Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area

CHANG Daoqin; SONG Naiping; YUE Jianmin; MEN Chen; MA Yun

doi:10.13870/j.cnki.stbcxb.2022.04.045

您当前的位置：

首页 >

文章列表页 >

Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area

更新时间：2025-03-12

- Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area
- Vol. 36, Issue 4, Pages: 365-374(2022)
- 作者机构：
  
  1. 宁夏大学生态环境学院,银川,750021
  2. 宁夏大学西北土地退化与生态系统恢复国家重点实验室培训基地,银川,750021
  3. 西北退化生态系统恢复与重建教育部重点实验室,银川,750021
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2022.04.045
  CLC： X53
- Published：2022
- 稿件说明：
移动端阅览
CHANG Daoqin, SONG Naiping, YUE Jianmin, et al. Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area[J]. 2022, 36(4): 365-374.
DOI：

CHANG Daoqin, SONG Naiping, YUE Jianmin, et al. Effect of Microbial Induced Calcium Carbonate Precipitation on the Pollution Control of Copper Tailings in Arid and Semi-Arid Area[J]. 2022, 36(4): 365-374. DOI： 10.13870/j.cnki.stbcxb.2022.04.045.

摘要

为明确微生物诱导碳酸钙沉淀技术（microbial induced calcium carbonate precipitation，MICP）在干旱半干旱高风蚀地区重金属尾矿治理效果，以研究区生境土中筛选的碳酸盐矿化细菌—纺锤型赖氨酸芽孢杆菌（Lysinibacillus fusiformis）为研究对象，通过在尾矿渣中加注纺锤型赖氨酸芽孢杆菌菌液和由1 mol/L的尿素及氯化钙配制成的胶结液进行室内模拟试验，研究MICP处理前后尾矿坡顶、坡中、坡底（坡顶—细颗粒（K1<5 mm）、坡中—中颗粒（5 mm15 mm））不同粒径尾矿渣中重金属元素含量、pH、电导率及酶活性等基础理化性质的变化，利用双因素方差分析和相关性热图比较分析揭示基于生境微生物的MICP修复技术对区域不同粒径尾矿渣污染物治理的效果及确定MICP技术对不同粒径尾矿渣修复的菌剂用量。结果表明，采用MICP技术对尾矿渣中重金属含量、pH、电导率及酶活性等基础理化性质有显著影响。MICP处理下细颗粒（K1<5 mm）尾矿渣中As降低21%，中颗粒（5 mm15 mm）中Pb降低22%。K1、K3中的pH降低5%，K2降低4%。K1中全钾、脲酶均升高33%（p<0.05），K2中全碳升高39%（p<0.05），K3中蔗糖酶升高7%（p<0.05），微生物碳氮在K1、K2、K3中显著升高（p<0.05）。同时MICP处理下Ni与Pb、As与Cu、电导率与Pb等呈极显著正相关（p<0.01），pH与Pb呈极显著负相关（p<0.01），在MICP技术处理后，全碳、全氮、全钾、全磷及有机质和重金属元素含量间的相关性增强。综上所述基于生境微生物的MICP修复技术能够降低尾矿渣重金属元素含量，调节尾矿渣土体养分状况从而调节污染土体质量，降低由矿产开发导致的环境污染风险。同时，进一步确定不同颗粒尾矿渣达到修复治理水平所需菌剂用量，具有污染土质治理修复应用价值。

Abstract

In order to clarify the treatment effect of Microbial Induced Calcium Carbonate Precipitation (MICP) in the heavy metal tailings in highly wind erosion areas in arid and semi-arid region

this paper used Bacillus (Lysinibacillus fusiformis) as the research object

which was the carbonate-mineralizing bacteria-spindle lysine bacillus screened in the habitat soil of the study area. We added the bacillus fermented liquid and the cementing solution mixed by 1mol/L urea and calcium chloride in the tailings slag to carry out indoor simulation experiments. And we compared the varieties of basic physical and chemical properties (heavy metal element content

electrical conductivity and enzyme activity) in tailing slag with different size which were from slope top (fine particles:K1<5 mm)

slope middle (middle particles:5 mm15 mm) before and after MICP treatment. And according to the method of two factor analysis of variance and correlation comparative analysis

we revealed the effect of MICP remediation technology based on habitat microorganisms on the regulation of regional tailing slag pollutants and determined the dosage of bacterial agent in MICP technology for the remediation of tailing slag with different particle sizes. The results showed that the MICP treatment had a significant effect on the basic physical and chemical properties such as heavy metal content

