Extracellular Enzyme Stoichiometric Characteristics of Subtropical Forest Soil under Forest Fire Disturbance

GUO Qiling; WANG Jianqing; MAO Jiaoyan; SUN Jiawen; HUANG Yuqing; SHI Xiuzhen

doi:10.13870/j.cnki.stbcxb.2025.01.001

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Extracellular Enzyme Stoichiometric Characteristics of Subtropical Forest Soil under Forest Fire Disturbance

更新时间：2025-03-14

- Extracellular Enzyme Stoichiometric Characteristics of Subtropical Forest Soil under Forest Fire Disturbance
- Vol. 39, Issue 1, Pages: 94-101(2025)
- 作者机构：
  
  1. 福建师范大学地理研究所,福州,350117
  2. 福建师范大学地理科学学院碳中和未来技术学院,福州,350117
  3. 福建师范大学湿润亚热带生态-地理过程教育部重点实验室,福州,350117
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2025.01.001
  CLC： S714.3
- Published：2025
- 稿件说明：
移动端阅览
GUO Qiling, WANG Jianqing, MAO Jiaoyan, et al. Extracellular Enzyme Stoichiometric Characteristics of Subtropical Forest Soil under Forest Fire Disturbance[J]. 2025, 39(1): 94-101.
DOI：

GUO Qiling, WANG Jianqing, MAO Jiaoyan, et al. Extracellular Enzyme Stoichiometric Characteristics of Subtropical Forest Soil under Forest Fire Disturbance[J]. 2025, 39(1): 94-101. DOI： 10.13870/j.cnki.stbcxb.2025.01.001.

摘要

目的森林火灾作为亚热带森林生态系统主要的自然和人为干扰过程，严重影响土壤生物化学循环过程。为明确土壤胞外酶活性计量特征对林火干扰后恢复过程的响应。方法以亚热带次生林林火干扰后不同恢复阶段的土壤为研究对象，采用空间替代时间序列的方法选取7个次生林恢复阶段的亚热带森林土壤，并将其分为老龄林(60~70 a)、中龄林(30~40 a)和幼龄林(＜20 a)，通过测定土壤碳(C)、氮(N)、磷(P)相关的转化酶活性，揭示林火干扰下亚热带森林土壤胞外酶活性及其化学计量特征的演替规律。结果土壤纤维素水解酶(CBH)和β-N-乙酰氨基葡萄糖苷酶(NAG)活性随演替时间显著增加，其他酶活性无显著增加。相关性分析和冗余分析发现，土壤C、N质量分数是影响土壤胞外酶活性变化的关键因子。此外，幼龄林、中龄林和老龄林土壤胞外酶C、N、P计量比分别为1∶0.94∶1.53、1∶1.02∶1.63、1∶0.99∶1.47，表明亚热带地区森林土壤微生物生长受到P限制，且随着林火干扰后恢复年限增加逐渐改善。结论林火干扰后森林土壤N转化相关胞外酶活性随恢复时间呈递增趋势，土壤胞外酶化学计量逐渐趋于稳定，表明亚热带森林土壤健康状况随着林火干扰恢复过程逐渐改善。

Abstract

ObjectiveForest fire

as the primary natural and human-induced disturbance in subtropical forests

seriously affects the soil biochemical cycle. We aimed to clarify the response of soil extracellular enzyme activities to the restoration process following forest fire disturbance.MethodsWe investigated the soils of subtropical secondary forests at different stages of recovery following forest fire disturbances. Subtropical forest soils at seven stages of restoration were analyzed using a spatial substitution time series approach. The forests were categorized into three age groups

including old forests (60—70 years)

middle-aged forests (30—40 years)

and young forests (< 20 years). We aimed to reveal the succession patterns of extracellular enzyme activities and their stoichiometric characteristics following forest fire disturbances by measuring invertase activities related to soil carbon (C)

nitrogen (N)

and phosphorus (P) in subtropical forest soils.ResultsThe activities of soil cellulose hydrolase (CBH) and β-N-acetylglucosaminidase (NAG) increased significantly with forest stand age

but the activities of other extracellular enzymes did not increase significantly with stand age. Correlation analysis and redundancy analysis showed that soil C and N contents were the key factors affecting the change of soil extracellular enzyme activities. In addition

the ratios of soil extracellular enzyme C

N and P were 1∶0.94∶1.53

1∶1.02∶1.63 and 1∶0.99∶1.47 in young

middle and old forests

respectively

indicating that soil microorganisms in subtropical forest was limited by P

but such P-limitation was improved gradually with forest stand age following forest fire disturbance.ConclusionThe extracellular enzyme activities related to soil N transformation increased with forest stand age following forest fire disturbances in subtropical forest soils. The stoichiometric characteristics of soil extracellular enzyme gradually tended to be stable

indicating that soil health was progressively improved during the restoration process following forest fire disturbance in subtropical forests.

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