Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet

CAO Lihua; LIU Heman; YANG Hong; LIAN Yuzhen

doi:10.13870/j.cnki.stbcxb.2022.06.045

您当前的位置：

首页 >

文章列表页 >

Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet

更新时间：2025-03-12

- Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet
- Vol. 36, Issue 6, Pages: 371-378(2022)
- 作者机构：
  
  1. 信阳农林学院林学院,河南,信阳,464000
  2. 西藏农牧学院资源与环境学院,西藏,林芝,860000
  3. 周口市农业农村局土壤肥料工作站,河南,周口,466099
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2022.06.045
  CLC： S154.37
- Published：2022
- 稿件说明：
移动端阅览
CAO Lihua, LIU Heman, YANG Hong, et al. Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet[J]. 2022, 36(6): 371-378.
DOI：

CAO Lihua, LIU Heman, YANG Hong, et al. Soil Microbial Distribution and Fungal Community Composition at Different Altitudes on Sejila Mountain, Southeastern Tibet[J]. 2022, 36(6): 371-378. DOI： 10.13870/j.cnki.stbcxb.2022.06.045.

摘要

为探究不同海拔高度土壤微生物的分布特征

以西藏东南部色季拉山为研究区

以不同海拔高度(4 606

4 110

4 000

3 900

3 440 m)土壤为研究对象

分析0—5

5—10 cm 2个层次土壤可培养微生物数量分布特征

并采用高通量测序技术分析了真菌群落组成的丰富度和多样性

探讨了真菌群落组成的主要影响因素。结果表明

供试区土壤可培养微生物数量表现为细菌＞放线菌＞真菌

真菌数量与海拔高度显著相关

而细菌和放线菌数量随海拔高度无明显变化规律;5个海拔高度下共检测到真菌4个门、18个纲、47个目、74个科、93个属;在门水平上优势真菌为担子菌门(Basidiomycota)和子囊菌门(Ascomycota)

纲分类水平上担子菌门下的伞菌纲(Agaricomycetes)相对丰度最高。Alpha多样性分析表明

低海拔区川滇高山栎林和高海拔区高寒草地土壤真菌丰度和多样性均较高

海拔3 900~4 110 m的中部海拔区的雪山杜鹃和急尖长苞冷杉林土壤则较低;冗余分析发现

在门和纲分类水平上

土壤有机碳和全氮含量是真菌群落差异的主要影响因素

海拔高度的影响相对较小。研究结果表明

微生物在不同海拔高度上的分布可能主要取决于土壤性质和植被类型。

Abstract

In order to elucidate the distribution characteristics of soil microorganisms at different altitudes

we selected Sejila mountain as a model area

a typical forest ecological area in southeastern Tibet. In this study we investigated the cultivable microorganisms and the richness of fungal community composition and relative abundances along an altitude gradient (approximately 3 400~4 600 m) in Sejila mountain. The amount of soil cultivable microorganisms in the 0—5 and 5—10 cm layer showed the following rank order: bacteria＞actinomycetes＞fungi

and the number of fungi was significantly correlated with altitude

while the number of bacteria and actinomycetes had no obvious change with altitude. The high-throughput sequencing results showed that there were 4 phyla

18 classes

47 orders

74 families

and 93 genera of fungi detected. Basidiomycota and Ascomycota were predominant fungal at the phylum level

and Agaricomycetes had the highest relative abundance at the classes level. Alpha diversity analysis showed that the soil fungal abundance and diversity of the Quercus aquifolioides forest in lower altitude and the alpine grassland in the higher altitude area were higher

while the Rhododendron aganiphum and Abies georgei var. smithii forest in the middle altitude (3 900~4 110 m) was lower. The redundancy analysis found that

at the phylum and class classification level

the fungal community was mainly caused by soil organic carbon and total nitrogen contents

while the influence of altitude was relatively small. The results suggest that the distribution of microorganisms at different elevations may mainly depend on soil properties and vegetation type.

关键词

Keywords

references

Bardgett R, Freeman C, Ostle N. Microbial contributions to climate change through carbon cycle feedbacks[J].The ISME Journal,2008,2(8):805-814.

