福建典型茶园土壤养分及其计量比对细菌群落结构的影响

杨发峻; 王维奇; 吴梓炜; 许宏达; 葛茂泉; 林少颖; 李先德; 张永勋; 吴传惠

doi:10.13870/j.cnki.stbcxb.2023.06.027

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福建典型茶园土壤养分及其计量比对细菌群落结构的影响

更新时间：2025-03-12

- 福建典型茶园土壤养分及其计量比对细菌群落结构的影响
- Effects of Soil Nutrient and MeasurementRatio on Bacterial Community Structure in Fujian Tea Garden
- 水土保持学报 2023年37卷第6期页码：209-218
- 作者机构：
  
  1. 福建师范大学湿润亚热带生态-地理过程教育部重点实验室,福州,350117
  2. 福建师范大学地理研究所,福州,350117
  3. 中国农业科学院农业经济与发展研究所,北京,100081
  4. 福建顺茗道茶业有限公司,福建,宁德,355200
- 作者简介：
  
  [ "杨发峻(1999-),男,在读硕士研究生,主要从事生态与环境研究。E-mail:yfj150315@163.com" ]
- 基金信息：
  
  国家自然科学基金面上项目(41571287);福建福鼎白茶文化系统全球重要农业文化遗产申报项目(DH-1815);农业农村部全球重要农业文化遗产申报与国际交流项目(12190036)
- DOI：10.13870/j.cnki.stbcxb.2023.06.027
  中图分类号：
- 纸质出版：2023
- 稿件说明：
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杨发峻, 王维奇, 吴梓炜, 等. 福建典型茶园土壤养分及其计量比对细菌群落结构的影响[J]. 水土保持学报, 2023,37(6):209-218.

YANG Fajun, WANG Weiqi, WU Ziwei, et al. Effects of Soil Nutrient and MeasurementRatio on Bacterial Community Structure in Fujian Tea Garden[J]. 2023, 37(6): 209-218.
杨发峻, 王维奇, 吴梓炜, 等. 福建典型茶园土壤养分及其计量比对细菌群落结构的影响[J]. 水土保持学报, 2023,37(6):209-218. DOI： 10.13870/j.cnki.stbcxb.2023.06.027.

YANG Fajun, WANG Weiqi, WU Ziwei, et al. Effects of Soil Nutrient and MeasurementRatio on Bacterial Community Structure in Fujian Tea Garden[J]. 2023, 37(6): 209-218. DOI： 10.13870/j.cnki.stbcxb.2023.06.027.

摘要

为研究茶园土壤碳(C)、氮(N)、磷(P)、钾(K)含量及其生态化学计量比对土壤微生物群落结构的影响

以福建省福鼎市茶叶主产地茶园为研究对象

系统测定和分析土壤养分与计量比

分析其对细菌群落特征的影响。结果表明:(1)茶园土壤C、N、P、K含量均值分别为18.39

1.96

0.91

15.57 g/kg

土壤C∶N、C∶P、C∶K、N∶P、N∶K、P∶K均值分别为9.24

30.96

1.24

3.32

0.13

22.39;(2)土壤中的优势菌门为变形菌门(Proteobacteria)、酸杆菌门(Actinobacteria)、放线菌门(Acidobacteria);优势菌属为Paludibaculum、分枝杆菌属(Mycobacterium)、慢生根瘤菌属(Bradyrhizobium)

不同茶园土壤中细菌群落组成及结构相似

但细菌多样性指数差异显著(p＜0.05);(3)属水平上茶园土壤细菌与养分及其计量比的相关性分析结果显示

K与热脱硫弧菌属(Thermodesulfovibrio)、盖氏菌属(Gaiella)呈显著正相关(p＜0.01)

P与梭菌属(Clostridium)、罗思河小杆菌属(Rhodanobacter)、地杆菌属(Geobacter)呈显著正相关(p＜0.01)

而N与假单胞菌属(Pseudomonas)呈显著负相关(p＜0.01)

