功能菌剂与生物炭配施对沙化土壤的影响

王红萍; 步连燕; 陈文峰; 安德荣; 张应龙; 韦革宏; 王红雷

doi:10.13870/j.cnki.stbcxb.2023.01.044

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功能菌剂与生物炭配施对沙化土壤的影响

更新时间：2025-03-12

- 功能菌剂与生物炭配施对沙化土壤的影响
- Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil
- 水土保持学报 2023年37卷第1期页码：345-353
- 作者机构：
  
  1. 西北农林科技大学水土保持研究所, 陕西杨凌,712100
  2. 西北农林科技大学生命科学学院, 旱区作物逆境生物学国家重点实验室, 陕西农业与环境微生物重点实验室, 陕西杨凌,712100
  3. 中国农业大学生物学院,北京,100193
  4. 西北农林科技大学植物保护学院, 陕西杨凌,712100
  5. 陕西省神木市生态保护建设协会, 陕西神木,719399
  6. 中国科学院水利部水土保持研究所, 陕西杨凌,712100
- 作者简介：
  
  [ "王红萍(1998-),女,硕士研究生,主要从事生态修复研究。E-mail:2020051501@nwafu.edu.cn" ]
- 基金信息：
  
  陕西省重点研发计划重点产业创新链项目(2020ZDLNY07-07);中国科学院"西部之光"人才培养引进计划项目(XAB2019A05)
- DOI：10.13870/j.cnki.stbcxb.2023.01.044
  中图分类号： S154.3
- 纸质出版：2023
- 稿件说明：
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王红萍, 步连燕, 陈文峰, 等. 功能菌剂与生物炭配施对沙化土壤的影响[J]. 水土保持学报, 2023,37(1):345-353.

WANG Hongping, BU Lianyan, CHEN Wenfeng, et al. Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil[J]. 2023, 37(1): 345-353.
王红萍, 步连燕, 陈文峰, 等. 功能菌剂与生物炭配施对沙化土壤的影响[J]. 水土保持学报, 2023,37(1):345-353. DOI： 10.13870/j.cnki.stbcxb.2023.01.044.

WANG Hongping, BU Lianyan, CHEN Wenfeng, et al. Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil[J]. 2023, 37(1): 345-353. DOI： 10.13870/j.cnki.stbcxb.2023.01.044.

摘要

研究生物炭和功能菌剂协同调控沙化土壤养分和微生物多样性与功能

可为沙化土壤肥力培育提供理论依据。选择毛乌素沙地典型生态经济林沙化土壤为研究对象

采用田间定位试验

运用高通量扩增测序技术

解析不同添加量(2%

8%)生物炭处理及其配施定量功能菌剂(枯草芽孢杆菌+巨大芽孢杆菌+胶质芽孢杆菌)处理对土壤化学性质及细菌菌群多样性与功能的影响。结果表明:(1)与单一生物炭处理相比

生物炭+功能菌剂处理组土壤有效氮、磷养分含量分别提升44.71%和187.36%;(2)生物炭单施或者配施功能菌剂均使酸杆菌门相对丰度显著降低

而生物炭+功能菌剂处理中厚壁菌门的相对丰度

分别增加163.80%

155.15%

100.21%

并且不同程度上调土壤细菌物种丰富度和多样性;(3)功能菌剂与生物炭配施改变土壤细菌介导的碳氮循环功能

如显著提高土壤细菌的有机物质分解功能

壳聚糖和木聚糖分解过程较对照组分别上升186.54%

242.46%

增强细菌的氨化和呼吸功能

而单施生物炭处理提升细菌的反硝化与固氮功能。综上

生物炭和功能菌剂的添加提高土壤有效氮磷养分含量

显著改变土壤细菌群落多样性与群落功能

生物炭配施功能菌剂优于单施生物炭处理

该结果可为沙化土壤改良方法的选择提供科学依据和理论参考。

Abstract

Investigating the biochar and functional fungal agents to coordinate the regulation of desertified soil nutrients and microbial diversity and function can provide a theoretical basis for the fertility cultivation of desertified soil. The soil of the typical ecological and economic forest of the Mu Wusu Sandy Land was selected as the research object

and the effects of different amounts (2%

8%) of biochar treatment and its combined application of quantitative functional agent treatment (Bacillus subtilis + Bacillus megaterium + Bacillus colloid) on soil chemical properties

bacterial diversity and function were analyzed by field positioning experiment and high-throughput amplification and sequencing technology. The results showed that: (1) Compared with the single biochar treatment

the soil available nitrogen and phosphorus nutrient contents of the biochar + functional agent treatment group increased by 44.71% and 187.36%

respectively. (2) The relative abundance of the Acidobacteria was significantly reduced by the single application or combination of functional agents of biochar

while the relative abundance of the phylum Firmcutes in the treatment of biochar + functional bacteria increased by 163.80%

155.15%

and 100.21%

respectively

and the richness and diversity of soil bacterial species were increased to varying degrees. (3) The combined application of functional agents and biochar changed the carbon and nitrogen cycle function mediated by soil bacteria

such as significantly improving the decomposition function of organic matter of soil bacteria

and the decomposition process of chitosan and xylan increased by 186.54% and 242.46% respectively compared with the control group; enhanced the ammonization and respiratory function of bacteria; while single biochar treatment improved the denitrification and nitrogen fixation function of the bacteria. In summary

biochar with functional bacterial agents is better than single application of biochar treatment. Biochar and functional bacteria agents added to improve the soil available nitrogen and phosphorus nutrient content

significantly changed the soil bacterial community diversity and community function. These results can provide a scientific basis and theoretical reference for the selection of desertification soil improvement methods.

