Effects of Phenolic Acidification on Physiological Characteristics and Rhizosphere Microecology of

GE Jieke; YE Yumeng; LOU Xueyi; ZHANG Ruqi; YANG Ziwen; WANG Yueyi; HUANG Xu; JIN Hua; YANG Yanpei; AI Jinxiang; MA Mengwen; WU Yuhuan; LIU Peng

doi:10.13870/j.cnki.stbcxb.2023.03.033

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Effects of Phenolic Acidification on Physiological Characteristics and Rhizosphere Microecology of

更新时间：2025-03-12

- Effects of Phenolic Acidification on Physiological Characteristics and Rhizosphere Microecology of
- Vol. 37, Issue 3, Pages: 258-266(2023)
- 作者机构：
  
  1. 浙江师范大学化学与生命科学学院,浙江,金华,321004
  2. 杭州师范大学生命与环境科学学院,杭州,310036
  3. 中国科学院沈阳应用生态研究所,沈阳,110016
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2023.03.033
  CLC： S567.2
- Published：2023
- 稿件说明：
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GE Jieke, YE Yumeng, LOU Xueyi, et al. Effects of Phenolic Acidification on Physiological Characteristics and Rhizosphere Microecology of[J]. 2023, 37(3): 258-266.
DOI：

GE Jieke, YE Yumeng, LOU Xueyi, et al. Effects of Phenolic Acidification on Physiological Characteristics and Rhizosphere Microecology of[J]. 2023, 37(3): 258-266. DOI： 10.13870/j.cnki.stbcxb.2023.03.033.

摘要

酚酸类物质对植物产生明显化感作用

威胁植物生长和加工产品质量。以栝楼(Trichosanthes kirilowii Maxim.)为研究对象

通过室内盆栽试验

在土壤中添加3种不同浓度(0

100

150

200 mg/kg

分别为CK、T1、T2、T3、T4)的对羟基苯甲酸(pHBA)、苯甲酸(BA)、肉桂酸(CA)处理液

研究酚酸对栝楼生理和土壤特性的影响

阐明酚酸化感作用的强度和规律。结果表明:(1)T1浓度pHBA能够促进栝楼根系伸长

根鲜重增长率可达19.25%;BA影响栝楼生物量的积累

T4浓度下可抑制鲜重和根鲜重至CK的35.17%和58.38%;T4浓度下CA对株高和根长的抑制效果最为明显

最大降幅达到66.19%和63.13%。(2)高浓度pHBA能降低根系活力

最大抑制率为33.55%;BA对根系活力的化感效应与浓度呈正相关

最大抑制率为47.41%。3种酚酸对蒸腾速率的作用效果略有不同

但高浓度下均表现为抑制作用。(3)pHBA与CA提高SOD、POD活性

下调CAT活性;BA使SOD、POD、CAT活性都被抑制

分别降低47.42%

59.07%

45.43%; 3种酚酸胁迫下栝楼膜脂过氧化程度都加剧

但随胁迫时间延长

其损伤得到缓解。(4)酚酸胁迫打破土壤微生物结构平衡

土壤中脲酶与蔗糖酶活性对其表现更为敏感

并促使微生物结构从"细菌型"向"真菌型"转变。3种酚酸相较之下

苯甲酸对栝楼的化感效应程度最深

对羟基苯甲酸次之

肉桂酸最弱。降低土壤中酚酸含量有利于缓解栝楼种植过程的化感效应

增强栝楼的生存能力

进而提升栝楼产量与品质。

Abstract

Phenolic acids can have a significant allelopathic effect on plants

threaten plant growth and processing product quality. Trichosanthes kirilowii Maxim. was used as the study object. Through the indoor pot experiment

five different concentrations (0

100

150

200 mg/kg

designated as CK

T4) of p-Hydroxybenzoic acid (pHBA)

Benzoic acid (BA)

and Cinnamic acid (CA) treatment solutions were added to the soil to study their effects on the physiology and soil characteristics of T. kirilowii

and the intensity and law of the allelopathic effect of phenolic acid were elucidated. The results showed that: (1) pHBA at T1 concentration can promote the elongation of the root system of T. kirilowii

and the growth rate of root fresh weight can reach 19.25%; BA affects the biomass accumulation of T. kirilowii

and at T4 concentration

it can inhibit the fresh weight and root fresh weight to 35.17% and 58.38% of the control group; under T4 concentration

CA have the most obvious inhibitory effect on plant height and root length

with the largest decrease of 66.19% and 63.13%. (2) Low concentration of pHBA could enhance root vigor

while high concentration inhibited it

the maximum inhibition rate was 33.55%; the allelopathic effect of BA on root vigor was positively correlated with the concentration

and the maximum inhibition rate was 47.41%. The effect of phenolic acid on transpiration rate and root vigor were slightly different

but the treatment of 150-200 mg/L showed obvious allelopathic inhibitory effect. (3) pHBA and CA improved SOD and POD activity and downregulated CAT activity; BA inhibited the activity of SOD

POD and CAT

which decreased by 47.42%

59.07% and 45.43%

respectively. The degree of lipid peroxidation of the T. kirilowii membrane increased under all three phenolic acid stresses

but the damage was relieved as the stress time prolonged. (4) Phenolic acid stress significantly changed the soil microecology

and the activities of urease and invertase in the soil were more sensitive to it

and the microbial structure changed from "bacterial" to "fungal". The same concentration of benzoic acid had the deepest allelopathic effect on T. kirilowii cultivation

followed by p-hydroxybenzoic acid and the weakest was cinnamic acid. Therefore

reduction of the phenolic acid content in the soil is conducive to alleviate the allelopathic effect of the T. kirilowii

enhance the viability of the T. kirilowii

and thus improve the yield and quality of the T. kirilowii.

关键词

Keywords

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