Effects of Litter and Root Inputs Changing on Soil Microbial Community in a Natural

ZHANG Lei; JIA Shuxian; LI Xiaoling; LU Yuming; LIN Weisheng; LIU Xiaofei; GUO Jianfen

doi:10.13870/j.cnki.stbcxb.2021.06.037

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Effects of Litter and Root Inputs Changing on Soil Microbial Community in a Natural

更新时间：2025-03-12

- Effects of Litter and Root Inputs Changing on Soil Microbial Community in a Natural
- Vol. 35, Issue 6, Pages: 270-277(2021)
- 作者机构：
  
  1. 福建师范大学地理科学学院,福州,350007
  2. 湿润亚热带生态-地理过程教育部重点实验室,福州,350007
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2021.06.037
  CLC： S154.3
- Published：2021
- 稿件说明：
移动端阅览
ZHANG Lei, JIA Shuxian, LI Xiaoling, et al. Effects of Litter and Root Inputs Changing on Soil Microbial Community in a Natural[J]. 2021, 35(6): 270-277.
DOI：

ZHANG Lei, JIA Shuxian, LI Xiaoling, et al. Effects of Litter and Root Inputs Changing on Soil Microbial Community in a Natural[J]. 2021, 35(6): 270-277. DOI： 10.13870/j.cnki.stbcxb.2021.06.037.

摘要

伴随气候变化下亚热带地区米槠天然林净初级生产力变化，凋落物以及植物根系输入亦会发生改变，这将显著影响土壤微生物群落。于2019年7月在设置7年的米槠天然林植物残体添加和去除试验（the detritus input and removal treatments，DIRT）样地采集不同处理（对照、去除地上凋落物、去除地下根系、无凋落物输入、添加双倍地上凋落物）的2个土层土壤（0—10，10—20 cm），测定微生物磷脂脂肪酸（phospholipid fatty acid，PLFA）含量，计算各微生物群落比值以及多样性，进一步揭示凋落物和植物根系输入对亚热带米槠天然林土壤微生物群落组成和多样性的影响。结果表明：（1）不同处理下0—10 cm土层微生物磷脂脂肪酸含量约为10—20 cm土层的2倍；（2）地上凋落物变化均使得革兰氏阳性菌、阴性菌及放线菌等细菌含量出现不同程度的下降，但不会对丛枝菌根等真菌含量产生影响，而去除根系处理显著降低丛枝菌根真菌含量；（3）微生物群落Shannon-wiener、Simpson多样性指数不受凋落物输入的影响，凋落物去除降低表层土壤微生物群落的Margalef丰富度，提高Pielou均匀度，表明0—10 cm土层微生物群落含量与分布状况受凋落物输入变化影响较大；（4）地下植物根系存在可提高真菌（如丛枝菌根真菌）含量，而地上凋落物输入主要改变细菌丰度以及群落结构。可溶性有机碳以及矿质氮是影响不同处理土壤微生物群落组成和多样性的主要因素。可见，凋落物和根系输入通过土壤理化性质的变化而影响了微生物群落，研究结果可为全面认识植物、土壤与微生物间的相互作用对森林生产力的影响提供科学依据。

Abstract

With the change of net primary productivity in a natural forest of Castanopsis carlesii in subtropical region under climate change

litter and plant root inputs will also change

which will significantly affect soil microbial community. In this study

the Detritus Input and Removal Treatments (DIRT) had been set up in a natural C. carlesii forest for seven years. In July 2019

soils of two layers (0—10

10—20 cm) indifferent treatments (control

removal of above-ground litters

removal of roots

no input of litters

and addition of double above-ground litters) were collected to measure microbial phospholipid fatty acid (PLFA) contents. The ratio and diversity of microbial groups were calculated to further reveal the influencing mechanism of litter and plant root inputs on soil microbial community composition and diversity in a subtropical natural C. carlesii forest. The results showed that: (1) The PLFA content of microorganisms in the 0—10 cm soil layer for different treatments was about twice that in the 10—20 cm soil layer. (2) The addition or removal of litters reduced the contents of gram-positive bacteria

gram-negative bacteria

actinomycetes and other bacteria to varying degrees

but had no effect on arbuscular mycorrhizal fungi. The root removal reduced the PLFA content of arbuscular mycorrhizal fungi significantly. (3) Shannon-Wiener and Simpson diversity indices of the microbial communities were not affected by different treatments. Litter removal reduced the richness of soil microbial community and improved the evenness. The content and distribution of microbial community in 0—10 cm soil layer were greatly affected by the change of litter input. (4) Plant roots improved the content of fungi

while litter input mainly changed bacterial abundance and structure. Soluble organic carbon and mineral nitrogen were the main factors that affect the microbial community composition and diversity. There fore

litter and root inputs affected soil microbial community through the change of soil properties. The results can provide a scientific basis for understanding the effects of interactions among plants

soil

and microorganisms on forest productivity.

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