外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响

赵娜; 李瑞东; 王小利; 段建军; 郑钦文; 李明瑞

doi:10.13870/j.cnki.stbcxb.2023.06.030

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外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响

更新时间：2025-03-12

- 外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响
- Effects of Exogenous Calcium Carbonate and Temperature on Active Organic Carbon Components and Microbial Mommunity Composition in Yellow Soil
- 水土保持学报 2023年37卷第6期页码：238-245
- 作者机构：
  
  1. 贵州大学农学院,贵阳,550025
  2. 贵州大学烟草学院, 贵州省烟草品质研究重点实验室,贵阳,550025
- 作者简介：
  
  [ "赵娜(1998-),女,在读硕士研究生,主要从事土壤有机碳稳定性研究。E-mail:3082054232@qq.com" ]
- 基金信息：
  
  国家自然科学基金项目(31860160)
- DOI：10.13870/j.cnki.stbcxb.2023.06.030
  中图分类号：
- 纸质出版：2023
- 稿件说明：
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赵娜, 李瑞东, 王小利, 等. 外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响[J]. 水土保持学报, 2023,37(6):238-245.

ZHAO Na, LI Ruidong, WANG Xiaoli, et al. Effects of Exogenous Calcium Carbonate and Temperature on Active Organic Carbon Components and Microbial Mommunity Composition in Yellow Soil[J]. 2023, 37(6): 238-245.
赵娜, 李瑞东, 王小利, 等. 外源碳酸钙和温度对黄壤活性有机碳组分及微生物群落组成的影响[J]. 水土保持学报, 2023,37(6):238-245. DOI： 10.13870/j.cnki.stbcxb.2023.06.030.

ZHAO Na, LI Ruidong, WANG Xiaoli, et al. Effects of Exogenous Calcium Carbonate and Temperature on Active Organic Carbon Components and Microbial Mommunity Composition in Yellow Soil[J]. 2023, 37(6): 238-245. DOI： 10.13870/j.cnki.stbcxb.2023.06.030.

摘要

为探究不同温度条件下添加碳酸钙对活性有机碳组分及微生物群落组成的影响

以贵州典型黄壤土为研究对象

采用同位素标记法

设置6个处理(培养温度分别为15

35 ℃下添加碳酸钙和不添加碳酸钙)

通过分析不同温度下碳酸钙添加对土壤活性有机碳、微生物群落组成的影响

揭示外源碳在土壤活性碳库中的分配规律

以期为贵州典型黄壤有机碳固存和改良提供理论依据。结果表明:各温度下添加碳酸钙显著增加土壤DOC和MBC含量(p＜0.01)

添加碳酸钙各处理土壤DOC含量在培养第5天均达到最大值

相较于不添加碳酸钙处理

添加碳酸钙处理土壤DOC含量在15

35 ℃下分别显著提高83.41%

80.37%

90.41%;添加碳酸钙处理温度对土壤DOC、MBC具有显著影响(p＜0.05)

在培养的第15

60天

土壤DOC和MBC含量均在不同温度下达到显著性差异

土壤DOC含量大小依次为35

25 ℃

土壤MBC含量多少依次为15

35 ℃。同位素标记发现

在培养第1和第5天

13C-DOC

13C-MBC含量在15

35 ℃下达到峰值

且13C-DOC对土壤总DOC贡献率分别为16.85%

21.20%和15.22%

13C-MBC对土壤总MBC的贡献率分别11.95%

10.49%和17.18%。添加碳酸钙各处理均显著提高土壤pH。高通量测序发现

在培养第60天

外源碳酸钙输入对细菌群落组成相对丰度及群落结构具有显著影响

但对真菌群落组成影响较小。添加碳酸钙处理提高变形菌门相对丰度

降低绿弯菌门相对丰度。相关分析表明

土壤pH与优势菌门变形菌门(Proteobacteria)、担子菌门(Basidiomycota)呈显著正相关

与绿弯菌门(Chloroflexi)、子囊菌门(Ascomycota)呈显著负相关;绿弯菌门与土壤DOC呈显著负相关

放线菌门与土壤MBC、13C-DOC及担子菌门与DOC、13C-MBC、13C-DOC呈显著正相关。综上表明

外源碳酸钙对土壤活性有机碳的影响大于温度

外源碳酸钙增加土壤pH、DOC和MBC含量

改变土壤微生物群落组成丰度。因此

碳酸钙添加是黄壤有机碳固存和改良的有效措施。

Abstract

In order to explore the effects of adding calcium carbonate on active organic carbon components and microbial community composition under different temperature conditions

the typical yellow soil in Guizhou province was taken as the research object

and six treatments were set up (the treatments with and without adding calcium carbonate at culture temperatures of 15

