Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution

LIU Yuechang; LI Baozhen; WANG Tao; WANG Lan

doi:10.13870/j.cnki.stbcxb.2020.04.052

您当前的位置：

首页 >

文章列表页 >

Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution

更新时间：2025-03-12

- Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution
- Vol. 34, Issue 4, Pages: 364-369(2020)
- 作者机构：
  
  山西大学生命科学学院,太原,030006
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2020.04.052
  CLC：
- Published：2020
- 稿件说明：
移动端阅览
LIU Yuechang, LI Baozhen, WANG Tao, et al. Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution[J]. 2020, 34(4): 364-369.
DOI：

LIU Yuechang, LI Baozhen, WANG Tao, et al. Study of Two Microbes Combined to Remediate Field Soil Cadmium Pollution[J]. 2020, 34(4): 364-369. DOI： 10.13870/j.cnki.stbcxb.2020.04.052.

摘要

采用盆栽试验，运用BCR四步法、火焰原子吸收法和稀释涂布平板法等方法，研究了沼泽红假单胞菌（Rhodopseudanonas palustris）和枯草芽孢杆菌（Bacillus subtilis）联合作用对修复农田土壤重金属镉污染盆栽种植小白菜（Brassica campestris L.ssp.chinensis Makino）的效果及安全性，旨在探讨降低土壤镉生物有效性的有效途径，为新型生物菌肥研究提供理论依据。结果表明，沼泽红假单胞菌、枯草芽孢杆菌单独作用以及联合作用均能使农田土壤中镉的固定态增加，降低农田土壤中镉的生物有效性，联合菌处理组镉的生物有效性降低最显著，为32.70%，均对农田土壤中微生物各生理类群数量有显著性影响（P<0.05），其中，细菌的数量达到105 CFU/g；均显著降低了小白菜根、茎中镉含量，联合菌处理组根、茎中镉的去除率分别达到58.06%~71.37%和73.66%~76.70%；均增加了小白菜株高、茎鲜重、叶绿素a、b的含量和叶绿素a/b的比值，联合菌处理组小白菜的生物量增加18.13%~69.55%。研究结果说明，沼泽红假单胞菌和枯草芽孢杆菌联合作用，对农田土壤镉污染的修复效果显著，且促进了小白菜的生长，具有修复农田土壤重金属污染的潜力。

Abstract

This research used the potted plant approach

BCR four steps work

flame atomic absorption method

and the method of dilution coated tablet to study the joint action of Rhodopseudanonas palustris and Bacillus subtilis

as well as the efficacy and safety

for remediation of cadmium pollution in farmland soil by Chinese cabbage in order to explore its effectiveness and to provide theoretical basis for application of the new type bio-bacterial manure. The results showed that either R. palustris or B. subtilis alone

or their combination could enhance the fixation of the metal in soil by increasing insoluble state cadmium and decreasing cadmium bioavailability. The combination of two bacteria significantly reduced cadmium bioavailability by 32.70%. All bacteria treatments showed significant influence on the number of soil microorganisms (P<0.05)

and the amount of bacteria reached 105 CFU/g. The treatments with various microorganisms all increased the plant height and fresh weight of cabbage

the content of chlorophyll a and b

and the ratio of chlorophyll a/b

meanwhile reduced the content of cadmium in the root and stem of cabbage with removal rate of 58.06%~71.37% and 73.66%~76.70%

respectively. The combination treatment group had the highest remediating effect with the biomass of Chinese cabbage increased by 18.13%~69.55%. The results demonstrated that the combination of R. spalustris and B. subtilis had a strong ability to remove cadmium from contaminated soil

could significantly promote the growth of Chinese cabbage

and showed the potential for remediation of heavy metal pollution in farmland soil.

关键词

Keywords

references

Parnian A, Chorom M, Fard N J H. A potential method for remediation of Cadmium pollution in aquatic medium by hydrophyte,

Ceratophyllum demersum

L.[J].Majallah-i āb va Khāk,2017,30(6):1918-1929.

田喜强,赵宏吉,唐志国,等.土壤污染及修复方法的研究进展[J].现代农业科技,2019,738(4):162-163,167.

肖春文,罗秀云,田云,等.重金属镉污染生物修复的研究进展[J].化学与生物工程,2013,8(2):23-25.

俞纪翔.微生物在治理土壤重金属危害时的作用[J].世界有色金属,2018,513(21):180-181.

李婧,周艳文,陈森,等.我国土壤镉污染现状危害及其治理方法综述[J].安徽农学通报,2015,21(24):104-107.

Zhou N, Chen H G, Xi J T, et al. Biochars with excellent Pb (Ⅱ) adsorption property produced from fresh and dehydrated banana peels via hydrothermal carbonization[J].Bioresource Technology,2017,232:204-210.

Zhang Y L, Xu X J, Chen A M, et al. Maternal urinary cadmium levels during pregnancy associated with risk of sex-dependent birth outcomes from an e-waste pollution site in China[J].Reproductive Toxicology,2018,75:49-55.

姚竹云,张肇铭.几株光合细菌的表型特征及DNA-NDA同源性分析[J].应用与环境生物学报,1996,2(1):84-89.

叶碧霞,左勇,傅彬,等.一株枯草芽孢杆菌培养基及培养条件的优化[J].食品科技,2017,42(1):23-28.

