Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of

ZHANG Tingting; ZHU Yexinyi; WU Yuhuan; ZHANG Qi; YE Run; CUI Leyi; CHEN Rui; TAO Tao; LIU Peng

doi:10.13870/j.cnki.stbcxb.2019.04.047

您当前的位置：

首页 >

文章列表页 >

Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of

更新时间：2025-03-12

- Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of
- Vol. 33, Issue 4, Pages: 335-341(2019)
- 作者机构：
  
  1. 浙江师范大学植物学实验室,浙江,金华,321004
  2. 杭州师范大学生命与环境科学学院,杭州,310036
  3. 中国科学院沈阳应用生态研究所,沈阳,110016
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2019.04.047
  CLC： Q945.78;S567.9
- Published：2019
- 稿件说明：
移动端阅览
ZHANG Tingting, ZHU Yexinyi, WU Yuhuan, et al. Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of[J]. 2019, 33(4): 335-341.
DOI：

ZHANG Tingting, ZHU Yexinyi, WU Yuhuan, et al. Effects of Exogenous IAA on Physiological Changes and Cadmium Tolerance of[J]. 2019, 33(4): 335-341. DOI： 10.13870/j.cnki.stbcxb.2019.04.047.

摘要

为探究IAA对镉胁迫下栝楼（Trichosanthes kirilowii Maxim.）生理响应及耐镉性的影响，以保定栝楼（耐镉品种）和浦江栝楼（镉敏感品种）为材料，进行土培试验，研究800 μmol/L镉胁迫下不同浓度IAA （10，35，60 μmol/L）对2个栝楼品种的生长特性、光合特性、抗氧化酶活性及丙二醛（MDA）含量的影响。结果表明：（1）与CK组相比，800 μmol/L Cd处理下，保定和浦江栝楼的平均根长、地下部分鲜重、超氧化物歧化酶（SOD）活性、叶绿素含量、最大光化学效率（Fv/Fm）和电子传递速率（ETR）明显降低，初始荧光（Fo）、过氧化物酶（POD）、过氧化氢酶（CAT）活性及MDA含量显著增高，细胞膜脂过氧化程度加深；（2）随IAA浓度升高，保定栝楼平均根长与地下部分鲜重持续增长，最大增幅为24.83%和40.55%，而浦江栝楼则由上升转为下降趋势；（3）外源IAA加入后，2个栝楼品种幼叶叶绿素a、叶绿素b、叶绿素a+b含量、叶绿素a/b、叶绿素荧光参数中的Fv/Fm、ETR及抗氧化酶SOD、POD、CAT活性均呈现出先升后降的趋势，Fo和MDA含量先降后升，其中，35 μmol/L的IAA使叶绿素a、叶绿素b、叶绿素a+b含量、叶绿素a/b、Fv/Fm、ETR及SOD、CAT活性上升到峰值，而MDA含量降低35.44%~43.23%，达到谷值；（4）由隶属函数均值耐镉性分析得知，不同浓度IAA对栝楼镉毒害的缓解程度为35 μmol/L > 10 μmol/L > 60 μmol/L > 0 μmol/L。由此可见，外源IAA的施加对于缓解植物镉胁迫具有一定的应用前景。

Abstract

In order to investigate the effects of IAA on physiological response and cadmium tolerance of Trichosanthes kirilowii Maxim. under cadmium stress

Baoding T. kirilowii (cadmium-tolerant) and Pujiang T. kirilowii were selected as experimental materials

and soil culture experiments were conducted to determine the effects of exogenous IAA (10

35 and 60 μmol/L) on physiological changes

photosynthetic characteristics

antioxidant enzyme activity and malondialdehyde (MDA) content of these two varieties under 800 μmol/L cadmium stress. The results showed that:(1) Compared with the CK

the average root length

fresh weight of underground part

superoxide dismutase (SOD) activity

chlorophyll content

maximum photochemical efficiency (Fv/Fm) and electron transfer rate (ETR) of the two kinds of T. kirilowii significantly decreased under 800 μmol/L Cd treatment

while the initial fluorescence (Fo)

peroxidase (POD) activity

catalase (CAT) activity and MDA content increased significantly

and the lipid peroxidation of cell membrane deepened. (2) With the increasing of IAA concentration

the average root length and fresh weight of underground part of Baoding T. kirilowii increased continuously

