Promoting Effect of Exogenous Additives on Propagation of Moss Biocrusts in Sand-land

BAI Xueqiang; TIAN Chang; LI Yahong; JU Mengchen; WANG Chun; WEI Yingxin; BU Chongfeng

doi:10.13870/j.cnki.stbcxb.2020.06.025

您当前的位置：

首页 >

文章列表页 >

Promoting Effect of Exogenous Additives on Propagation of Moss Biocrusts in Sand-land

更新时间：2025-03-12

- Promoting Effect of Exogenous Additives on Propagation of Moss Biocrusts in Sand-land
- Vol. 34, Issue 6, Pages: 172-177(2020)
- 作者机构：
  
  1. 西北农林科技大学水土保持研究所, 陕西杨凌,712100
  2. 中国科学院水利部水土保持研究所, 陕西杨凌,712100
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2020.06.025
  CLC： S154.4;S157.9
- Published：2020
- 稿件说明：
移动端阅览
BAI Xueqiang, TIAN Chang, LI Yahong, et al. Promoting Effect of Exogenous Additives on Propagation of Moss Biocrusts in Sand-land[J]. 2020, 34(6): 172-177.
DOI：

BAI Xueqiang, TIAN Chang, LI Yahong, et al. Promoting Effect of Exogenous Additives on Propagation of Moss Biocrusts in Sand-land[J]. 2020, 34(6): 172-177. DOI： 10.13870/j.cnki.stbcxb.2020.06.025.

摘要

人工苔藓结皮作为一种新型的防风固沙潜在途径而备受关注，为进一步优化现有的培育扩繁技术，采用4因素2水平正交试验设计，探讨了菌类（放线菌和巨大芽孢杆菌）、藻类（小球藻和具鞘微鞘藻）、沙蒿胶（有和无）和基质类型（沙土基质和配方基质）对沙地苔藓结皮室内发育扩繁的影响。结果表明：（1）4因素中仅基质类型显著影响了苔藓结皮的盖度和株密度（P<0.05），沙土基质处理的苔藓结皮盖度和株密度均值比配方基质处理分别提高61.04%和17.48株/cm2；（2）藻类、菌类和基质类型显著影响了苔藓的生理特性（P<0.05），而沙蒿胶影响不显著。沙土基质、放线菌和小球藻的添加显著提高了苔藓的过氧化物酶（POD）活性和可溶性蛋白含量，降低了丙二醛（MDA）含量；（3）最适扩繁组合为"沙土基质+放线菌（1 g/kg基质）+小球藻（2 g/m2）"，在30天内，上述组合在培养箱条件下（光照设定为6 000 lx，光周期12 h/d，温度20℃，空气湿度75%），可将1 m2野外苔藓种源培育为4 m2盖度达97.14%的苔藓结皮，实现4倍扩繁。研究结果将为今后大规模工厂化高效扩繁苔藓种源提供技术支撑。

Abstract

Artificial moss biocrusts (moss-dominating biological soil crusts) have attracted attention as a new type of windproof and sand fixation method in recent years. In order to further optimize the existing breeding and propagation technology

this study used a 4 factor and 2 level orthogonal test design to discuss the effects of microorganism (Actinomyces bovis

Bacillus megaterium)

algae (Chlorella

Microcolusvaginatus)

artemisia gum (yes

no)

and substrate type (sand soil matrix

formulation matrix) on the indoor propagation of sand-land moss biocrusts. The results showed that: (1) Among the four factors

only the substrate type significantly affected the coverage and plant density of moss biocrusts (P<0.01)

which increased to 61.04% and 17.48 strains/cm2

were higher than that of the formulation substrate treatment. (2) Algae

microorganism

and substrate types significantly influenced the physiological characteristics of moss biocrusts (P<0.01)

while the effect of artemisiagum was not significant. The sandy substrates and the addition of actinomycetes and chlorella significantly increased the peroxidase (POD) activity and soluble protein content and reduced the content of malondialdehyde (MDA) of moss biocrusts. (3)The optimal treatment for propagation was sandy substrate + actinomycete (1 g/kg substrate) + chlorella (2 g/m2). Under the conditions of the incubator (set illumination

photoperiod

temperature

air humidity as 6 000 lx

12 h/d

20 ℃

75%)

we propagated 1 m2 moss biocrusts (collected from the field and fragmented into provenance) to 4 m2 moss biocrusts with a coverage of 97.14%

which achieved 4 times multiplication. This research would provide technical support for large-scale industrialization and efficient propagation of moss biocrusts in the future.

关键词

Keywords

references

Elbert W, Weber B, Büdel B, et al. Microbiotic crusts on soil, rock and plants:Neglected major players in the global cycles of carbon and nitrogen?[J].Biogeoscience Discussion,2009,6:6983-7015.

魏江春.沙漠生物地毯工程:干旱沙漠治理的新途径[J].干旱区研究,2005,22(3):287-288.

Lan S B, Wu L, Zhang D L, et al. Successional stages of biological soil crusts and their microstructure variability in Shapotou region (China)[J].Environmental Earth Science,2012,65:77-88.

李金峰,孟杰,叶菁,等.陕北水蚀风蚀交错区生物结皮的形成过程与发育特征[J].自然资源学报,2014,29(1):67-78.

