胶质芽孢杆菌与苜蓿根瘤菌双接种对排土场紫花苜蓿生长和土壤性质的影响

孔涛; 任曦玥; 张宇航; 吕刚; 郑爽; 冯奥哲

doi:10.13870/j.cnki.stbcxb.2021.04.044

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胶质芽孢杆菌与苜蓿根瘤菌双接种对排土场紫花苜蓿生长和土壤性质的影响

更新时间：2025-03-12

- 胶质芽孢杆菌与苜蓿根瘤菌双接种对排土场紫花苜蓿生长和土壤性质的影响
- Effect of Dual Inoculant of
- 水土保持学报 2021年35卷第4期页码：321-326
- 作者机构：
  
  1. 辽宁工程技术大学环境科学与工程学院,辽宁,阜新,123000
  2. 辽宁工程技术大学应用技术与经济管理学院,辽宁,阜新,123000
- 作者简介：
  
  [ "孔涛(1981-),男,汉族,陕西渭南人,副教授,博士,硕士生导师,主要从事矿区生态修复研究。E-mail:kongtao2005@126.com" ]
- 基金信息：
  
  国家重点研发计划项目（2017YFC1503105）辽宁工程技术大学学科创新团队项目（LNTU20TD-24）
- DOI：10.13870/j.cnki.stbcxb.2021.04.044
  中图分类号： S144;S963.22
- 纸质出版：2021
- 稿件说明：
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孔涛, 任曦玥, 张宇航, 等. 胶质芽孢杆菌与苜蓿根瘤菌双接种对排土场紫花苜蓿生长和土壤性质的影响[J]. 水土保持学报, 2021,35(4):321-326.

KONG Tao, REN Xiyue, ZHANG Yuhang, et al. Effect of Dual Inoculant of[J]. 2021, 35(4): 321-326.
孔涛, 任曦玥, 张宇航, 等. 胶质芽孢杆菌与苜蓿根瘤菌双接种对排土场紫花苜蓿生长和土壤性质的影响[J]. 水土保持学报, 2021,35(4):321-326. DOI： 10.13870/j.cnki.stbcxb.2021.04.044.

KONG Tao, REN Xiyue, ZHANG Yuhang, et al. Effect of Dual Inoculant of[J]. 2021, 35(4): 321-326. DOI： 10.13870/j.cnki.stbcxb.2021.04.044.

摘要

为了明确胶质芽孢杆菌和苜蓿根瘤菌在矿区排土场复垦中的协同作用，以矿区排土场土壤作为基质进行盆栽试验，设置两菌单接种处理、双接种处理及对照，其中胶质芽孢杆菌和苜蓿根瘤菌用量分别为1.50%和0.25%，测定紫花苜蓿产量指标、叶片生理指标、土壤速效养分含量和土壤微生物指标。结果表明，接种胶质芽孢杆菌和苜蓿根瘤菌的各处理均能显著提高紫花苜蓿的产量指标，其中以双接种处理效果最优，将生物量和结瘤量分别比对照显著提高110.27%和124.32%；在生理指标方面，双接种处理效果低于单接种处理，未表现出协同作用；在土壤养分和微生物性质方面，单接种胶质芽孢杆菌仅显著提高土壤速效钾含量，单接种苜蓿根瘤菌对大部分指标均有显著效果，而双接种处理效果最佳，土壤碱解氮、速效钾含量比对照分别显著提高94.14%和84.55%，土壤微生物指标MBC含量、呼吸强度、蔗糖酶、脲酶、磷酸酶、过氧化氢酶活性比对照分别显著提高204.02%，65.86%，212.32%，91.87%，30.57%，51.87%。双接种处理对紫花苜蓿产量指标、养分和微生物指标表现出显著的协同作用。因此，1.50%用量胶质芽孢杆菌和0.25%用量苜蓿根瘤菌双接种能够有效提高排土场土壤的复垦效果，具有协同作用，可以作为矿区排土场复垦的高效方法。

Abstract

In order to clarify the synergistic effect of Paenibacillus mucilaginosus and Rhizobium meliloti on the reclamation of dumping in mining area

the soil of dumping in the mining area was used as the substrate for a pot experiment

and the single inoculation treatments of two bacteria

dual inoculation treatments and control were set up. The dosages of P. mucilaginosus and R. meliloti in treatments were 1.50% and 0.25%

respectively. The alfalfa yield

leaf physiological indexes

soil available nutrients contents

soil microbial indexes were determined. The results showed that inoculant of P. mucilaginosus and/or R. meliloti could improve the yield of alfalfa

and dual inoculation treatment was the highest and better than the control by 110.27% and 124.32% for biomass and nodules index

respectively. As for physiological indexes

the effect of dual inoculation treatment was lower than that of single inoculation treatments

and no synergistic effect was found. In terms of soil nutrients and microbial properties

single inoculation of P. mucilaginosus only significantly increased the soil available potassium content

and single inoculation of R. meliloti had a significant effect on most indicators. Dual inoculation treatment had the best effect. It increased significantly the contents of available nitrogen and potassium of soil by 94.14% and 84.55% respectively compared with the control. The soil microbial indexes

including MBC content

respiration intensity

invertase

urease

phosphatase

and catalase activities

were significantly increased by 204.02%

65.86%

212.32%

91.87%

30.57% and 51.87%

respectively. The dual inoculation treatment showed a significant synergistic effect on the alfalfa yield

soil nutrient and microbial indexes. Therefore

the dual inoculant of P. mucilaginosus at the dosage of 1.50% and R. meliloti at the dosage of 0.25% could effectively improve the soil reclamation effect of the dumping with a synergistic effect and could be used as an efficient method for the reclamation of the dumping in the mining area.

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