Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of

SONG Chenggong; WANG Keqin; SONG Yali; LI Xiaodong; ZHANG Jiguo; LI Yun; DENG Qiujiang

doi:10.13870/j.cnki.stbcxb.2024.06.009

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Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of

更新时间：2025-02-18

- Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of
- Vol. 38, Issue 6, Pages: 273-283(2024)
- 作者机构：
  
  1. 西南林业大学生态与环境学院,昆明,650224
  2. 国家林业和草原局云南玉溪森林生态系统国家定位观测研究站,云南,玉溪,653100
  3. 禄劝彝族苗族自治县林业和草原局, 云南禄劝,651500
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2024.06.009
  CLC： S718.54
- Published：2024
- 稿件说明：
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SONG Chenggong, WANG Keqin, SONG Yali, et al. Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of[J]. 2024, 38(6): 273-283.
DOI：

SONG Chenggong, WANG Keqin, SONG Yali, et al. Response of Microbial Community Structure and Diversity to Nitrogen Deposition in Soils of[J]. 2024, 38(6): 273-283. DOI： 10.13870/j.cnki.stbcxb.2024.06.009.

摘要

[目的] 探究氮沉降下滇中高原云南松土壤化学性质及土壤微生物群落多样性和结构组成的变化特征

并分析土壤微生物与土壤化学性质的相关关系

为了解氮沉降下森林土壤生态系统养分循环变化趋势及观测土壤微生态变化提供科学依据。[方法] 选取滇中高原云南松林土壤为研究对象

以CO(NH2)2为N源

设置对照CK [N 0 g/(m2·a)]、低氮LN [N 10 g/(m2·a)]、中氮MN [N 20 g/(m2·a)]和高氮HN [N 25 g/(m2·a)]4个不同梯度进行模拟N沉降试验

测定分析土壤细菌、真菌群落结构及多样性和土壤pH、有机碳(TOC)、全氮(TN)、全磷(TP)、铵态氮(NH+4-N)和硝态氮(NO-3-N)的变化特征。[结果] (1)在不同季节下

与CK相比各氮处理下土壤pH、TOC含量均降低

TN均升高

NH+4-N和NO-3-N均在MN、HN处升高;(2)细菌和真菌α多样性雨季均高于旱季;与CK相较细菌α多样性在2个季节下LN处上升

而真菌α多样性则在旱季LN处上升;(3)在旱季和雨季细菌优势菌门均为变形菌门、酸杆菌门和放线菌门

真菌优势菌门为担子菌门、子囊菌门和被孢菌门;(4)在旱季和雨季

细菌和真菌优势菌属与pH、TOC、TN、NO-3-N呈显著相关关系。[结论] 氮沉降可以为土壤微生物提供NH+4-N和NO-3-N等有效养分

但由于氮沉降导致土壤酸化使土壤TOC含量降低

影响土壤微生物群落结构。

Abstract

[Objective] To study the characteristics of changes in chemical properties and diversity and structural composition of soil microbial communities in Pinus yun-nanensis soils on the Central Yunnan Plateau under nitrogen deposition

and to analyze the correlation between soil microorganisms and soil chemical properties

in order to provide a scientific basis for understanding the trend of changes in nutrient cycling in forest soil ecosystems under nitrogen deposition and observing soil microbiological changes. [Method] The soil of P. yunnanensis forests on the Central Yunnan Plateau was selected as the research object

CO(NH2)2 was used as the N source

and four different gradients were set up for simulated N deposition experiments

including control CK [N 0 g/(m2·a)]

low nitrogen LN [N 10 g/(m2·a)]

medium nitrogen MN [N 20 g/(m2·a)]

and high nitrogen HN [N 25 g/(m2·a)]

the structure and diversity of soil bacterial and fungal community were analyzed

and the characteristics of the changes of soil pH

TOC

NH+4-N and NO-3-N were determined. [Results] (1) In both the dry and rainy seasons

soil pH and TOC content were reduced

and TN was increased under each N treatment when compared with CK

and both NH+4-N and NO-3-N were increased at MN and HN compared to CK; (2) Both bacterial and fungal α-diversity were higher in the rainy season than in the dry season; bacterial α-diversity increased at LN under both seasons compared to CK

whereas fungi α-diversity increased at LN in the dry season; (3) In both the dry and rainy seasons

the dominant bacterial phyla were Proteobacteria

Acidobacteria and Actinobacteria

and the dominant fungal phyla were Basidiomycota

Ascomycota and Mortierellomycota; (4) The dominant bacterial and fungal genera were significantly correlated with pH

TOC

TN and NO-3-N during the dry and wet seasons. [Conclusion] Nitrogen deposition can provide soil microorganisms with effective nutrients such as NH+4-N and NO-3-N

but soil acidification due to nitrogen deposition can reduce soil TOC content and affect the structure of soil microbial communities.

关键词

Keywords

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