Li Z L, Tian D S, Wang B X, et al. Microbes drive global soil nitrogen mineralization and availability[J].Global Change Biology,2019,25(3):1078-1088.

姚宝辉,王缠,张倩,等.甘南高寒草甸退化过程中土壤理化性质和微生物数量动态变化[J].水土保持学报,2019,33(3):138-145.

周际海,郜茹茹,魏倩,等.旱地红壤不同土地利用方式对土壤酶活性及微生物多样性的影响差异[J].水土保持学报,2020,34(1):327-332.

Delgado-Baquerizo M. Obscure soil microbes and where to find them[J].The ISME Journal,2019,13(8):2120-2124.

Ren Z, Wang Z M, Wang Y, et al. Soil bacterial communities vary with grassland degradation in the Qinghai Lake watershed[J].Plant and Soil,2021,460(1):541-557.

李敏,郝伟.大兴安岭4个树种根围土壤细菌群落结构[J].生态学杂志,2021,40(7):2057-2066.

Habtewold J Z, Helgason B L, Yanni S F, et al. Litter composition has stronger influence on the structure of soil fungal than bacterial communities[J].European Journal of Soil Biology,2020,98:e103190.

Malik A A, Chowdhury S, Schlager V, et al. Soil fungal: Bacterial ratios are linked to altered carbon cycling[J].Frontiers in Microbiology,2016,7:e1247.

Romaní A M, Fischer H, Mille-Lindblom C, et al. Interactions of bacteria and fungi on decomposing litter: Differential extracellular enzyme activities[J].Ecology,2006,87:2559-2569.

Siles J A, Margesin R. Seasonal soil microbial responses are limited to changes in functionality at two alpine forest sites differing in altitude and vegetation[J/OL].Scientific Reports,2017,7(1):2204.DOI: 10.1038/s41598-017-02363-2.

周焱,徐宪根,王丰,等.武夷山不同海拔梯度土壤微生物生物量、微生物呼吸及其商值(qMB, qCO

)[J].生态学杂志,2009,28(2):265-269.

李益,冯秀秀,赵发珠,等.秦岭太白山不同海拔锐齿栎林土壤微生物群落的变化特征[J].林业科学,2021,57(12):22-31.

Yang F, Niu K C, Collins C G, et al. Grazing practices affect the soil microbial community composition in a Tibetan alpine meadow[J].Land Degradation and Development,2019,30(1):49-59.

Dong L, Li J J, Sun J, et al. Soil degradation influences soil bacterial and fungal community diversity in overgrazed alpine meadows of the Qinghai-Tibet Plateau[J].Scientific Reports,2021,11(1):e11538.

东主,李瑛萍,石玉龙,等.色季拉山不同海拔土壤可溶性有机碳与养分分布特征及影响因素研究[J].高原农业,2019,3(6):587-596,605.

鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.

吴金水.土壤微生物生物量测定方法及其应用[M].北京:气象出版社,2006.

Schloss P D, Westcott S L, Ryabin T, et al. Introducing mothur: Open source, platform independent, community-supported software for describing and comparing microbial communities[J].Applied and Environmental Microbiology,2009,75(23):7537-7541.

刘旻霞,蒋晓轩,李全弟,等.兰州北山不同植被土壤可培养微生物丰度变化特征及影响因素[J].中国环境科学,2020,40(6):2683-2691.

马进鹏,庞丹波,陈林,等.贺兰山东坡不同海拔典型植被带土壤微生物磷酸脂肪酸分析[J].生态学报,2022,42(12):5045-5058.

韦玮,丁贵杰,陈伟,等.一、二代马尾松林土壤微生物数量及酶活性垂直分布特征[J].重庆师范大学学报(自然科学版),2017,34(3):114-120.

Güsewell S, Gessner M O. N∶P ratios influence litter decomposition and colonization by fungi and bacteria in microcosms[J].Functional Ecology,2009,23(1):211-219.

