Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of

MAO Xuanwen; LU Jinhao; RUAN Xinyi; BAI Xiaowen; LIU Hongyuan; ZHAO Hang; LIN Ya'nan; JIANG Shirui; SONG Yang; ZHANG Baoxin; TAN Yuyu; LIU Peng

doi:10.13870/j.cnki.stbcxb.2024.01.037

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Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of

更新时间：2025-03-12

- Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of
- Vol. 38, Issue 1, Pages: 176-186(2024)
- 作者机构：
  
  浙江师范大学生命科学学院植物学实验室,浙江,金华,321004
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2024.01.037
  CLC： Q945.78
- Published：2024
- 稿件说明：
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MAO Xuanwen, LU Jinhao, RUAN Xinyi, et al. Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of[J]. 2024, 38(1): 176-186.
DOI：

MAO Xuanwen, LU Jinhao, RUAN Xinyi, et al. Effects of Low-Temperature Stress on Chlorophyll Fluorescence Parameters and Antioxidant System of[J]. 2024, 38(1): 176-186. DOI： 10.13870/j.cnki.stbcxb.2024.01.037.

摘要

[目的] 为探明气候变化背景下低温对三角梅生理生化表现及其对低温胁迫的内在适应机制。[方法] 以具有强、中、弱耐寒能力的宫粉、热火、百变三角梅为材料

采用室内盆栽试验

低温处理7

21天

研究不同耐寒型三角梅光合系统和抗氧化体系在各周期下对低温胁迫的响应情况。[结果] (1)低温处理期间

各品种初始荧光(F0)均有所上升

其中宫粉三角梅变化最为显著

较第0天增长60.2%

最大荧光(Fm)、PSII有效光化学电子产量(Fv/Fm)以及光合相对电子传递速率(ETR)则迅速降低

并于第21天时达到最低水平。三角梅的光化学猝灭系数(qP)均随着低温胁迫加剧而下跌

而热火三角梅与宫粉三角梅的非光化学猝灭系数(qN)大幅提高。(2)低温胁迫后各品种三角梅SOD、POD、CAT酶活性均显著下降

并随着胁迫时间的延长而加剧

即低温对三角梅抗氧化酶活性产生严重抑制

其中宫粉三角梅的SOD、POD、CAT酶活性均高于其余2个品种

可见宫粉具有强抗寒能力。(3)在AsA-GSH循环体系中

低温下各三角梅叶AsA含量与DHA含量均明显增加

21天时3个品种三角梅的GR活性分别较0天上升180.91%

175.97%

112.37%

且宫粉三角梅在21天时AsA/DHA比率、APX活性与DHAR活性显著提升。[结论] 低温通过影响PSⅡ光化学反应中心而抑制植株的生长

三角梅可通过开启光合系统保护机制减少光抑制

此外还积累渗透调节物质

启动抗氧化系统产生应激调节作用

以清除自身体内快速增生的ROS减轻低温伤害。

Abstract

[Objective] This study is aimed to explore the physiological and biochemical manifestations of Bougainvillea spectabilis Willd. to low temperature and the intrinsic adaptation mechanism to cold stress in the context of climate change. [Methods] Taking Bougainvillea Paederia Foetida

Bougainvillea Flaming Sumba and Bougainvillea Duranta with strong

medium

and weak cold tolerance as test materials

through indoor pot experiments

we conducted low temperature treatments for 7

14 and 21 days and studied the responses of the photosynthetic and antioxidant systems of different cold tolerant Bougainvillea to cold stress during these different periods. [Results] (1) During the low-temperature treatment period

the initial fluorescence (F0) of all varieties increased

with the most significant change in B. Paederia Foetida

which increased by 60.2% compared to day 0. Maximum fluorescence (Fm)

PSII effective photochemical electron production (Fv/Fm)

and photosynthetic relative electron transfer rate (ETR) rapidly decreased and reached their lowest levels at day 21. As the intensification of low temperature stress

the photochemical quenching coefficient (qP) of the three Bougainvillea types decreased

while the non-photochemical quenching coefficient (qN) of B. Flaming Sumba and B. Paederia Foetida increased significantly. (2) After exposure to low temperature stress

the activities of SOD

POD and CAT enzymes in each variety decreased significantly and intensified with the extension of stress time

indicating that low temperature seriously inhibited the antioxidant enzyme activity of Bougainvillea. Among them

the SOD

POD and CAT enzyme activities of B. Paederia Foetida were higher than those of the other two varieties

indicating that B. Paederia Foetida had strong cold resistance. (3) In the AsA-GSH cycle system

the content of AsA and DHA of each Bougainvillea leaf significantly increased under low temperature. Compared to day 0

the GR activity of the three Bougainvillea types at day 21 increased by 180.91%

175.97% and 112.37%

respectively. Additionally

the AsA/DHA ratio

APX activity and DHAR activity of B. Paederia Foetida significantly increased at day 21. [Conclusion] Low temperature inhibited plant growth through affecting the PSII photochemical reaction center. However

Bougainvillea could activate their photosynthetic system protection mechanism to reduce photoinhibition. Furthermore

it could accumulate osmoregulation substances

activate the antioxidant system

and exert stress regulatory effects to eliminate rapidly proliferating ROS in its own body and alleviate low-temperature damage.

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