A kind of tetracyclines-oxytetracycline was used as target pollutant to analyze the potential influences of veterinary antibiotics pollution on maize growth. A potted maize experiment was conducted to study the influences of oxytetracycline-contained soils on the biomass

photosynthesis parameters and antioxidant enzymes during the growth stage of maize in the soils with different substrates: microbial inoculants(JF)

mushroom bran (JK)

wormcast (QY)

and biomass charcoal (S) etc. with the single and combined application. The results showed that oxytetracycline had a greater inhibiting effect on the roots of maize than on the aboveground. The length and dry weight of maize roots under the treatments of JK

microbial inoculants+wormcast (JQ)

microbial inoculants +biomass charcoal (JS) increased by 9.1%

9.4%

13.7% and 66.46%，183.4%，46.72% respectively than the OTC treatment. The addition of substrates reduced the inhibiting effect of Oxytetracycline on the net photosynthetic rate

stomatal conductance and transpiration rate of maize effectively. JK increased the chlorophyll contents in three stages of maize

the increases in seedling

jointing and maturation stages were 7.78%

33.33% and 12.03% respetively. The CAT and POD activities under JS increased by about 48% and 43.2% than the OTC. The addition of S

JQ and JK reduced the proline contents by 59%

16.5% and 55.4% respectively than the OTC in the seeding

jointing and maturation stages. In general

doxycycline had an obvious inhibiting effect on maize growth

but adding different substrates could alleviate the adverse effects on maize’s physiological traits. The addition of JK

JS

S）and JQ had significant positive effect on maize’s growth. These findings provided the theoretical basis for screening the substrates that could reduce antibiotics’ influences on maize’s growth

as well as for the assessment of oxytetracycline risk to crops.