色季拉山不同海拔土壤微生物及真菌群落组成特征

曹丽花; 刘合满; 杨红; 连玉珍

doi:10.13870/j.cnki.stbcxb.2022.06.045

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色季拉山不同海拔土壤微生物及真菌群落组成特征

更新时间：2025-03-12

- 色季拉山不同海拔土壤微生物及真菌群落组成特征
- Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet
- 水土保持学报 2022年36卷第6期页码：371-378
- 作者机构：
  
  1. 信阳农林学院林学院,河南,信阳,464000
  2. 西藏农牧学院资源与环境学院,西藏,林芝,860000
  3. 周口市农业农村局土壤肥料工作站,河南,周口,466099
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(41561052,41461055);河南省科技攻关项目(202102310030,212102310981)
- DOI：10.13870/j.cnki.stbcxb.2022.06.045
  中图分类号： S154.37
- 纸质出版：2022
- 稿件说明：
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曹丽花, 刘合满, 杨红, 等. 色季拉山不同海拔土壤微生物及真菌群落组成特征[J]. 水土保持学报, 2022,36(6):371-378.

CAO Lihua, LIU Heman, YANG Hong, et al. Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet[J]. 2022, 36(6): 371-378.
曹丽花, 刘合满, 杨红, 等. 色季拉山不同海拔土壤微生物及真菌群落组成特征[J]. 水土保持学报, 2022,36(6):371-378. DOI： 10.13870/j.cnki.stbcxb.2022.06.045.

CAO Lihua, LIU Heman, YANG Hong, et al. Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet[J]. 2022, 36(6): 371-378. DOI： 10.13870/j.cnki.stbcxb.2022.06.045.

摘要

为探究不同海拔高度土壤微生物的分布特征

以西藏东南部色季拉山为研究区

以不同海拔高度(4 606

4 110

4 000

3 900

3 440 m)土壤为研究对象

分析0—5

5—10 cm 2个层次土壤可培养微生物数量分布特征

并采用高通量测序技术分析了真菌群落组成的丰富度和多样性

探讨了真菌群落组成的主要影响因素。结果表明

供试区土壤可培养微生物数量表现为细菌＞放线菌＞真菌

真菌数量与海拔高度显著相关

而细菌和放线菌数量随海拔高度无明显变化规律;5个海拔高度下共检测到真菌4个门、18个纲、47个目、74个科、93个属;在门水平上优势真菌为担子菌门(Basidiomycota)和子囊菌门(Ascomycota)

纲分类水平上担子菌门下的伞菌纲(Agaricomycetes)相对丰度最高。Alpha多样性分析表明

低海拔区川滇高山栎林和高海拔区高寒草地土壤真菌丰度和多样性均较高

海拔3 900~4 110 m的中部海拔区的雪山杜鹃和急尖长苞冷杉林土壤则较低;冗余分析发现

在门和纲分类水平上

土壤有机碳和全氮含量是真菌群落差异的主要影响因素

海拔高度的影响相对较小。研究结果表明

微生物在不同海拔高度上的分布可能主要取决于土壤性质和植被类型。

Abstract

In order to elucidate the distribution characteristics of soil microorganisms at different altitudes

we selected Sejila mountain as a model area

a typical forest ecological area in southeastern Tibet. In this study we investigated the cultivable microorganisms and the richness of fungal community composition and relative abundances along an altitude gradient (approximately 3 400~4 600 m) in Sejila mountain. The amount of soil cultivable microorganisms in the 0—5 and 5—10 cm layer showed the following rank order: bacteria＞actinomycetes＞fungi

and the number of fungi was significantly correlated with altitude

while the number of bacteria and actinomycetes had no obvious change with altitude. The high-throughput sequencing results showed that there were 4 phyla

18 classes

47 orders

74 families

and 93 genera of fungi detected. Basidiomycota and Ascomycota were predominant fungal at the phylum level

and Agaricomycetes had the highest relative abundance at the classes level. Alpha diversity analysis showed that the soil fungal abundance and diversity of the Quercus aquifolioides forest in lower altitude and the alpine grassland in the higher altitude area were higher

while the Rhododendron aganiphum and Abies georgei var. smithii forest in the middle altitude (3 900~4 110 m) was lower. The redundancy analysis found that

at the phylum and class classification level

the fungal community was mainly caused by soil organic carbon and total nitrogen contents

while the influence of altitude was relatively small. The results suggest that the distribution of microorganisms at different elevations may mainly depend on soil properties and vegetation type.

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