electrical conductivity and enzyme activity in tailings slag. Under the MICP treatment

the arsenic (As) content of small particles (K1<5 mm) reduced by 21%

the mercury (Hg) of medium particles (5 mm15 mm) decreased by 22%. The pH in K1 and K3 reduced by 5% and K2 reduced by 4%. The total potassium (K) and urease in K1 significantly increased by 33% (p<0.05)

the total carbon in K2 increased obviously by 39% (p<0.05)

and the sucrase in K3 increased by 7% (p<0.05). The amount of microbial carbon and nitrogen significantly increased in K1

K2 and K3 (p<0.05). At the same time

Ni-Pb

As-Cu

electrical conductivity and Pb were significantly positively correlated (p<0.01) under MICPtreatment and pH-Pb was extremely significantly negatively correlated (p<0.01). And after MICP treatment

the correlations among total nitrogen

total potassium

total phosphorus

the contents of organic matter

heavy metal elements were enhanced.In summary

the MICP remediation technology based on habitat microorganisms could reduce the content of heavy metal elements amount in tailings slag

adjust the nutrient status of tailings slag soil

so as to regulate the quality of contaminated soil

and reduce the risk of environmental pollution caused by mineral resources development. At the same time

this study further determined the application dosage of bacterial agent for different particle tailings restoration to reach the remediation level

which has the better application value in the treatment and remediation of polluted soil.

关键词

Keywords

references

曾蓉.土壤重金属污染现状及修复研究[J].安徽农学通报, 2012, 18(20):20, 32.

全国土壤污染状况调查公报[J].中国环保产业, 2014(5):10-11.

郝秀珍, 周东美.金属尾矿砂的改良和植被重建研究进展[J].土壤, 2005, 37(1):13-19.

陈卫连, 姚锁平, 蔡婕, 等.一种铅尾矿砂的植被改良方法:CN 102658291A[P].2014-05-07.

周科平, 林允, 胡建华, 等.大脚岭铅锌尾矿库重金属迁移规律与污染评价[J].中南大学学报(自然科学版), 2015, 46(5):1953-1958.

赵仁鑫, 郭伟, 包玉英, 等.内蒙古草原白乃庙铜矿区土壤重金属污染特征研究[J].土壤通报, 2012, 43(2):496-500.

叶增辉, 尹国勋.污染土壤的物理化学修复现状与展望[J].山西建筑, 2010, 36(13):333-335.

李榜江.贵州山区煤矿废弃地重金属污染评价及优势植物修复效应研究[D].重庆:西南大学, 2014.

赵庆龄, 张乃弟, 路文如.土壤重金属污染研究回顾与展望Ⅱ:基于三大学科的研究热点与前沿分析[J].环境科学与技术, 2010, 33(7):102-106.

Shraddha G, Darshan M. Microbiallyinduced calcite precipitation through urolytic organisms:A review[J].International Journal of Life Sciences, 2019, 7(1):133-139.

Seifan M, Samani A K, Berenjian A. Bioconcrete:Next generation of self-healing concrete[J].Applied Microbiology and Biotechnology, 2016, 100(6):2591-2602.

Helmi F M, Elmitwalli H R, Elnagdy S M. Calcium carbonate precipitation induced by ureolytic bacteria Bacillus licheniformis[J].Ecological Engineering, 2016, 90:367-371.

Kang C H, Shin Y J, Anbu P, et al. Biosequestration of copper by bacteria isolated from an abandoned mine by using microbially induced calcite precipitation[J].The Journal of General and Applied Microbiology, 2016, 62(4):206-212.

王新花, 赵晨曦, 潘响亮.基于微生物诱导碳酸钙沉淀(MICP)的铅污染生物修复[J].地球与环境, 2015, 43(1):80-85.

Zhao Y, Yao J, Yuan Z M, et al. Bioremediation of Cd by strain GZ-22 isolated from mine soil based on biosorption and microbially induced carbonate precipitation[J].Environmental Science and Pollution Research, 2020, 24(1):372-380.

Li M, Cheng X H, Guo H X. Heavy metal removal by biomineralization of urease producing bacteria isolated from soil[J].International Biodeterioration and Biodegradation, 2013, 76:81-85.

陈海杰, 马娜, 白金峰, 等.基于外供氢气-氢化物-原子荧光光谱法测定地球化学样品中硒的研究[J].光谱学与光谱分析, 2020, 40(9):2896-2900.