范春辉,杜波,张颖超,等.湿法消解火焰原子吸收法测定黄土复合污染修复植物金盏菊幼苗中的铅和镉[J].光谱学与光谱分析,2016,36(8):2625-2628.

张永利,刘晓文,陈启敏,等.Tessier法和改进BCR法提取施加熟污泥后黄土中Cd的对比研究[J].环境工程,2019,37(5):34-38.

李晓洁.试论土壤中的重金属检测样品前处理技术[J].世界有色金属,2019(7):222-223.

胡秉芬,黄华梨,季元祖,等.分光光度法测定叶绿素含量的提取液的适宜浓度[J].草业科学,2018,35(8):1965-1974.

侯建伟,邢存芳,邓晓梅,等.花椒林下土壤微生物数量和酶活性对生物质炭的响应[J].西北农林科技大学学报(自然科学版),2020,48(4):89-96.

信艳杰,胡晓娟,曹煜成,等.光合细菌菌剂和沼泽红假单胞菌对实验水体氮磷营养盐和微生物群落的影响[J].南方水产科学,2019,15(1):31-41.

肖根林,白红娟,贾万利.光合细菌对土壤中Cd形态分布的影响[J].化工技术与开发,2011,40(3):43-45.

白红娟,肖根林,贾万利.光合细菌减少蔬菜中Pb、Cd及呋喃丹污染的研究[J].中国土壤与肥料,2013(2):94-99.

白红娟.光合细菌改善Cd、Pb及呋喃丹污染土壤的微生物群落DNA序列多样性的研究[J].环境污染与防治,2011,33(11):24-28.

李晓晴.枯草芽孢杆菌对花生镉积累的影响机制研究[D].济南:山东师范大学(山东省农业科学院试验场),2013.

王丽丽,杨谦.接种枯草芽孢杆菌和丛枝菌根真菌促进红三叶修复石油污染土壤[J].江苏农业科学,2016,44(5):526-529.

Dixit R, Wasiullah, Malaviya D, et al. Bioremediation of heavy metals from soil and aquatic environment:An overview of principles and criteria of fundamental processes[J].Sustainability,2015,7(2):2189-2212.

Paz-Ferreiro J, Lu H, Fu S, et al. Use of phytoremediation and biochar to remediate heavy metal polluted soils:A review[J].Solid Earth,2014,5(1):65-75.

唐文忠,孙柳,单保庆.土壤/沉积物中重金属生物有效性和生物可利用性的研究进展[J].环境工程学报,2019,13(8):1775-1790.

陈海涛.利用微生物肥料进行土壤生态修复治理的研究[J].化工管理,2019(3):192-193.

黄成涛,黄位权,史鼎鼎,等.一株耐铅镉真菌Q7对香根草吸收累积重金属的效应[J].应用与环境生物学报,2018,24(4):901-907.

Jiang Q Y, Zhuo F, Long S H, et al. Can arbuscular mycorrhizal fungi reduce Cd uptake and alleviate Cd toxicity of Lonicera japonica grown in Cd-added soils?[J].Scientific Reports,2016,6(1):21805.

Chai L Y, Huang S H, Yang Z H, et al. Cr (VI) remediation by indigenous bacteria in soils contaminated by chromium-containing slag[J].Journal of Hazardous Materials,2009,167(1/3):516-522.

Xu J B, Feng Y Z, Wang Y L, et al. Effect of

Rhizobacterium Rhodopseudomonas palustris

inoculation on stevia rebaudiana plant growth and soil microbial community[J].Pedosphere,2018,28(5):793-803.

Cao S M, Wang W K, Wang F, et al. Drought-tolerant

Streptomyces pactum

Act12 assist phytoremediation of cadmium-contaminated soil by

Amaranthus hypochondriacus

:Great potential application in arid/semi-arid areas[J].Environmental Science and Pollution Research,2016,23(15):14898-14907.

Views

1052

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Coated Slow-release Urea on the Chinese Cabbage Physiological Characteristics

Effects of Bacillus Subtilis on Water Movement and Water Stable Aggregate in Saline Alkali Soil

Effects of Different Levels Cd Stress on Cd Uptake and Physiological Response of Solanum nigrum L.

Effects of Bacillus subtilis on Evaporation of Soil Surface and Water and Salt Dsitritution in Saline-alkali Soil

Effect of Fish Bone Meal on Immobilization Remediation of Cadmium Contaminated Soil and Its Physiochemical Properties

Related Author

WANG Yan-hua

QIU Xian-kui

QU Chang-feng

曲长凤

ZHANG Min

LI Xu-hua

DONG Yuan-jie

陈晓鹏

Related Institution

DONG Yuan-jie1,LI Xu-hua1,ZHANG Min1,2,QU Chang-feng1,QIU Xian-kui1,WANG Yan-hua1,HU Guo-qing1(1.College of Resources and Environment,Shandong Agricultural University,Chinese National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources,Chinese National Engineering Research Center for Slow/Controlled Release Fertilizers,State Key Laboratory of Crop Biology,Taian,Shandong 271018,2.Shandong Kingenta Ecological Engineering Co.,Ltd.,Linshu,)

山东金正大生态工程股份有限公司

State Key Laboratory Base of Eco-hydraulic Engineering in Arid Area, Xi’an University of Technology

State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Yangling

Hunan Province Key Laboratory of Coal Resources Clean-utilization and Mine Environment Protection, Hunan University of Science and Technology

⁰