35 ℃) by isotope labeling method. By analyzing the effects of calcium carbonate addition on soil active organic carbon and microbial community composition at different temperatures

the distribution rule of foreign carbon in soil active carbon pool was revealed

with a view to providing a theoretical basis for organic carbon sequestration and improvement of typical yellow soil in Guizhou. The results showed that: The addition of calcium carbonate significantly increased the contents of DOC and MBC in soil at different temperatures (p<0.01)

and the contents of DOC in soil treated with calcium carbonate reached the maximum value on the 5th day of culture. Compared with the treatment without calcium carbonate

the soil DOC content in the treatment with calcium carbonate were significantly increased by 83.41%

80.37% and 90.41% at 15

25 and 35 ℃

respectively. The temperature of calcium carbonate addition had significant effects on soil DOC and MBC (p<0.05). On the 15th and 60th day of culture

the contents of DOC and MBC in soil showed significant differences at different temperatures

and the overall content of DOC in soil was 35 ℃＞15 ℃＞25 ℃

and the overall content of MBC was 15 ℃＞25 ℃＞35 ℃. Isotopic labeling showed that the contents of 13C-DOC and 13C-MBC peaked at 15

25 and 35 ℃ on the 1st and 5th day of culture

and the contribution rate of 13C-DOC to the total soil DOC was 16.85%

21.20% and 15.22%

respectively. The contribution rate of 13C-MBC to total soil MBC was 11.95%

10.49% and 17.18%

respectively. The addition of calcium carbonate significantly increased soil pH. High-throughput sequencing showed that the input of exogenous calcium carbonate had significant effects on the relative abundance and community structure of bacterial community composition

but had little effect on fungal community composition at day 60 of culture. The addition of calcium carbonate increased the relative abundance of Proteobacteria and decreased the relative abundance of Chlorocurvula. Correlation analysis showed that soil pH was positively correlated with the dominant bacteria Proteobacteria and Basidiomycota

and negatively correlated with Chloroflexi and Ascomycota. The chloromycetes was significantly negatively correlated with soil DOC

while the actinomycetes was significantly positively correlated with soil MBC

13C-DOC and basidiomycetes was significantly positively correlated with DOC

13C-MBC and 13C-DOC. In conclusion

the effect of exogenous calcium carbonate on soil active organic carbon was greater than that of temperature. Exogenous calcium carbonate increased soil pH

soil DOC and MBC contents

and changed the composition and abundance of soil microbial community. Therefore

calcium carbonate addition is an effective measure for sequestration and improvement of organic carbon in yellow soil.

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references

苗其硕.贵州黄壤的利用改良和综合治理[J].贵州农业科学,1982,10(5):11-15,5.

Chan K Y, Conyers M K, Scott B J. Improved structural stability of an acidic hardsetting soil attributable to lime application[J].Communications in Soil Science and Plant Analysis,2007,38(15/16):2163-2175.

林仕芳,王小利,段建军,等.有机肥替代化肥对旱地黄壤有机碳矿化及活性有机碳的影响[J].环境科学,2022,43(4):2219-2225.

Benbi D K, Brar K, Toor A S, et al. Sensitivity of labile soil organic carbon pools to long-term fertilizer, straw and manure management in rice-wheat system[J].Pedosphere,2015,25(4):534-545.

Dikgwatlhe S B, Chen Z D, Lal R, et al. Changes in soil organic carbon and nitrogen as affected by tillage and residue management under wheat-maize cropping system in the North China Plain[J].Soil and Tillage Research,2014,144:110-118.

Guo A N, Ding L J, Tang Z, et al. Microbial response to CaCO

application in an acid soil in Southern China[J].Journal of Environmental Sciences,2019,79:321-329.

李梦丽,徐墨馨,陈永山,等.石灰性土壤添加不同量碳酸钙对秸秆有机碳矿化的影响[J].生态环境学报,2022,31(10):2002-2009.