K与北孢里菌属(Kitasatospora)、苯基杆菌属(Phenylobacterium)呈显著负相关(p＜0.01);土壤C∶N、C∶P、N∶P、P∶K与西索恩氏菌属(Chthoniobacter)、网团菌属(Dictyobacter)、伯克霍尔德菌属(Paraburkholderia)、芽孢杆菌属(Bacillus)呈显著正相关(p<0.05)

与慢生根瘤菌属(Bradyrhizobium)、Vicinamibacter、盖氏菌属(Gaiella)、假单胞菌属(Pseudomonas)等呈显著负相关(p<0.001);(4)功能预测结果显示

茶园细菌的预测功能中以代谢活动为主的功能居多

对茶园土壤养分循环以及通过代谢活动提高土壤固碳效率有着积极作用。研究结果对基于养分管理的茶园土壤微生物多样性功能研究具有指导意义。

Abstract

In order to study the effects of soil carbon(C)

nitrogen (N)

phosphorus (P)

potassium (K) content and their eco stoichiometric ratio on soil microbial community structure

soil nutrient-to-metrological ratio and its influence on the characteristics of bacterial communities were systematically measured and analyzed by taking tea gardens in Fuding City

Fujian Province as the research object. The results showed that: (1) The mean contents of C

P and K in tea garden soil were 18.39

1.96

0.91

15.57 g/kg

and the mean values of soil C∶N

C∶P

C∶K

N∶P

N∶K

were 9.24

1.24

3.32

0.13 and 22.39

respectively. (2) The dominant bacteria in the soil were Paludibaculum

Mycobacterium

and Acidobacteria; the dominant bacteria genera were Paludibaculum

Mycobacterium and Bradyrhizobium

and the composition and structure of bacterial communities in different tea plantations were similar

but the bacterial diversity index was significantly different (p<0.05). (3) The correlation analysis results of soil bacteria and nutrient and their measurement ratio in tea gardens at the genus level showed that K was significantly positively correlated with Thermodesulfovibrio and Gaiella (p<0.01)

P was positively correlated with Clostridium

Rhodanobacter

and Geobacter (p<0.001)

while N was significantly negatively correlated with Pseudomonas (p<0.01)

K was significantly negatively correlated with Kitasatospora and Phenylobacterium (p<0.01). Soil C∶N

C∶P

N∶P

P∶K were significantly positively correlated with Chthoniobacter

Dictyobacter

Paraburkholderia

and Bacillus (p<0.05)

and were negatively correlated with Bradyrhizobium

Vicinamibacter

Gaiella

and Pseudomonas (p<0.001). (4) The functional prediction results showed that most of the predicted functions of tea garden bacteria were mainly metabolic activities

which had a positive effect on soil nutrient cycling in tea gardens and improving soil carbon sequestration efficiency through metabolic activities. Therefore

this study is of great significance for the study of soil microbial diversity in tea gardens based on nutrient management.

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references

陈晓旋,陈淑云,曾从盛,等.螃蟹对闽江河口湿地土壤碳氮磷含量及其生态化学计量学特征影响[J].环境科学学报,2018,38(3):1179-1188.

王维奇,王纯,曾从盛,等.闽江河口不同河段芦苇湿地土壤碳氮磷生态化学计量学特征[J].生态学报,2012, 32(13):4087-4093.

许窕孜,叶彩红,张耕,等.北江中下游不同林分类型土壤C、N、P生态化学计量特征[J].应用生态学报,2023,34(4):962-968.

王涛,万晓华,程蕾,等.杉木采伐迹地营造阔叶树种对土壤微生物生态化学计量特征的影响[J].应用生态学报,2020,31(11):3851-3858.

王珍,金轲,丁勇,等.植物-土壤微生物反馈在草地演替过程中的作用机制[J].中国草地学报,2022,44(1):95-103.