中华人民共和国国土资源部.DZ/T 0279.13-2016区域地球化学样品分析方法第13部分:砷、锑和铋量测定氢化物发生-原子荧光光谱法[S].北京:中国标准出版社, 2016.

中华人民共和国国土资源部.DZ/T 0279.17-2016区域地球化学样品分析方法第17部分:汞量测定蒸气发生-冷原子荧光光谱法[S].北京:中国标准出版社, 2016.

李强, 赵秀兰, 胡彩荣.ISO 10390:2005土壤质量pH的测定[J].污染防治技术, 2006, 19(1):53-55.

黄博珠.土壤电导率测定的不确定度评定[J].广州化工, 2018, 46(15):132-133.

鲍士旦.土壤农化分析[M].北京:中国农业出版社, 2000:521-648.

Achal V, Pan X L, Zhang D Y, et al. Bioremediation of Pb-contaminated soil based on microbially induced calcite precipitation[J].Journal of Microbiology and Biotechnology, 2012, 22(2):244-247.

郭同同.耐汞微生物的分离及其对汞污染土壤的修复[D].昆明:云南大学, 2018.

Vrancut L, Badulescu B, Musta S. The influence of pH on the mobility of heavy metals in agricultural soil from Ialomita area[C].International Mutidisciplinary Scientiific Geoconference:SGEM, 2019.

陈敏洁, 李亚飞, 郑春丽.微生物诱导碳酸钙沉淀对土壤中Pb污染稳定化的效果研究[J].有色金属工程, 2020, 10(12):132-138.

沈萍.微生物学[M].北京:高等教育出版社, 2000:1421-1531.

范娜, 彭之东, 赵建武, 等.微生物菌剂对土壤酶活性及高粱生长的影响[J]. 中国农业科技导报, 2021, 23(2):185-192.

胡廷会.干旱胁迫下LCO和TH17对燕麦形态、生理指标及根际土壤环境的影响[D].呼和浩特:内蒙古农业大学, 2014.

李楠.重金属形态与土壤酶活性关系研究[D].西安:西安科技大学, 2015.

陈玲玲.土壤酶活性对土壤重金属污染的指示研究[D].西安:西安科技大学, 2012.

Sannino F, Gianfreda L. Pesticide influence on soil enzymatic activities[J].Chemosphere, 2001, 45(4/5):417-425.

陈亚东, 梁成华, 张永红, 等.氧化还原条件对湿地土壤磷吸附与解吸特性的影响[J].生态学杂志, 2010, 29(4):116-121.

叶协锋, 杨超, 敬海霞, 等.绿肥对植烟土壤酶活性及土壤肥力的影响[J].植物营养与肥料学报, 2013, 19(2):445-454.

张玲, 叶正钱, 李廷强, 等.铅锌矿区污染土壤微生物活性研究[J].水土保持学报, 2006, 20(3):136-140.

杨强, 刘明亮, 沈旭.钱塘江沉积物重金属污染源解析及生态风险评价[J].环境科学与技术, 2017, 40(12):278-283.

陈岭啸, 宋垠光, 袁旭音, 等.长江三角洲典型地区土壤-水稻系统中Cd的分布及其迁移制约因素[J].地球科学与环境学报, 2011, 33(3):288-295.

孙晨, 程一松, 王兴明, 等.植被恢复对露天铁矿排土场土壤理化性质及重金属污染特征的影响[J].现代矿业, 2017, 33(4):218-221.

Views

687

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Solidification Effect of Calcium Carbonate Precipitation Induced by Plant and Microorganism on Copper Tailings

Effects of Long-term Straw Returning on Heavy Metals of Soil and Agricultural Products in the Polluted Farmland

Related Author

CHANG Daoqin

SONG Naiping

YUE Jianmin

MENG Chen

ZHENG Shun'an

LIU Daili

ZHANG Mingkui

WANG Jingwen

Related Institution

Key Laboratory of Land Degradation and Ecosystem Restoration in Northwest China, Ningxia University

Key Laboratory of Rehabilitation and Reconstruction of Degraded Ecosystems in Northwest China

Agricultural and Rural Ecology and Resource Protection Station

Key Laboratory of Resource Recycling Technology and Model, Ministry of Agriculture and Rural Areas

College of Environmental and Resource Sciences,Zhejiang University

⁰