Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa

HUANG Ting; MA Dongmei; WANG Wenjing; ZHAO Lijuan; MA Qiaoli; CAI Jinjun

doi:10.13870/j.cnki.stbcxb.2020.02.031

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Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa

更新时间：2025-03-12

- Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa
- Vol. 34, Issue 2, Pages: 216-221(2020)
- 作者机构：
  
  1. 宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地, 西北退化生态系统恢复与重建教育部重点实验室,银川,750021
  2. 宁夏大学农学院,银川,750021
  3. 宁夏农林科学院农业资源环境研究所,银川,750021
  4. 西北农林科技大学资源与环境学院, 陕西杨凌,712100
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2020.02.031
  CLC： S541.9
- Published：2020
- 稿件说明：
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HUANG Ting, MA Dongmei, WANG Wenjing, et al. Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa[J]. 2020, 34(2): 216-221.
DOI：

HUANG Ting, MA Dongmei, WANG Wenjing, et al. Preliminary Study on Physiological Characteristics of Salt Tolerance of Two Genotypes of Alfalfa[J]. 2020, 34(2): 216-221. DOI： 10.13870/j.cnki.stbcxb.2020.02.031.

摘要

为研究2种紫花苜蓿（Medicago sativa L.） "阿迪娜" （耐盐基因型）和"秘鲁"（敏盐基因型）在盐胁迫处理下的生理特性与耐盐机理，采用150 mmol/L NaCl胁迫处理2种紫花苜蓿幼苗，分别测定盐处理前和处理后2，4，6，8，16 h的过氧化氢酶（CAT）、过氧化物酶（POD）、丙二醛（MDA）、脯氨酸（PRO）、相对含水量（RWC）和叶绿素（Chl）含量。结果表明：2种紫花苜蓿的过氧化氢酶活性、过氧化物酶活性和丙二醛含量在盐胁迫下总体呈上升趋势，脯氨酸含量和相对含水量表现出相反的趋势。叶绿素含量在盐胁迫前期稳定不变，但在胁迫后期显著性下降；2种紫花苜蓿的相对含水量和叶绿素含量的变化趋势与植株表型变化相对应。主成分分析表明，过氧化氢酶和丙二醛的贡献率最大，能更好地为苜蓿耐盐机理及分子育种研究提供理论依据。

Abstract

The physiological characteristics and salt tolerance mechanism of two genotypes of alfalfa (Medicago sativa L.) Adrenalin (salt-stress tolerant) and Peru (salt-stress sensitive) under salt stress were explored by using 150 mmol/L NaCl to deal with two genotypes of alfalfa seedlings. Catalase (CAT)

malondialdehyde (MDA)

peroxidase (POD)

relative water content (RWC)

Chlorophyll (Chl)

and proline (PRO) were respectively determined before and after 2

16 hour of salt stress. The result showed that the catalase activity

the peroxidase activity

and the malondialdehyde content of two genotypes of alfalfa showed a trend of increase under salt stress

while the proline content and the relative water content showed an opposite trend. The chlorophyll content significantly decreased. In addition

it was found that the variation of the relative water content and the chlorophyll content corresponded to the change of plant phenotype. Principal component analysis showed that the catalase and the malondialdehyde contributed the most

which could provide a theoretical basis for the research on salt tolerance mechanism and molecular breeding of alfalfa.

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