Alleviative Effects of Exogenous H

doi:10.13870/j.cnki.stbcxb.2019.06.051

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Alleviative Effects of Exogenous H

更新时间：2025-03-12

- Alleviative Effects of Exogenous H
- Vol. 33, Issue 6, Pages: 361-369(2019)
- 作者机构：
  
  1. 内蒙古农业大学沙漠治理学院,呼和浩特,010018
  2. 中国林业科学研究院沙漠林业实验中心, 内蒙古磴口,015200
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2019.06.051
  CLC：
- Published：2019
- 稿件说明：
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Alleviative Effects of Exogenous H[J]. 2019, 33(6): 361-369.
DOI：

Alleviative Effects of Exogenous H[J]. 2019, 33(6): 361-369. DOI： 10.13870/j.cnki.stbcxb.2019.06.051.

摘要

通过温室盆栽土培试验，研究不同浓度（0，0.02，0.04，0.06，0.08，0.1，0.3 mmol/L）的外源H2O2处理对0.05 mmol/L Cd胁迫下酸枣幼苗生长、光合系统和荧光特性等的影响。结果表明：（1）Cd胁迫下，酸枣幼苗生长受到抑制，经H2O2处理后，酸枣幼苗对镉抗性系数、光合绿素含量、净光合速率（Pn）、蒸腾速率（Tr）和气孔导度（Gs）均升高，过氧化氢（H2O2）、丙二醛（MDA）含量和胞间CO2浓度（Ci）则出现不同程度的下降；（2）低浓度H2O2（≤ 0.08 mmol/L）处理后，酸枣叶片和根系内抗氧化酶[过氧化物酶（POD）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）]活性增强，叶片内1，5-二磷酸核酮糖羧化酶（Rubisco）、1，1-二磷酸景天庚酮糖酯酶（SBPase）和1，6-二磷酸果糖醛缩酶（FBAase）活性最高显著上升38.24%，42.15%，84.08%，但转酮醇酶（TKase）活性无显著变化；（3）酸枣叶片内PSⅡ最大光化学效率（Fv/Fm）、实际光化学效率（ΦPSⅡ）、光化学猝灭系数（qP）和吸收光能用于光化学反应的份额（P）在H2O2处理下最大分别提高37.52%，135.95%，53.10%和98.36%，PSⅡ非光化学猝灭系数（NPQ）、调节性能量耗散Y（NPQ）、非调节性能量耗散Y（NO）、吸收光能用于天线热耗散的份额（D）、PSⅡ反应中心非光化学耗散的份额（Ex）和双光系统间激发能分配不平衡偏离系数（β/α-1）分别降低34.13%，35.15%，30.26%，35.52，32.30%和53.43%，缓解效果显著，但随着外源H2O2喷施浓度的增加，缓解效应有下降趋势。综合分析发现，H2O2对酸枣镉毒的缓解作用与其改善酸枣光合作用、维持抗氧化系统高活性和提高PSⅡ光化学效率等多种生理过程有关。其中以0.08 mmol/L H2O2提高酸枣的修复效率最佳，可作为植物修复的强化措施。

Abstract

Through a pot soil-culture experiment in greenhouse

the effects of exogenous H2O2 treatment with different concentrations (0

0.02

0.04

0.06

0.08

0.1

0.3 mmol/L) on the growth

photosynthetic system

and fluorescence characteristics of Z. jujube seedlings under the stress of 0.05 mmol/L Cd were studied. The results showed that:(1) Under Cd stress

the growth of Z. jujube seedlings was inhibited. After H2O2 treatment

the Cd resistance coefficient

photosynthetic chlorophyll content

net photosynthetic rate (Pn)

transpiration rate (Tr)

and stomatal conductance (Gs) of Z. jujube seedlings increased

while H2O2

malondialdehyde (MDA) content

and intercellular CO2 concentration (Ci) decreased with different degrees. (2) The activities of antioxidases (Peroxidase (POD)

superoxide dismutase (SOD)

and catalase (CAT)) in the leaves and roots of Z. jujube enhanced after treatment with low concentration of H2O2 (≤ 0.08 mmol/L)

and the activities of ribulose-1

5-bisphosphate carboxylase/oxygenase (Rubisco)

sedoheptulose-1

1-bisphosphatase (SBPase)

and fructose 1

6-diphosphate aldolase (SBPase) in the leaves increased significantly by 38.24%

42.15%

and 84.08%

respectively. However

there was no significant change for the activity of transketolase (TKase). (3) The maximal photochemistry efficiency (Fv/Fm)

actual photochemistry efficiency (ΦPSⅡ)

photochemical quenching coefficient (qP)

and the share of absorbed light energy for photochemical reactions (P) in the leaves of Z. jujube increased by 37.52%

135.95%

53.10%

and 98.36% respectively under the treatment of H2O2. The PSⅡ non-photochemical quenching coefficient (NPQ)

regulatory energy dissipation Y (NPQ)

non-regulatory energy dissipation Y (NO)

the proportion of absorbed light energy used for antenna heat dissipation (D)

the proportion of non-photochemical dissipation of PS II reaction center (Ex)

and the unbalanced deviation coefficient of excitation energy distribution between two optical systems (β/α-1) decreased by 34.13%

35.15%

30.26%

35.52

32.30%

and 53.43%

respectively. The mitigate effect was significant

but the mitigation effect tended to decline with the increase of exogenous concentration of H2O2. Comprehensive analysis showed that the mitigate effect of H2O2 on cadmium toxicity of Z. jujube was related to many physiological processes

such as improving photosynthesis of Z. jujube

maintaining the high activity of antioxidant system and improving photochemical efficiency of PS II. Among them

0.08 mmol/L H2O2 was the best way to improve the repair efficiency of Z. jujube

which could be used as a strengthening measure of phytoremediation.

