纸质出版:2007
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干旱和复水条件下转基因甘薯的光合特性[J]. 水土保持学报, 2007,(4):193-196.
Photosynthetic System Characteristics of Transgenic Sweet Potato under Water Stress and Rewatering[J]. 2007, (4): 193-196.
以转铜锌超氧化物歧化酶(Cu/Zn SOD)和抗坏血酸过氧化物酶(APX)基因甘薯(TS)及未转基因甘薯(NT)为实验材料
研究在旱后复水条件下转基因甘薯及未转基因甘薯抗氧化酶活性和光合特性变化.结果显示
连续36 h胁迫条件下
TS和NT的SOD活性都先降低后升高
但TS的SOD活性始终高于NT.胁迫至24 h时
TS的SOD活性约为NT的1.2倍
复水后二者SOD活性都下降.持续胁迫
TS的APX活性先升高后降低
NT与之相反
复水后TS和NT的APX活性都是先升高后降低
复水12 h
TS的APX活性是NT的1.5倍.水分胁迫条件下TS的膜质受伤害程度要轻于NT
胁迫24 h
复水12 h
NT的MDA含量均约为TS的1.2倍.胁迫12 h
TS和NT净光合速率都下降
继续胁迫
TS净光合速率开始上升
NT几乎保持不变
胁迫36 h
TS的净光合速率约为NT的1.5倍.复水后二者净光合速率都开始上升
复水12 h
TS净光合速率约为NT的3倍.胁迫时TS、NT胞间CO2浓度(Gi.)都逐渐增大
胁迫36 h时NT胞间CO2浓度显著高于TS
是其1.4倍.实验结果表明
同时转入SOD、APX抗氧化基因后
在胁迫及复水条件下转基因甘薯的抗氧化系统可以更好地保护及修复光合机制.
Two strains
Cu/Zn SOD and APX gene transferred sweet potato(TS) and non-transgenic sweet potato(Ipomoea batatas L.)(NT)
were used as experimental materials to study the effects of water stress and rewatering on photosynthetic characteristics of sweet potatoes.The result showed that the activities of SOD of both TS and NT first increased and then decreased under water stress
but the activity of SOD of TS was always higher than that of NT
under 24 h water stress
the activity of SOD of TS was 20% higher than that of NT.The activities of SOD of TS and NT both decreased after rewatering.Under water stress
the activity of APX of TS firstly increased and then decreased;in NT it firstly decreased and then increased.After rewatering
the activity of APX of both TS and NT firstly increased and then decreased
after 12 h rewatering
the activity of APX of TS was 50% higher than it of NT.Under water stress
the membrane structural stability of TS was better than it of NT
after 24 h water stress and 12 h rewatering
the MDA content of NT were 20% more than it of TS. At the beginning of the water stress
pn decreased in both TS and NT
when stress time became longer
pn in TS increased
in NT pn almost keep the same level
after 36 h stress
pn in TS is 50% higher than it in NT
after rewatering
pn in both TS and NT began to increased
after 12 h rewatering
pn in TS was 1 times higher than it in NT.Under water stress
Ci increased in both TS and NT.After 36 h water stress
Ci in NT was 40% higher than it in TS
after rewatering
Ci in both TS and NT decreased.The result showed that transferred both Cu/Zn SOD and APX gene
the antioxidative system of the transgenic sweet potato had a higher ability to protect and recover the photosynthetic system of the plant.
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