微生物诱导碳酸钙沉淀对干旱半干旱区铜尾矿污染治理效果

常道琴; 宋乃平; 岳健敏; 孟晨; 马昀

doi:10.13870/j.cnki.stbcxb.2022.04.045

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微生物诱导碳酸钙沉淀对干旱半干旱区铜尾矿污染治理效果

更新时间：2025-03-12

- 微生物诱导碳酸钙沉淀对干旱半干旱区铜尾矿污染治理效果
- Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area
- 水土保持学报 2022年36卷第4期页码：365-374
- 作者机构：
  
  1. 宁夏大学生态环境学院,银川,750021
  2. 宁夏大学西北土地退化与生态系统恢复国家重点实验室培训基地,银川,750021
  3. 西北退化生态系统恢复与重建教育部重点实验室,银川,750021
- 作者简介：
  
  [ "常道琴(1995—),女,在读硕士研究生,主要从事恢复生态学研究。E-mail:2213582962@qq.com" ]
- 基金信息：
  
  国家重点研发项目（2018YFC1802902）宁夏自然科学基金优青项目（2022AAC05005）宁夏自然科学基金项目（2020AAC03091）
- DOI：10.13870/j.cnki.stbcxb.2022.04.045
  中图分类号： X53
- 纸质出版：2022
- 稿件说明：
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常道琴, 宋乃平, 岳健敏, 等. 微生物诱导碳酸钙沉淀对干旱半干旱区铜尾矿污染治理效果[J]. 水土保持学报, 2022,36(4):365-374.

CHANG Daoqin, SONG Naiping, YUE Jianmin, et al. Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area[J]. 2022, 36(4): 365-374.
常道琴, 宋乃平, 岳健敏, 等. 微生物诱导碳酸钙沉淀对干旱半干旱区铜尾矿污染治理效果[J]. 水土保持学报, 2022,36(4):365-374. DOI： 10.13870/j.cnki.stbcxb.2022.04.045.

CHANG Daoqin, SONG Naiping, YUE Jianmin, et al. Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area[J]. 2022, 36(4): 365-374. DOI： 10.13870/j.cnki.stbcxb.2022.04.045.

摘要

为明确微生物诱导碳酸钙沉淀技术（microbial induced calcium carbonate precipitation，MICP）在干旱半干旱高风蚀地区重金属尾矿治理效果，以研究区生境土中筛选的碳酸盐矿化细菌—纺锤型赖氨酸芽孢杆菌（Lysinibacillus fusiformis）为研究对象，通过在尾矿渣中加注纺锤型赖氨酸芽孢杆菌菌液和由1 mol/L的尿素及氯化钙配制成的胶结液进行室内模拟试验，研究MICP处理前后尾矿坡顶、坡中、坡底（坡顶—细颗粒（K1<5 mm）、坡中—中颗粒（5 mm15 mm））不同粒径尾矿渣中重金属元素含量、pH、电导率及酶活性等基础理化性质的变化，利用双因素方差分析和相关性热图比较分析揭示基于生境微生物的MICP修复技术对区域不同粒径尾矿渣污染物治理的效果及确定MICP技术对不同粒径尾矿渣修复的菌剂用量。结果表明，采用MICP技术对尾矿渣中重金属含量、pH、电导率及酶活性等基础理化性质有显著影响。MICP处理下细颗粒（K1<5 mm）尾矿渣中As降低21%，中颗粒（5 mm15 mm）中Pb降低22%。K1、K3中的pH降低5%，K2降低4%。K1中全钾、脲酶均升高33%（p<0.05），K2中全碳升高39%（p<0.05），K3中蔗糖酶升高7%（p<0.05），微生物碳氮在K1、K2、K3中显著升高（p<0.05）。同时MICP处理下Ni与Pb、As与Cu、电导率与Pb等呈极显著正相关（p<0.01），pH与Pb呈极显著负相关（p<0.01），在MICP技术处理后，全碳、全氮、全钾、全磷及有机质和重金属元素含量间的相关性增强。综上所述基于生境微生物的MICP修复技术能够降低尾矿渣重金属元素含量，调节尾矿渣土体养分状况从而调节污染土体质量，降低由矿产开发导致的环境污染风险。同时，进一步确定不同颗粒尾矿渣达到修复治理水平所需菌剂用量，具有污染土质治理修复应用价值。

Abstract

In order to clarify the treatment effect of Microbial Induced Calcium Carbonate Precipitation (MICP) in the heavy metal tailings in highly wind erosion areas in arid and semi-arid region

this paper used Bacillus (Lysinibacillus fusiformis) as the research object

which was the carbonate-mineralizing bacteria-spindle lysine bacillus screened in the habitat soil of the study area. We added the bacillus fermented liquid and the cementing solution mixed by 1mol/L urea and calcium chloride in the tailings slag to carry out indoor simulation experiments. And we compared the varieties of basic physical and chemical properties (heavy metal element content

electrical conductivity and enzyme activity) in tailing slag with different size which were from slope top (fine particles:K1<5 mm)

slope middle (middle particles:5 mm15 mm) before and after MICP treatment. And according to the method of two factor analysis of variance and correlation comparative analysis

we revealed the effect of MICP remediation technology based on habitat microorganisms on the regulation of regional tailing slag pollutants and determined the dosage of bacterial agent in MICP technology for the remediation of tailing slag with different particle sizes. The results showed that the MICP treatment had a significant effect on the basic physical and chemical properties such as heavy metal content

electrical conductivity and enzyme activity in tailings slag. Under the MICP treatment

the arsenic (As) content of small particles (K1<5 mm) reduced by 21%

the mercury (Hg) of medium particles (5 mm15 mm) decreased by 22%. The pH in K1 and K3 reduced by 5% and K2 reduced by 4%. The total potassium (K) and urease in K1 significantly increased by 33% (p<0.05)

the total carbon in K2 increased obviously by 39% (p<0.05)

and the sucrase in K3 increased by 7% (p<0.05). The amount of microbial carbon and nitrogen significantly increased in K1

K2 and K3 (p<0.05). At the same time

Ni-Pb

As-Cu

electrical conductivity and Pb were significantly positively correlated (p<0.01) under MICPtreatment and pH-Pb was extremely significantly negatively correlated (p<0.01). And after MICP treatment

the correlations among total nitrogen

total potassium

total phosphorus

the contents of organic matter

heavy metal elements were enhanced.In summary

the MICP remediation technology based on habitat microorganisms could reduce the content of heavy metal elements amount in tailings slag

adjust the nutrient status of tailings slag soil

so as to regulate the quality of contaminated soil

and reduce the risk of environmental pollution caused by mineral resources development. At the same time

this study further determined the application dosage of bacterial agent for different particle tailings restoration to reach the remediation level

which has the better application value in the treatment and remediation of polluted soil.

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