.

杨立宾,隋心,崔福星,等.汤旺河国家公园不同演替阶段森林土壤细菌多样性变化规律[J].环境科学研究,2019,32(3):458-464. YANG L B, SUI X, CUI F X, et al. Soil bacterial diversity between different forest successional stages in Tangwanghe National Park[J].Research of Environmental Sciences,2019,32(3):458-464.

鲁连欣,王克勤,李珠宇,等.等高反坡台阶整地对不同耕作系统根土微生态的影响[J].水土保持学报,2024,38(2):326-338. LU L X, WANG K Q, LI Z Y, et al. Effects of contour reverse-slope terrace on the microecology of rhizosphere in different farming systems[J].Journal of Soil and Water Conservation,2024,38(2):326-338.

赵雯,王丹丹,热依拉·木民,等.阿尔山地区兴安落叶松林土壤微生物群落结构[J].生物多样性,2023,31(2):155-166. ZHAO W, WANG D D, REYILA M M, et al. Soil microbial community structure of

Larix gmelinii

forest in the Aershan area[J].Biodiversity Science,2023,31(2):155-166.

谢欢,张秋芳,曾泉鑫,等.氮添加对杉木苗期磷转化和分解类真菌的影响[J].植物生态学报,2022,46(2):220-231. XIE H, ZHANG Q F, ZENG Q X, et al. Effects of nitrogen addition on phosphorus transformation and decomposition fungi in seedling stage of

Cunninghamia lanceolata

.Chinese Journal of Plant Ecology,2022,46(2):220-231.

YANG Y, QIU K Y, XIE Y Z, et al. Geographical, climatic, and soil factors control the altitudinal pattern of rhizosphere microbial diversity and its driving effect on root zone soil multifunctionality in mountain ecosystems[J].Science of the Total Environment,2023,904:e166932.

肖文贤,宋娅丽,王克勤,等.氮添加对滇中高原森林凋落物养分残留及其持水性的影响[J].水土保持学报,2023,37(3):319-329. XIAO W X, SONG Y L, WANG K Q, et al. Influence of nitrogen addition on nutrient residues and water holding capacity of forest litter in central Yunnan Plateau, China[J].Journal of Soil and Water Conservation,2023,37(3):319-329.

HE D, XIANG X J, HE J S, et al. Composition of the soil fungal community is more sensitive to phosphorus than nitrogen addition in the alpine meadow on the Qinghai-Tibetan Plateau[J].Biology and Fertility of Soils,2016,52(8):1059-1072.

刘晶晶,李金花,季燕,等.辉河湿地河岸带土壤微生物群落组成与土壤理化关系[J].草地学报,2023,31(5):1393-1405. LIU J J, LI J H, JI Y, et al. Relationship between soil microbial community composition and soil physicochemical properties in riparian zone of Huihe wetland[J].Acta Agrestia Sinica,2023,31(5):1393-1405.

邓玉峰,田善义,成艳红,等.模拟氮沉降下施石灰对休耕红壤优势植物根际土壤微生物群落的影响[J].土壤学报,2019,56(6):1449-1458. DENG Y F, TIAN S Y, CHENG Y H, et al. Effects of liming on rhizosphere soil microbial communities of dominant plants in fallowed red soil under simulated nitrogen deposition[J].Acta Pedologica Sinica,2019,56(6):1449-1458.

邓娇娇,朱文旭,张岩,等.辽西北风沙区不同人工林土壤真菌群落结构及功能特征[J].林业科学研究,2020,33(1):44-54. DENG J J, ZHU W X, ZHANG Y, et al. Studies on soil fungal comm

浏览量

537

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

青藏高原草甸土微生物活性对不同冻融循环次数的非线性响应

黄芩渣有机肥对沙地连作马铃薯农田土壤细菌多样性与功能的影响

林分类型和氮添加对亚热带森林土壤氧化亚氮排放的影响

氮磷添加对常绿阔叶林土壤团聚体

放牧强度对荒漠草原土壤微生物群落特征的影响