This paper studied the dynamics of soil aggregation in responses to organic materials and their incorporation methods through a laboratory cultivating experiments. Five treatments were involved: no addition as a control (CK)

mixing straw (M-Str)

straw mulching (Str)

biochar (BC) and swine manure (SM). The results show that the M-Str and SM treatments significantly increased soil organic carbon (SOC)

hot water extractable carbon (HWC)

tensile strength (TS) and mean weight diameter (MWD) of aggregate stability (p＜0.05)

whereas the Str treatment did not enhance SOC and MWD (p＞0.05). Compared to the CK treatment

the BC treatment did not improve MWD although it increased SOC significantly (p＜0.05). The aggregate stability in the M-Str and SM treatments was linearly related to soil respiration (CO2) (p＞0.05). Our results demonstrate that organic materials and their incorporation methods played a critical role in soil aggregation stability.