The experiment of using soil column methods which studied the effects of different fertilizers types on the temporal-spatial variations of soil enzyme activities and microorganism's number was conducted under the condition of equal nitrogen and three treatments were installed which included carbamide treatment

organic fertilizer treatment and mixed fertilizer treatment.The results showed that the effects of different fertilizers types on the temporal-spatial variations of soil enzyme activities and microorganism's number were obvious under the condition of soil column methods.The total urease and protease activities in different soil depth showed mixed fertilizer treatment >organic fertilizer treatment>carbamide treatment without exception;the total urease activities decreased with the increasing soil depth without exception

the urease activities in the soil layers of 0~20 cm and 0~40 cm respectively took possession of the total urease activities in the total soil of 1.2 m with 50% upwards and 79% during the whole growth stages of wheat

the proportion of 0~20 cm and 20~120 cm was approximately equal;the max value of urease activities in different soil layers occurred during jointing stage;the urease activities increase during latter growth stage in different soil depth.The max value of protease activities occurred during mature stage

in the next place during jointing stage

the peak value occurred during jointing and flowering stages

the off-peak value occurred during booting and active grain filling stages

the min value occurred during active grain filling stage in different soil layers under three different fertilizers types treatment;the peak value of soil protease activities occurred in the soil layer of 20~40 cm and 80~100 cm

protease activities in the soil layer of 20~40 cm were higher than 80~100 cm.The number of microorganisms showed mixed fertilizer treatment > organic fertilizer treatment>carbamide treatment without exception.The max number of bacteria occurred during over-winter stage

the max number of fungi occurred during booting stage in the same soil layer.The number of actinomyces increased with the advance of growth stages

the max value occurred during mature stage in the same soil layer.The max number of bacteria

fungi and actinomycets occurred in the soil layer of 20~40 cm

in the next place in the soil layer of 0~20 cm

the number decreased with the increasing depth of 40~120 cm soil layer during the different growth stages of winter wheat.