许越,杨杰,陈闻,等.南古红壤坡地人工林生态系统土壤细菌群落结构与丰度季节变化特征[J].南方农业学报,2018.49(7):1289-1296.

何柳,曹敏敏,鲁建兵,等.浙江凤阳山不同海拔常绿阔叶林土壤微生物特征[J].浙江农林大学学报,2022,39(6):1267-1277.

刘银秀,池永清,董越勇,等.不同沼液施用年限土壤养分含量和微生物群落结构差异[J].植物营养与肥料学报,2023,29(3):483-495.

郑宗贤,刘洋,唐志勇.福鼎市雷暴气候特征及气象防雷减灾对策[J].农业灾害研究,2022,12(7):78-80.

鲁如坤.土壤农业化学分析方法[M].北京:中国农业科技出版社,2000.

李朝英,郑路.流动分析仪同时快速测定植物全氮、全磷含量的方法改进[J].中国土壤与肥料,2021(2):336-342.

杜雄雁,杨明芳,余浪,等.微波碱熔消解快速测定土壤中全钾[J].云南化工,2022,49(8):85-87.

Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities[J].Applied and Environmental Microbiology,2009,75(23):7537-7541.

Wang Q, Garrity G M, Tiedje J M, et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.

中华人民共和国农业行业标准,NY/T 853-2004,茶叶产地环境技术条件[S].北京:中华人民共和国农业部,2004.

Tian H Q, Chen G S, Zhang C, et al. Pattern and variation of C∶N∶P ratios in China’s soils: A synthesis of observational data[J].Biogeochemistry,2010,98(1):139-151.

Yergeau E, Bokhorst S, Kang S, et al. Shifts in soil microorganisms in response to warming are consistent across a range of Antarctic environments[J].The ISME Journal,2012,6(3):692-702.

Ventura M, Canchaya C, Tauch A, et al. Genomics of

Actinobacteria

: Tracing the evolutionary history of an ancient Phylum[J].Microbiology and Molecular Biology Reviews,2007,71(3):495-548.

苏梦迪,马啸,胡丽涛,等.高碳基肥减氮施用对土壤肥力和细菌多样性的影响[J].农业生物技术学报,2022,30(6):1174-1185.

Rilling J I, Acuña J J, Sadowsky M J, et al. Putative nitrogen-fixing bacteria associated with the rhizosphere and root endosphere of wheat plants grown in an Andisol from southern Chile[J].Frontiers in Microbiology,2018,9:e2710.

杨顺,杨婷,林斌,等.两株溶磷真菌的筛选、鉴定及溶磷效果的评价[J].微生物学报,2018,58(2):264-273.

Song M, Yun H Y, Kim Y H. Antagonistic

Bacillus

species as a biological control of ginseng root rot caused by

Fusarium

incarnatum

.Journal of Ginseng Research,2014,38(2):136-145.

康慎敏,武瑞赟,穆文强,等.优良植物根际促生菌的筛选及其生物学特性[J].中国农业大学学报,2023,28(1):137-152.

马胜兰,况福虹,林洪羽,等.秸秆还田量对川中丘陵冬小麦-夏玉米轮作体系土壤物理特性的影响[J].中国农业科学,2023,56(7):1344-1358.

吴志丹,江福英,尤志明,等.福建省安溪县铁观音茶园土壤钾素状况[J].茶叶学报,2018,59(1):26-32.

吴桐桐,徐基胜,周云鹏,等.黄河三角洲不同生境土壤理化特性及细菌群落结构特征[J].农业环境科学学报, 2022,41(10):2250-2261.

Yao M J, Rui J P, Li J B, et al. Rate-specific responses of prokaryotic diversity and structure to nitrogen deposition in the

Leymus chinensis

steppe[J].Soil Biology and Biochemistry,2014,79:81-90.

韦双,韩小美,黄伟,等.望天树人工林根际溶磷细菌的筛选及溶磷特性[J].北京林业大学学报,2023,45(3):79-92.

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