关键词

Keywords

references

郑黎明,张杰,杨红飞,等.镉胁迫对荻生长、镉富集和土壤酶活性的影响[J].水土保持学报,2017,31(5):334-339.

张美玲,廖伟彪,肖洪浪.一氧化氮和过氧化氢对万寿菊不定根形成的影响[J].中国沙漠,2012,32(1):105-111.

An G Y, Li B Z, Wu G L, et al. H

could act as root source signal to mediate stomatal closure induced by salt stress of

Vicia fafa

L.[J].Plant Physiology Journal,2012,48(3):265-271.

王琳,陈奇,武孔焕,等.过氧化氢预处理增强敏感型黑豆抗铝能力的生理机制[J].西北植物学报,2013,33(2):336-342.

杨锐,郎莹,张光灿,等.野生酸枣光合及叶绿素荧光参数对土壤干旱胁迫的响应[J].西北植物学报,2018,38(5):922-931.

朱广龙,邓荣华,魏学智.酸枣叶表皮微形态对不同生态环境的适应特征[J].生态学报,2016,36(16):5193-5203.

李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.

Giannopolitis C N, Ries S K. Superoxide dismutases:I.Occur-rence in higher plants[J].Plant Physiology,1977,59(2):309.

Jiang J L, Su M, Wang L Y, et al. Exogenous hydrogen peroxide reversibly inhibits root gravitropism and induces horizontal curvature of primary root during grass pea germination[J].Plant Physiology and Biochemistry,2012,53:84-93.

陈建勋,王晓峰.植物生理学实验指导[M].2版.广州:华南理工大学出版社,2006.

Rao I M, Terry N. Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet:I. changes in growth, gas exchange, and calvin cycle enzymes[J].Plant Physiology,1989,90(3):814-819.

Braun G, Malkin S. Regulation of the imbalance in light excitation between photosystem II and photosystem I by cations and by the energized state of the thylakoid membrane[J].Biochemica et Biophysica Acta(BBA)-bioenergetics,1990,1017(1):79-90.

Demmig-Adams B, Adams III W W, Barker D H, et al. Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation[J].Physiologia Plantarum,1996,98(2):253-264.

周蜜,吴玉环,刘星星,等.镉胁迫对菊芋生理变化及镉富集的影响[J].水土保持学报,2019,33(2):323-330.

王佳星,余国源,谢瑛,等.土壤镉胁迫对紫金牛生理特性的影响[J].东北林业大学学报,2019,47(5):25-29.

杨波,何俊瑜,任艳芳,等.过氧化氢对镉胁迫下水稻种子萌发的缓解效应[J].植物生理学报,2018,54(6):1111-1118.

李雪萍,金鑫,廖伟彪,等.过氧化氢参与干旱条件下脱落酸诱导黄瓜不定根形成[J].干旱地区农业研究,2018,36(3):194-201.

耿兴敏,肖丽燕,赵晖,等.H

预处理及高温胁迫下杜鹃叶片活性氧及抗氧化酶亚细胞定位分析[J].西北植物学报,2019,39(5):791-800.

Andre'Dias de A N, Jose'Tarquinio P, Joa-quim Ene'as-Filho, et al. Hydrogen peroxide pre-treatment induces salt stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162:1114-1122.

郭智,原海燕,奥岩松.镉胁迫对龙葵幼苗光合特性和营养元素吸收的影响[J].生态环境学报,2009,18(3):824-829.

王艳玲.H

处理在水稻响应镉胁迫中的作用机制初探[D].贵阳:贵州大学,2017.

Cho U H, Seo N H. Oxidative stress in

Arabidopsis thaliana

exposed to cadmium is due to hydrogenperoxide accumulation[J].Plant Science,2005,168(1):113-120.

Wahid A, Perveen M, Gelani S, et al. Pretreatment of seed with H

improves salt tolerance of wheet seedlings by alleviation of oxidative damage and expression of stress proteins[J].Journal of Plant Physiology,2007,164(3):283-294.

Fedina S, Nedeva D, Click N. Pre-treatment with H

induces salt tolerance in Barley seedlings[J].Biologia Plantarum,2009,53(2):321-324.

Azevedo N A D, Prisco J T, Enéas-filho J, et al. Hydrogen peroxide pre-treatment induces salt-stress acclimation in maize plants[J].Journal of Plant Physiology,2005,162(10):1114-1122.

潘晨慧.交替途径及外源过氧化氢在唐古特白刺耐盐性中的作用[D].哈尔滨:东北农业大学,2018.

Tang Z H, Yang L, Zu Y G, et al. Variations of vinblastine accumulation and redox state affected by exogenous H

Catharanthus roseus

(L.) G. Don[J].Plant Growth Regulation,2009,57(1):15-20.

胡化广,张振铭,季芳芳,等.干旱胁迫对结缕草叶绿素荧光和光合关键酶活性的影响[J].草地学报,2016,24(6):1304-1308.

李翔,桑勤勤,束胜,等.外源油菜素内酯对弱光下番茄幼苗光合碳同化关键酶及其基因的影响[J].园艺学报,2016,43(10):2012-2020.

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