Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon

ZHANG Yating; LOU Xueyi; WU Jiarui; LIU Hongyuan; YE Binyu; XIAO Jiamin; LI Jiameng; MIAO Yining; CHEN Xinyan; LIU Yilin; TAN Yuyu; WANG Zhichao; LIU Peng

doi:10.13870/j.cnki.stbcxb.2024.06.039

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Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon

更新时间：2025-02-18

- Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon
- Vol. 38, Issue 6, Pages: 352-362(2024)
- 作者机构：
  
  浙江师范大学生命科学学院植物学实验室,浙江,金华,321004
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2024.06.039
  CLC： S154.39
- Published：2024
- 稿件说明：
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ZHANG Yating, LOU Xueyi, WU Jiarui, et al. Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon[J]. 2024, 38(6): 352-362.
DOI：

ZHANG Yating, LOU Xueyi, WU Jiarui, et al. Combined Effects of Humic Acid-Modified Magnetic Biochar and AMF on the Properties of Saline-Alkali Soil and Photosynthetic Parameters of Muskmelon[J]. 2024, 38(6): 352-362. DOI： 10.13870/j.cnki.stbcxb.2024.06.039.

摘要

[目的] 为改良腐殖酸—磁性生物炭(HA—MBC)

并探究改良后的HA—MBC与丛枝菌根真菌(AMF)联合作用对盐碱土性质与甜瓜生长的影响。[方法] 通过响应面优化法

分析磁性生物炭与腐殖酸配比(1∶4

1∶6

1∶8)、FeCl3、FeSO4试剂浓度(0.02

0.04

0.06 g/mL)及交联时长(48

96 h)对HA—MBC的影响

获取适合与AMF联施的最优改良方案。并通过盆栽试验

探究单菌、单炭和炭菌联作(AMF+10%B、AMF+20%B)对盐土、碱土理化性质

酶活性和甜瓜生长、光合指标的影响。[结果] 15

75 mmol/L盐碱胁迫时

20 g/kg AMF与20%HA—MBC共同施用缓解效果最优

二者"协同增效"。一方面

HA—MBC负载的铁氧化合物及HA通过增加表面官能团及孔隙率

改善土壤理化性质

而AMF能通过形成共生菌根

二次富集有机质、营养元素

并提升土壤酶活性

进而增加甜瓜株高根长

促进Fv/Fm的回升与F0、qN的降低;另一方面

HA—MBC的施用能够使盐土、碱土中AMF侵染率分别提高19.46%

22.72%

进一步提高AMF作用效果。同时

Fe2+、Mg2+、N等营养元素在炭菌间能形成转化循环

提高养分利用率。150 mmol/L盐碱胁迫时

施加低剂量HA—MBC仍起改良土壤、缓解胁迫的作用

而高剂量炭施加则对指标的改善起抑制作用。可能是过施HA—MBC时

表面官能团过度聚集有毒物质、盐离子并竞争营养元素

使AMF侵染率降低

削弱共生菌根现象

进而降低甜瓜光合指标。[结论] 在添加0.04 mmol/L FeCl3、FeSO4试剂

生物炭与腐殖酸配比6∶1

交联时间72 h下制备的HA—MBC适合与AMF共同施用。二者联作在低、中浓度盐碱胁迫水平下可改善盐碱土理化性质和养分结构

促进甜瓜光合作用

而高浓度胁迫下过施HA—MBC抑制土壤指标和甜瓜光合指标的回升。

Abstract

[Objective] To improve humic acid-modified magnetic biochar (HA-MBC) and explore the combined effects of modified biochar and arbuscular mycorrhizal fungi (AMF) on the properties of saline-alkali soils and the growth of muskmelon. [Methods] Through response surface methodology

the effects of the ratio of magnetic biochar to humic acid (1∶4

1∶6

1∶8)

the concentration of FeCl3 and FeSO4 reagents (0.02

0.04

0.06 g/mL)

and the cross-linking duration (48

96 h) on HA-MBC were analyzed

to procure the optimum amendment scheme suitable for combined application with AMF. Moreover

by conducting pot experiments

the influence of individual fungi

individual biochar

and combined biochar-fungi (AMF+10%B

AMF+20%B) on the physicochemical properties

and enzyme activities of saline soil and alkaline soil

and the photosynthetic parameters of muskmelon were studied. [Results] Under 15 and 75 mmol/L saline-alkali stress

combined application of 20 g/kg AMF with 20% HA-MBC showed the best mitigation effects

with a "synergistic enhancement" observed. On one hand

the iron oxides and humic acids loaded on HA-MBC improved the physical and chemical properties of soil by increasing the quantity of surface functional groups and porosity

while AMF formed symbiotic roots to further enrich organic matter and nutrients

and increase soil enzyme activities

thus increasing the height and root length of muskmelon and promoting the recovery of Fv/Fm and the reduction of F0 and qN. On the other hand

the application of HA-MBC could increase the colonization rate of AMF in saline and alkaline soils by 19.46% and 22.72%

respectively

thus further enhancing the effects of AMF. Moreover

nutrients such as Fe2+

Mg2+ and nitrogen could form a transformation cycle between biochar and fungi

thus improving nutrient use efficiency. Under 150 mmol/L saline-alkali stress

applying a low dose of HA-MBC still played a role in ameliorating soil and mitigating stress

while applying high doses of biochar inhibited the improvement of indicators. This might be due to excessive application of HA-MBC causing surface functional groups to aggregate toxic substances and salt ions excessively and compete for nutrients

subsequently reducing AMF colonization rates

weakening the mycorrhizal symbiosis

and hence diminishing the photosynthetic parameters of muskmelon. [Conclusion] HA-MBC prepared with a biochar to humic acid ratio of 6∶1 and cross-linking time of 72 h

using 0.04 mmol/L FeCl3 and FeSO4 reagents

is suitable for combined application with AMF. The synergistic application of HA-MBC and AMF can improve the physicochemical properties and nutrient structure of saline-alkali soil and promote the photosynthesis of muskmelon under low to medium saline-alkali stress levels

whereas excessive application of HA-MBC under high stress concentrations inhibits the improvement of soil indicators and the recovery of photosynthetic parameters in muskmelon.

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