Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil

WANG Hongping; BU Lianyan; CHEN Wenfeng; AN Derong; ZHANG Yinglong; WEI Gehong; WANG Honglei

doi:10.13870/j.cnki.stbcxb.2023.01.044

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Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil

更新时间：2025-03-12

- Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil
- Vol. 37, Issue 1, Pages: 345-353(2023)
- 作者机构：
  
  1. 西北农林科技大学水土保持研究所, 陕西杨凌,712100
  2. 西北农林科技大学生命科学学院, 旱区作物逆境生物学国家重点实验室, 陕西农业与环境微生物重点实验室, 陕西杨凌,712100
  3. 中国农业大学生物学院,北京,100193
  4. 西北农林科技大学植物保护学院, 陕西杨凌,712100
  5. 陕西省神木市生态保护建设协会, 陕西神木,719399
  6. 中国科学院水利部水土保持研究所, 陕西杨凌,712100
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2023.01.044
  CLC： S154.3
- Published：2023
- 稿件说明：
移动端阅览
WANG Hongping, BU Lianyan, CHEN Wenfeng, et al. Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil[J]. 2023, 37(1): 345-353.
DOI：

WANG Hongping, BU Lianyan, CHEN Wenfeng, et al. Effects of Combined Application of Functional Microbial Agents and Biochar on Sandy Soil[J]. 2023, 37(1): 345-353. DOI： 10.13870/j.cnki.stbcxb.2023.01.044.

摘要

研究生物炭和功能菌剂协同调控沙化土壤养分和微生物多样性与功能

可为沙化土壤肥力培育提供理论依据。选择毛乌素沙地典型生态经济林沙化土壤为研究对象

采用田间定位试验

运用高通量扩增测序技术

解析不同添加量(2%

8%)生物炭处理及其配施定量功能菌剂(枯草芽孢杆菌+巨大芽孢杆菌+胶质芽孢杆菌)处理对土壤化学性质及细菌菌群多样性与功能的影响。结果表明:(1)与单一生物炭处理相比

生物炭+功能菌剂处理组土壤有效氮、磷养分含量分别提升44.71%和187.36%;(2)生物炭单施或者配施功能菌剂均使酸杆菌门相对丰度显著降低

而生物炭+功能菌剂处理中厚壁菌门的相对丰度

分别增加163.80%

155.15%

100.21%

并且不同程度上调土壤细菌物种丰富度和多样性;(3)功能菌剂与生物炭配施改变土壤细菌介导的碳氮循环功能

如显著提高土壤细菌的有机物质分解功能

壳聚糖和木聚糖分解过程较对照组分别上升186.54%

242.46%

增强细菌的氨化和呼吸功能

而单施生物炭处理提升细菌的反硝化与固氮功能。综上

生物炭和功能菌剂的添加提高土壤有效氮磷养分含量

显著改变土壤细菌群落多样性与群落功能

生物炭配施功能菌剂优于单施生物炭处理

该结果可为沙化土壤改良方法的选择提供科学依据和理论参考。

Abstract

Investigating the biochar and functional fungal agents to coordinate the regulation of desertified soil nutrients and microbial diversity and function can provide a theoretical basis for the fertility cultivation of desertified soil. The soil of the typical ecological and economic forest of the Mu Wusu Sandy Land was selected as the research object

and the effects of different amounts (2%

8%) of biochar treatment and its combined application of quantitative functional agent treatment (Bacillus subtilis + Bacillus megaterium + Bacillus colloid) on soil chemical properties

bacterial diversity and function were analyzed by field positioning experiment and high-throughput amplification and sequencing technology. The results showed that: (1) Compared with the single biochar treatment

the soil available nitrogen and phosphorus nutrient contents of the biochar + functional agent treatment group increased by 44.71% and 187.36%

respectively. (2) The relative abundance of the Acidobacteria was significantly reduced by the single application or combination of functional agents of biochar

while the relative abundance of the phylum Firmcutes in the treatment of biochar + functional bacteria increased by 163.80%

155.15%

and 100.21%

respectively

and the richness and diversity of soil bacterial species were increased to varying degrees. (3) The combined application of functional agents and biochar changed the carbon and nitrogen cycle function mediated by soil bacteria

such as significantly improving the decomposition function of organic matter of soil bacteria

and the decomposition process of chitosan and xylan increased by 186.54% and 242.46% respectively compared with the control group; enhanced the ammonization and respiratory function of bacteria; while single biochar treatment improved the denitrification and nitrogen fixation function of the bacteria. In summary

biochar with functional bacterial agents is better than single application of biochar treatment. Biochar and functional bacteria agents added to improve the soil available nitrogen and phosphorus nutrient content

significantly changed the soil bacterial community diversity and community function. These results can provide a scientific basis and theoretical reference for the selection of desertification soil improvement methods.

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