In order to study the effects of bio-organic fertilizer on the fertility and enzymes activities in fluvo-aquic soil. A field experiment was conducted

and the changes of soil fertility characteristics and enzymes activities were measured in different growth stages of cucumber at 0—15 cm and 15—30 cm soil layers. The bio-organic fertilizer was once applied at rates of 0

10 and 20 t/hm2

designated as CK

Y1 and Y2 respectively. The results showed that: (1) In the same growth period of cucumber

all the contents of total nitrogen

ammonium nitrogen

nitrate nitrogen

available phosphorus and organic matter increased at 0—15 cm and 15—30 cm soil layers with increasing bio-organic fertilizer application rates. In the mature period of cucumber

Y1 increased the contents total nitrogen

ammonium nitrogen

nitrate nitrogen

available phosphorus and organic matter in soil by 5.99%

11.21%

17.48%

5.98% and 39.30% respectively

while the corresponding values were 6.59%

25.86%

21.99%

10.78% and 61.76% respectively in Y2

compared with CK. Significant differences were found in ammonium nitrogen and organic matter among the three treatments. Under the treatments applied with bio-organic fertilizer at 15—30 cm soil layer

the nitrate nitrogen contents were increased by18.83% to 23.27%

and available phosphorus by 39.30% to 61.76%. (2) In the same growth period

the increased trends of soil urease and catalase activities were found with the increasing bio-organic fertilizer application rates at different soil layers. In the mature period

under the treatments applied with bio-organic fertilizer

soil urease activity was increased by 47.02% to 62.08%

and catalase activity by 14.41% to 23.73% compared with CK at 0—15 cm soil layer

while the values were 49.17% to 67.75% and 40.01% to 63.33% at 15—30 cm soil layer. (3) With the growing season

soil fertility indexes and urease activity decreased

while catalase activity increased first and then decreased. Besides

soil fertility indexes and enzyme activities at 0—15 cm soil layer were significantly higher than those at 15—30 cm soil layer. In conclusion

the soil fertility

urease and catalase activities were obviously increased in treatments applied with bio-organic fertilizer

and the increases in soil fertility

urease and catalase activities were found in fluvo-aquic soil with the increasing bio-organic fertility application rates.