2种菌联合修复农田土壤镉污染的研究

刘悦畅; 李保珍; 王涛; 王兰

doi:10.13870/j.cnki.stbcxb.2020.04.052

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2种菌联合修复农田土壤镉污染的研究

更新时间：2025-03-12

- 2种菌联合修复农田土壤镉污染的研究
- Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution
- 水土保持学报 2020年34卷第4期页码：364-369
- 作者机构：
  
  山西大学生命科学学院,太原,030006
- 作者简介：
  
  [ "刘悦畅(1995-),女,硕士研究生,主要从事微生物的开发利用研究。E-mail:980168324@qq.com" ]
- 基金信息：
  
  山西省重点研发计划项目（201703D221008-3）
- DOI：10.13870/j.cnki.stbcxb.2020.04.052
  中图分类号：
- 纸质出版：2020
- 稿件说明：
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刘悦畅, 李保珍, 王涛, 等. 2种菌联合修复农田土壤镉污染的研究[J]. 水土保持学报, 2020,34(4):364-369.

LIU Yuechang, LI Baozhen, WANG Tao, et al. Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution[J]. 2020, 34(4): 364-369.
刘悦畅, 李保珍, 王涛, 等. 2种菌联合修复农田土壤镉污染的研究[J]. 水土保持学报, 2020,34(4):364-369. DOI： 10.13870/j.cnki.stbcxb.2020.04.052.

LIU Yuechang, LI Baozhen, WANG Tao, et al. Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution[J]. 2020, 34(4): 364-369. DOI： 10.13870/j.cnki.stbcxb.2020.04.052.

摘要

采用盆栽试验，运用BCR四步法、火焰原子吸收法和稀释涂布平板法等方法，研究了沼泽红假单胞菌（Rhodopseudanonas palustris）和枯草芽孢杆菌（Bacillus subtilis）联合作用对修复农田土壤重金属镉污染盆栽种植小白菜（Brassica campestris L.ssp.chinensis Makino）的效果及安全性，旨在探讨降低土壤镉生物有效性的有效途径，为新型生物菌肥研究提供理论依据。结果表明，沼泽红假单胞菌、枯草芽孢杆菌单独作用以及联合作用均能使农田土壤中镉的固定态增加，降低农田土壤中镉的生物有效性，联合菌处理组镉的生物有效性降低最显著，为32.70%，均对农田土壤中微生物各生理类群数量有显著性影响（P<0.05），其中，细菌的数量达到105 CFU/g；均显著降低了小白菜根、茎中镉含量，联合菌处理组根、茎中镉的去除率分别达到58.06%~71.37%和73.66%~76.70%；均增加了小白菜株高、茎鲜重、叶绿素a、b的含量和叶绿素a/b的比值，联合菌处理组小白菜的生物量增加18.13%~69.55%。研究结果说明，沼泽红假单胞菌和枯草芽孢杆菌联合作用，对农田土壤镉污染的修复效果显著，且促进了小白菜的生长，具有修复农田土壤重金属污染的潜力。

Abstract

This research used the potted plant approach

BCR four steps work

flame atomic absorption method

and the method of dilution coated tablet to study the joint action of Rhodopseudanonas palustris and Bacillus subtilis

as well as the efficacy and safety

for remediation of cadmium pollution in farmland soil by Chinese cabbage in order to explore its effectiveness and to provide theoretical basis for application of the new type bio-bacterial manure. The results showed that either R. palustris or B. subtilis alone

or their combination could enhance the fixation of the metal in soil by increasing insoluble state cadmium and decreasing cadmium bioavailability. The combination of two bacteria significantly reduced cadmium bioavailability by 32.70%. All bacteria treatments showed significant influence on the number of soil microorganisms (P<0.05)

and the amount of bacteria reached 105 CFU/g. The treatments with various microorganisms all increased the plant height and fresh weight of cabbage

the content of chlorophyll a and b

and the ratio of chlorophyll a/b

meanwhile reduced the content of cadmium in the root and stem of cabbage with removal rate of 58.06%~71.37% and 73.66%~76.70%

respectively. The combination treatment group had the highest remediating effect with the biomass of Chinese cabbage increased by 18.13%~69.55%. The results demonstrated that the combination of R. spalustris and B. subtilis had a strong ability to remove cadmium from contaminated soil

could significantly promote the growth of Chinese cabbage

and showed the potential for remediation of heavy metal pollution in farmland soil.

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