Effects of Double Addition or Removal of Litter on Soil Hydrolases Activities and Their Stoichiometry in

LU Yuming; XU Enlan; WU Dongmei; LU Shengxu; LIU Xiaofei; GUO Jianfen

doi:10.13870/j.cnki.stbcxb.2021.04.043

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Effects of Double Addition or Removal of Litter on Soil Hydrolases Activities and Their Stoichiometry in

更新时间：2025-03-12

- Effects of Double Addition or Removal of Litter on Soil Hydrolases Activities and Their Stoichiometry in
- Vol. 35, Issue 4, Pages: 313-320(2021)
- 作者机构：
  
  1. 福建师范大学地理科学学院,福州,350007
  2. 福建师范大学湿润亚热带山地生态国家重点实验室培育基地,福州,350007
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2021.04.043
  CLC： S154.1
- Published：2021
- 稿件说明：
移动端阅览
LU Yuming, XU Enlan, WU Dongmei, et al. Effects of Double Addition or Removal of Litter on Soil Hydrolases Activities and Their Stoichiometry in[J]. 2021, 35(4): 313-320.
DOI：

LU Yuming, XU Enlan, WU Dongmei, et al. Effects of Double Addition or Removal of Litter on Soil Hydrolases Activities and Their Stoichiometry in[J]. 2021, 35(4): 313-320. DOI： 10.13870/j.cnki.stbcxb.2021.04.043.

摘要

土壤酶与土壤养分紧密相关，并能够活化土壤中营养元素从而改善土壤环境质量，为更加深入了解全球气候变化背景下森林凋落物数量的变化及其酶化学计量比的影响，以亚热带米槠林为研究对象设计随机区组试验，设置3种凋落物处理（移除凋落物（NRNL）、添加双倍凋落物（NRDL）、保留凋落物（CK）），通过测定土壤微生物生物量碳、氮和水解酶活性（β—葡萄糖苷酶（βG）、纤维素水解酶（CBH）、β—N—乙酰氨基葡萄糖苷酶（NAG）、亮氨酸氨基肽酶（LAP）、酸性磷酸酶（AP）），探讨排除根系作用下双倍添加和移除地上凋落物对米槠林土壤养分状况和土壤生态酶化学计量特征的影响。结果表明，与对照（CK）相比，NRDL处理显著提高了CBH和LAP活性，同时提高了土壤总碳（TC）、全氮（TN）、矿质氮、可溶性有机碳（DOC）、微生物生物量氮（MBN）含量，而NRNL处理只降低了土壤MBC含量。同时发现，土壤生态酶化学计量比未受到凋落物输入处理的影响，CK、NRNL和NRDL处理土壤酶活性比ln（βG+CBH）∶ln（NAG+LAP）分别为1.25，1.36，1.30；ln（βG+CBH）∶ln（AP）分别为0.78，0.78，0.83；ln（NAG+LAP）∶ln（AP）分别为0.62，0.58，0.64。相关分析表明，TC、TN、矿质氮、DOC、DON、MBC和MBN分别与βG、CBH、AP和LAP活性呈显著正相关；冗余分析表明，DOC是驱动土壤水解酶活性和化学计量比变化的关键因子。综上，NRDL通过增加土壤DOC的浓度，从而提高土壤水解酶活性，研究结果可为亚热带森林土壤养分管理提供基础数据。

Abstract

Soil enzymes are closely linked with nutrients and chemical properties of soil. Soil enzymes can activate nutrient elements in soil to improve soil quality. Changes in net primary productivity in response to climate change are likely to affect litter inputs to forest soil. However

the effects of altered litter input on soil enzymes activities and their stoichiometry remain poorly understood in Castanopsis carlesii forest. In this study

soil hydrolases β—glucosidase enzyme (βG)

cellulose (CBH)

β—N—acetylglucosaminidase (NAG)

leucine aminopeptidase (LAP)

and acid phosphatase (AP) activities and their stoichiometric characteristics under different litter input treatments with root exclusion

viz. no litter (NRNL)

double litter (NRDL)

control (CK) were measured in a subtropical Castanopsis carlesii forest in Sanming

Fujian

China. The results showed that compared with CK

NRDL significantly improved CBH and LAP activities

and increased the contents of soil total carbon (TC)

total nitrogen (TN)

mineral nitrogen

dissolved organic carbon (DOC)

and microbial biomass nitrogen (MBN)

while the content of MBC was only reduced under NRNL. It was also found that the soil enzyme stoichiometric ratio was not affected by litter input treatments. Moreover

the values of ln (βG+CBH)∶ln (NAG+LAP) were 1.25

1.36

1.30; the values of ln (βG+CBH) ∶ln (AP) were 0.78

0.78

0.83; the values of ln (βG+CBH) ∶ln (NAG+LAP) were 1.25

1.36

1.30; ln (NAG+LAP)∶ln (AP) were 0.62

0.58

0.64. The correlation analysis showed that TC

mineral nitrogen

DOC

DON

MBC and MBN were significantly positively correlated with βG

CBH

AP and LAP activities. Redundancy analysis indicated that the changes in hydrolases activities and their stoichiometry were mainly driven by soil DOC concentration. Thus

NRDL could improve the hydrolase activity by increasing the concentration of soil DOC. This study could provide a basic theory for soil nutrient management in subtropical forests.

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