Regulation of Si/As on Arsenic Uptake, Efflux and Translocation in Rice Seedlings (

FU Jie; CHEN Miaomiao; FAN Limin; SUN Hongxin; XUE Peiying; LIU Wenju

doi:10.13870/j.cnki.stbcxb.2019.04.052

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Regulation of Si/As on Arsenic Uptake, Efflux and Translocation in Rice Seedlings (

更新时间：2025-03-12

- Regulation of Si/As on Arsenic Uptake, Efflux and Translocation in Rice Seedlings (
- Vol. 33, Issue 4, Pages: 370-376(2019)
- 作者机构：
  
  1. 河北农业大学资源与环境科学学院, 河北省农田生态环境重点实验室,河北,保定,071000
  2. 河北农业大学科学技术研究院,河北,保定,071001
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2019.04.052
  CLC： X53;S511.2
- Published：2019
- 稿件说明：
移动端阅览
FU Jie, CHEN Miaomiao, FAN Limin, et al. Regulation of Si/As on Arsenic Uptake, Efflux and Translocation in Rice Seedlings ([J]. 2019, 33(4): 370-376.
DOI：

FU Jie, CHEN Miaomiao, FAN Limin, et al. Regulation of Si/As on Arsenic Uptake, Efflux and Translocation in Rice Seedlings ([J]. 2019, 33(4): 370-376. DOI： 10.13870/j.cnki.stbcxb.2019.04.052.

摘要

通过生物模拟法，将水稻幼苗分别暴露于含有10 μmol/L无机三价砷As （Ⅲ）或五价砷As （V）的营养液中12，24，48 h，探究硅砷摩尔比（Si/As为0∶1，100∶1和200∶1）对苗期水稻吸收、外排、转运和累积砷的影响。结果表明，水稻暴露于含As （Ⅲ）营养液中12 h，与Si/As为0∶1相比，Si/As为200∶1的处理使水稻的As （Ⅲ）吸收速率降低30.7%（P<0.05），且随着暴露时间的延长，水稻对As （Ⅲ）的吸收速率逐渐降低，不同Si/As对其吸收速率的影响也减弱。处理48 h，100∶1和200∶1的Si/As均降低水稻根部As （Ⅲ）向茎叶的转移系数，较Si/As为0∶1分别降低51.2%和56.9%，同时水稻地上部As （Ⅲ）含量比Si/As为0∶1的处理分别降低50.7%和67.2%；暴露在As （V）营养液中12 h，Si/As为100∶1促进水稻对As （V）的吸收，增幅高达82.3%，但暴露24 h，Si/As为200∶1则抑制水稻根系对As （V）的吸收，以及As （Ⅲ）的外排和As （Ⅲ）由水稻根系向茎叶的转运，降幅分别为28.0%，41.9%和39.9%。暴露48 h时，Si/As为200∶1的水稻As （Ⅲ）转移系数较Si/As为100∶1的处理降低57.9%，并且处理24，48 h时，Si/As为100∶1和200∶1水稻地上部As （Ⅲ）含量显著降低53.6%和75.0%，25.0%和52.8%。此外，水稻根系对As （V）的吸收与As （Ⅲ）的外排之间呈极显著正相关关系（r=0.921，P<0.01），该关系不受Si/As和暴露时间的影响。无论是As （Ⅲ）或As （V）处理，在12~48 h的暴露时间内，Si/As为200∶1显著抑制砷的吸收、As （Ⅲ）的外排和As （Ⅲ）在水稻体内的转运。

Abstract

A hydroponic experiment was carried out to explore the effects of Si/As ratios (0:1

100:1 and 200:1) on the uptake

efflux

translocation and accumulation of arsenic in rice seedlings that were grown in nutrient solution containing 10 μmol/L arsenite or arsenate for 12

48 h. The results showed that Si/As of 200:1 reduced arsenite uptake rate compared to Si/As of 0:1

accounting for 30.7% (P<0.05)

when rice was exposed to nutrient solution with arsenite and silicon for 12 h. The uptake rates of arsenite of rice gradually decreased and the effect of different Si/As ratios reduced with the prolongation of exposure time. Furthermore

compared to Si/As of 0:1

the ratios of Si/As at 100:1 and 200:1 inhibited the translocation of arsenite from roots to shoot and the concentrations of As(Ⅲ) were decreased by 51.2% and 56.9%

respectively

when rice seedlings exposed to nutrient solution with arsenite for 48 h

at the same time

the concentrations of As(Ⅲ) in rice shoots were significantly lower than Si/As of 0:1

accounting for 50.7% and 67.2%

respectively. When rice seedlings were exposed to arsenate nutrient solution for 12 h

Si/As of 100:1 promoted the uptake rate of arsenate by rice

which was 82.3% higher than Si/As of 0:1. However

the ratio of Si/As of 200:1 inhibited the uptake rates of arsenate

arsenite efflux by rice roots and translocation of As(Ⅲ) after 24 h for exposure to asenate

accounting for 28.0%

41.9% and 39.9%

respectively. The translocation of As(Ⅲ) from root to shoot with Si/As of 200:1 was 57.9% lower than that of Si/As of 100:1 after 48 h of exposure time. In addition

the concentrations of As(Ⅲ) in rice shoots in the treatment of Si/As ratio at 100:1 and 200:1 for 24 h or 48 h were significantly lower than the control

accounting for 53.6% and 75.0%

25.0% and 52.8%. In addition

there was a significant positive correlation between the uptake of As(V) and the efflux of As(Ⅲ) in rice roots (r=0.921

P<0.01)

which was not affected by Si/As and exposure time. In summary

when rice seedlings were exposed in nutrient solution with As(Ⅲ) or As(V) for 12~48 h

the ratio of Si/As at 200:1 significantly inhibited arsenic uptake

As(Ⅲ) efflux and translocation of As(Ⅲ) from root to shoot in rice.

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