徐杰,白学良,田桂泉,等.干旱半干旱地区生物结皮层藓类植物氨基酸和营养物质组成特征及适应性分析[J].生态学报,2005,25(6):1247-1255.

牛玉璐,曹永胜.生物结皮及其在荒漠治理中的作用[J].生物学教学,2005,30(10):5-6.

Grettarsdottir J, Aradottir A L, Vandvik V, et al. Long term effects of reclamation treatments on plant succession in Iceland[J].Restoration Ecology,2004,12:268-278.

张元明,王雪芹.荒漠地表生物土壤结皮形成与演替特征概述[J].生态学报,2010,30(16):4484-4492.

陈彦芹.黄土丘陵区苔藓结皮人工培养方法试验研究[D].陕西杨凌:西北农林科技大学,2009.

卜崇峰,杨建振,张兴昌.毛乌素沙地生物结皮层藓类植物培育试验研究[J].中国沙漠,2011,31(4):937-941.

卜崇峰,张侃侃,杨永胜.一种沙地苔藓结皮的快速培育方法:中国, CN104350932A[P].2015-02-18.

刘敦华,谷文英,丁霄霖.沙蒿胶对面团流变性质的影响及在面包加工中的应用[J].农业工程学报,2009,25(增刊1):233-236.

陈兰周,刘永定,李敦海,等.荒漠藻类及其结皮的研究[J].中国科学基金,2003,17(2):90-93.

蔡燕飞,廖宗文.土壤微生物生态学研究方法进展[J].土壤与环境,2002,11(2):167-171.

莫宏伟,任志远.近30年陕北榆阳区植被土壤保持效益动态[J].生态学杂志,2009,28(4):626-631.

Xu S J, Yin C S, He M, et al. A technology for rapid reconstruction of moss-dominated soil crusts[J].Environmental Engineering Science,2008,25(8):1129-1137.

徐丽萍,杨改河,姜艳,等.黄土高原人工植被小气候生态效应研究[J].水土保持学报,2008,22(16):163-173.

Li X R, He M Z, Stefan Z, et al. Microgeomorphology determines community structure of BSCs at small scale[J].Earth Surface Process and Landform,2010,35:932-940.

夏钦,何丙辉,刘玉民.高温胁迫对粉带扦插苗形态和生理特征的影响[J].生态学报,2010,30(19):5217-5224.

孙守琴,王根绪,罗辑,等.苔藓植物对环境变化的响应和适应性[J].西北植物学报,2009,29(11):2360-2365.

吴虹玥,包维楷,王安.苔藓植物的化学元素含量及其特点[J].生态学杂志,2005,24(1):58-64.

玄雪梅,王艳,曹同,等.上海地区藓类环境生理学特性的初步研究[J].应用生态学报,2004,15(11):2117-2121.

王显蓉,赵允格,王媛.干旱半干旱地区藓结皮人工培养研究进展[J].西北林学院学报,2014,29(6):66-71.

谢作明,刘永定,陈兰洲,等.不同培养条件对具鞘微鞘藻生物量和多糖产量的影响[J].水生生物学报,2008,32(2):272-275.

Chen J, Li J, Sun A D, et al. Supercritical CO

extraction and pre-column derivatization of polysaccharides from Artemisia sphaerocephala Krasch. Seeds via gas chromatography[J].Industrial Crops and Products,2014,60:138-143.

Yang X J, Baskin C C, Baskin J M, et al. Degradation of seed mucilage by soil microflora promates early seedling growth of a desert sand dune plant[J].Plant Cell and Environment,2012,35(5):872-883.

刘军,张宇清,冯薇,等.几种外源添加物对生物土壤结皮培育的影响[J].北京林业大学学报,2016,38(5):100-107.

李青梅,陆秀君,马里,等.胶质芽孢杆菌菌剂对四种蔬菜种子发芽及幼苗生长的影响[J].北方园艺,2017(1):10-13.

吕爱英,王永歧,沈阿林,等.6种微生物肥料在不同作物上的应用效果[J].河南农业科学,2004,33(4):49-51.

陈念,高向阳,林壁润.万隆霉菌抑制细菌的作用机理初探[J].西北农林科技大学(自然科学版),2005,33(51):143-148.

赵艳,张晓波,郭伟.不同土壤胶质芽孢杆菌生理生化特征及其解钾活性[J].生态环境学报,2009,18(6):2283-2286.

饶本强,刘永定,胡春香,等.人工藻结皮技术及其在沙漠治理中的应用[J].水生生物学报,2009,33(4):756-761.

Doherty K D, Antoninka A J, Bowker M A, et al. A novel approach to cultivate biocrusts for restoration and experimentation[J].Ecological Restoration,2015,33(1):13-16.

王伟波.接种蓝藻修复生物土壤结皮的研究[D].武汉:中国科学院水生生物研究所,2009.

毕永红.念珠藻的接种对半荒漠土壤结构稳定性的作用[C].中国地理学会自然地理专业委员会."土地变化科学与生态建设"学术研讨会论文集.中国地理学会自然地理专业委员会:中国地理学会,2004:569-575.

张丙昌,王敬竹,玛依努尔,等.生物结皮中几种优势藻和齿肋赤藓(

Syntrichia caninervis

Mitt.)种间关系研究[C].生态文明建设中的植物学:现在与未来——中国植物学会第十五届会员代表大会暨八十周年学术年会论文集:植物生态与环境保护,2013.

Views

1320

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

⁰