曹丽花,尹为玲,刘合满,等.西藏东南部色季拉山主要类型森林叶片和枯落物养分含量特征[J].生态学报,2019,39(11):4029-4038.

杨红,柳文杰,刘合满,等.高寒森林植物叶片—枯落物—土壤养分含量及化学计量特征[J].浙江大学学报(农业与生命科学版),2021,47(5):607-618.

张地,张育新,曲来叶,等.海拔对辽东栎林地土壤微生物群落的影响[J].应用生态学报,2012,23(8):2041-2048.

Xu M, Li X L, Cai X B, et al. Soil microbial community structure and activity along a montane elevational gradient on the Tibetan Plateau[J].European Journal of Soil Biology,2014,64:6-14.

Hanson C A, Allison S D, Bradford M A, et al. Fungal taxa target different carbon sources in forest soil[J].Ecosystems,2008,11(7):1157-1167.

周晨霓,马和平.西藏色季拉山典型植被类型土壤活性有机碳分布特征[J].土壤学报,2013,50(6):1246-1251.

姜雪薇,马大龙,臧淑英,等.高通量测序分析大兴安岭典型森林土壤细菌和真菌群落特征[J].微生物学报,2021,48(4):1093-1105.

满百膺,向兴,罗洋,等.黄山典型植被类型土壤真菌群落特征及其影响因素[J].菌物学报,2021,40(10):2735-2751.

Shi L L, Mortimer P E, Slik J W F, et al. Variation in forest soil fungal diversity along a latitudinal gradient[J].Fungal Diversity,2014,64(1):305-315.

Newsham K K, Hopkins D W, Carvalhais L C C, et al. Relationship between soil fungal diversity and temperature in the maritime Antarctic[J].Nature Climate Change,2016,6(2):182-186.

向前胜,张登山,孙奎,等.高寒地区不同海拔梯度西北小檗生境土壤微生物群落结构及多样性分析[J].西北植物学报,2021,41(6):1036-1050.

Liu J, Liu M, Wu M, et al. Soil pH rather than nutrients drive changes in microbial community following long-term fertilization in acidic Ultisols of southern China[J].Journal of Soils and Sediments,2018,18:1853-1864.

Zhalnina K, Dias R, de Quadros P D, et al. Soil pH determines microbial diversity and composition in the park grass experiment[J].Microbial Ecology,2015,69(2):395-406.

Yang Y F, Gao Y, Wang S P, et al. The microbial gene diversity along an elevation gradient of the Tibetan grassland[J].The ISME Journal,2014,8(2):430-440.

Zeng Q C, Liu Y, Xiao L, et al. Climate and soil properties regulate soil fungal communities on the loess plateau[J].Pedobiologia,2020,81/82:e150668.

Views

753

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Research Progress on the Effects of Hydraulic Erosion on Soil Carbon Cycle and Soil Microbial Diversity

Soil Microorganism and Enzyme Activities Under Different Oasis Farmlands in the Ulanbuh Sandy Areas

Effect of Application on Different Amount of Red Mud on Rice Yield and Soil Biological Properties in Cd-contaminated Paddy Soil

Effects of Reduced Chemical Fertilizer with Application of Bio-organic Fertilizer on Rape Growth, Microorganism and Enzymes Activities in Soil

Dynamic Characteristics of Soil Microorganisms, Enzymes and Available Nutrients Under the Conditions of the Corn Straw Returning Based on Rotary Tillage

Related Author

SUN Liying

CAI Qiangguo

LIU Na

YANG Wenbo

SUN Fei

XIN Zhi-ming

XU Ming-gang

徐明岗

Related Institution

College of Resources and Environment, University of Chinese Academy of Sciences

Key Laboratory of Water Cycle and Related Land Surface Processes, CAS, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences

College of Bioengineering, Henan University of Technology

中国林业科学研究院沙漠林业实验中心

BAO Yao-xian1,2,XU Ming-gang1,XIN Zhi-ming2,SUN Fei2,XU Jun2(1.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,2.Experimental Center of Desert Forestry,Chinese Academy of Forestry,Dengkou,)

⁰