Feng S Z, Huang Y, Ge Y H, et al. Variations in the patterns of soil organic carbon mineralization and microbial communities in response to exogenous application of rice straw and calcium carbonate[J].Science of the Total Environment,2016,571:615-623.

王莲阁,高岩红,丁长欢,等.变温环境对典型石灰土有机碳矿化的影响[J].环境科学,2014,35(11):4291-4297.

漆良华,范少辉,杜满义,等.湘中丘陵区毛竹纯林、毛竹-杉木混交林土壤有机碳垂直分布与季节动态[J].林业科学,2013,49(3):17-24.

曹建华,潘根兴,袁道先,等.岩溶地区土壤溶解有机碳的季节动态及环境效应[J].生态环境,2005,14(2):224-229.

Huang Q, Wang J L, Wang C, et al. The 19-years inorganic fertilization increased bacterial diversity and altered bacterial community composition and potential functions in a paddy soil[J].Applied Soil Ecology,2019,144:60-67.

张义杰,徐杰,陆仁窗,等.生石灰对林下酸化土壤的调控作用及三七生长的影响[J].应用生态学报,2022,33(4):972-980.

李新华,郭洪海,朱振林,等.不同秸秆还田模式对土壤有机碳及其活性组分的影响[J].农业工程学报,2016,32(9):130-135.

鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2000.

周兴,廖育林,鲁艳红,等.肥料减施条件下水稻土壤有机碳组分对紫云英-稻草协同利用的响应[J].水土保持学报,2017,31(3):283-290.

郭万里,武均,蔡立群,等.不同氮素水平下生物质炭、秸秆添加对陇中黄土高原旱作农田土壤活性有机碳的影响[J].水土保持学报,2020,34(1):283-291.

葛云辉,邹冬生,苏以荣,等.外源碳酸钙和稻草对喀斯特地区土壤活性有机碳的影响[J].湖南农业大学学报(自然科学版),2012,38(3):314-318.

董远鹏,刘喜娟,董梦阳,等.腐殖质和硝酸钙对赤泥团聚体形成的促进作用[J].环境污染与防治,2020,42(10):1205-1210.

徐学池,黄媛,何寻阳,等.土壤水分和温度对西南喀斯特棕色石灰土无机碳释放的影响[J].环境科学,2019,40(4):1965-1972.

Fernández-Calviño D, Bååth E. Growth response of the bacterial community to pH in soils differing in pH[J].FEMS Microbiology Ecology,2010,73(1):149-156.

刘帅,陈玥希,孙辉,等.西南亚高山-高山海拔梯度上森林土壤水溶性有机碳时间动态[J].西北林学院学报,2015,30(1):33-38.

张勇,胡海波,王增,等.凤阳山4种森林土壤在不同温度培养下活性有机碳的变化[J].浙江农林大学学报,2018,35(2):243-251.

Xun W B, Huang T, Zhao J, et al. Environmental conditions rather than microbial inoculum composition determine the bacterial composition, microbial biomass and enzymatic activity of reconstructed soil microbial communities[J].Soil Biology and Biochemistry,2015,90:10-18.

王智慧,蒋先军.紫色土中微生物群落结构及功能特征对土壤pH的差异响应[J].环境科学,2022,43(7):3876-3883.

Yan S S, Song J M, Fan J S, et al. Changes in soil organic carbon fractions and microbial community under rice straw return in Northeast China[J].Global Ecology and Conservation,2020,22:e00962.

李慧敏,田胜营,李丹丹,等.有机物料施用对潮土活性有机碳及微生物群落组成的影响[J].土壤学报,2021,58(3):777-787.

Zheng Q, Hu Y T, Zhang S S, et al. Soil multifunctionality is affected by the soil environment and by microbial community composition and diversity[J].Soil Biology and Biochemistry,2019,136:e107521.

Tian K, Zhao Y C, Xu X H, et al. Effects of long-term fertilization and residue management on soil organic carbon changes in paddy soils of China: A meta-analysis[J].Agriculture, Ecosystems and Environment,2015,204:40-50.

Fierer N, Bradford M A, Jackson R B. Toward an ecological classification of soil bacteria[J].Ecology,2007,88(6):1354-1364.

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