关键词

Keywords

references

朱灵,李易,杨婉秋,等.沙化对高寒草地土壤碳、氮、酶活性及细菌多样性的影响[J].水土保持学报,2021,35(3):350-358.

Milkereit J, Geisseler D, Lazicki P, et al. Interactions between nitrogen availability,bacterial communities,and nematode indicators of soil food web function in response to organic amendments[J].Applied Soil Ecology, 2021,157:e103767.

江仁涛,李富程,沈凇涛,等.不同年限红柳恢复川西北高寒沙地对土壤团聚体和有机碳的影响[J].水土保持学报,2018,32(1):197-203.

刘文辉,梁翔宇,孙小涵,等.生物质炭作为土壤改良剂在农业上的应用研究进展[J].中国资源综合利用,2020,38(6):108-110.

闻艺,王昌梅,张无敌,等.生物炭的研究现状与农业推广应用分析[J].安徽农业科学,2021,49(22):10-13,17.

Kuzyakov Y, Bogomolova I, Glaser B. Biochar stability in soil:Decomposition during eight years and transformation as assessed by compound-specific 14C analysis[J].Soil Biology and Biochemistry,2014,70:229-236.

Novak J M, Lima I, Xing B, et al. Characterization of designer biochar produced at different temperatures and their effects on a loamy sand[J].Annals of Environmental Science,2009,3(11):195-206.

王湛,李银坤,徐志刚,等.生物质炭对土壤理化性状及氮素转化影响的研究进展[J].土壤,2019,51(5):835-842.

许云翔,何莉莉,陈金媛,等.生物炭对农田土壤氨挥发的影响机制研究进展[J].应用生态学报,2020,31(12):4312-4320.

罗锋,杭中桥,焦玉霞,等.农用微生物菌剂在蔬菜上的应用试验[J].中国园艺文摘,2009,25(8):39-40.

李俊,姜昕,李力等.微生物肥料的发展与土壤生物肥力的维持[J].中国土壤与肥料,2006(4):1-5.

杨涛,姚阳阳,王治业,等.复合菌剂调控连作当归根围土壤养分及对产量的影响[J].微生物学通报,2022,49(7):2648-2660.

Wang J J, Li Q Q, Xu S, et al. Traits-based integration of multi-species inoculants facilitates shifts of indigenous soil bacterial community//.中国土壤学会土壤环境专业委员会,第二十次会议暨农田土壤污染与修复研讨会摘要集.合肥,2018:141-142.

王岩,周鹏,白立伟,等.生物炭和AM真菌配施对连作辣椒生长和土壤养分的影响[J].中国生态农业学报(中英文),2020,28(10):1600-1608.

李卓瑞,韦高玲.不同生物炭添加量对土壤中氮磷淋溶损失的影响[J].生态环境学报,2016,25(2):333-338.

陈心想,何绪生,耿增超,等.生物炭对不同土壤化学性质、小麦和糜子产量的影响[J].生态学报,2013,33(20):6534-6542.

王明湖,连瑛,李雅颖,等.不同微生物菌剂对草莓炭疽病防控和土壤微生物群落结构的影响[J].浙江农业科学,2022,63(4):783-786.

赵晓军,李丽,张璇,等.生物炭与微生物菌剂配施对土壤生物和化学特性的影响[J].安徽农业科学,2018,46(25):109-112.

鲍士旦.土壤农化分析[M].3版.北京:中国农业出版社,2010.

环境保护部.中华人民共和国国家环境保护标准《HJ802-2016-土壤电导率的测定电极法》[S].北京:中国环境科学出版社,2016.

Victoria N, Tobias R, Dries H, et al. Maize biochars accelerate short-term soil nitrogen dynamics in a loamy sand soil[J].Soil Biol and Biochemistry,2012,55:20-27.

唐行灿,陈金林.生物炭对土壤理化和微生物性质影响研究进展[J].生态科学,2018,37(1):192-199.

Robertson S J, Rutherford P M, López-Gutiérrez J C, et al. Biochar enhances seedling growth and alters root symbioses and properties of sub-boreal forest soils[J].Canadian Journal of Soil Science,2012,92(2):329-340.

张清敏,刘曼,周湘婷.微生物肥料在土壤生态修复中的作用[J].农业环境科学学报,2006,25(增刊1):283-284.

毛晓曦,管培彬,马理,等.有机物料配施菌剂对设施次生盐渍化土壤的修复效果[J].中国农学通报,2021,37(26):80-87.

Yang X, Post W M, Thornton P E, et al.The distribution of soil phosphorus for global biogeochemical modeling[J].Biogeosciences,2013,10(4):2525-2537.

Hatzinger P B, Alexander M. Relationship between the number of bacteria added to soil or seeds and their abundance and distribution in the rhizosphere of alfalfa[J].Plant and Soil,1994,158(2):211-222.

程扬,刘子丹,沈启斌,等.秸秆生物炭施用对玉米根际和非根际土壤微生物群落结构的影响[J].生态环境学报,2018,27(10):1870-1877.

陈昕,李奇,陈懂懂,等.三江源国家公园不同草地土壤微生物功能基因的差异性分析[J].生态环境学报,2020,29(3):472-482.

吴希慧,王蕊,高长青,等.土地利用驱动的土壤性状变化影响微生物群落结构和功能[J].生态学报.2021,41(20):7989-8002.

褚义红,崔世茂,付崇毅,等.不同微生物菌肥对生菜生长及品质的影响[J].蔬菜,2014(3):20-24.

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