with the maximum increase of 24.83% and 40.55%

while those of Pujiang T. kirilowii changed from ascending to descending. (3) After the addition of IAA

the content of chlorophyll a

a+b and chlorophyll a/b

Fv/Fm

ETR

SOD

POD and CAT activities in the young leaves of the two varieties increased first and then decreased

while Fo and MDA content dropped firstly and then increased. The content of chlorophyll a

a+b

a/b

Fv/Fm

ETR

SOD and CAT increased to the peak values with the addition of IAA at 35 μmol/L

while MDA decreased by 35.44%~43.23%

reaching the valley value. (4) The analysis of membership function indicated that the ability of cadmium tolerance of different IAA concentrations was 35 μmol/L > 10 μmol/L > 60 μmol/L > 0 μmol/L. Therefore

applying exogenous IAA has a certain application prospect in alleviating cadmium stress in plants.

关键词

Keywords

references

许文,吴献,黄鸣清,等.栝楼桂枝汤的血清药物化学研究[J].中草药,2017,48(10):2033-2043.

臧畅,吕志强,董莲春,等.不同性别桑树幼苗对镉与酸雨复合处理的生长响应及镉积累差异[J].应用生态学报,2018,29(3):969-975.

龙小林,向珣朝,徐艳芳,等.镉胁迫下籼稻和粳稻对镉的吸收、转移和分配研究[J].中国水稻科学,2014,28(2):177-184.

谢惠玲,陈爱萍,张凤英,等.紫苏对不同浓度镉胁迫的生理响应[J].中国生态农业学报,2011,19(3):672-675.

刘赛.土壤镉有效性与水稻镉含量的相关性分析[D].长沙:湖南师范大学,2016.

Singh S, Prasad S M. IAA alleviates Cd toxicity on growth, photosynthesis and oxidative damages in eggplant seedlings[J].Plant Growth Regulation,2015,77(1):87-98.

Bashri G, Prasad S M. Exogenous IAA differentially affects growth, oxidative stress and antioxidants system in Cd stressed

Trigonella foenum-graecum

L. seedlings:Toxicity alleviation by up-regulation of ascorbate- glutathione cycle[J]. Ecotoxicology and Environmental Safety,2016,132:329-338.

任桂萍,王小菁,朱根发.不同光质的LED对蝴蝶兰组织培养增殖及生根的影响[J].植物学报,2016,51(1):81-88.

姚虹宇,刘亚敏,张盛楠,等.外源柠檬酸对铝胁迫下马尾松生理特性的影响[J].林业科学,2018,54(7):155-164.

许红梅,李进,张元明.水分条件对人工培养齿肋赤藓光化学效率及生理特性的影响[J].植物生态学报,2017,41(8):882-893.

刘兆新,刘妍,杨坚群,等.小麦花生一体化施肥对麦套花生生理特性及产量的影响[J].水土保持学报,2018,32(1):344-351.

Chen L, Han Y, Jiang H, et al. Nitrogen nutrient status induces sexual differences in responses to cadmium in

Populus

yunnanensis

. Journal of Experimental Botany,2011,62(14):5037-5050.

闫玉静,白健,付春霞,等.根域蓄水调控对苹果叶片光合特性和抗氧化酶活性的影响[J].园艺学报,2015,42(5):817-825.

李雪凝,董守坤,刘丽君,等.干旱胁迫对春大豆超氧化物歧化酶活性和丙二醛含量的影响[J].中国农学通报,2016,32(15):93-97.

Shi Q Y, Wang J R, Zou J H, et al. Cadmium localization and its toxic effects on root tips of barley[J]. Zemdirbyste Agriculture,2016,103(2):151-158.

杨波,何俊瑜,任艳芳,等.过氧化氢对镉胁迫下水稻种子萌发的缓解效应[J].植物生理学报,2018,54(6):1111-1118.

冯汉青,杜变变,王庆文,等.镉胁迫下活性炭对小麦幼根的保护作用[J].生态学报,2016,36(10):2962-2968.

梁虹,喻富根.1-萘氨甲酰苯甲酸、外源IAA和GA

对拟南芥根的伸长和向重力性反应的影响[J].植物生理学通讯,2007,43(4):653-656.

徐小逊,董袁媛,邓玉兰,等.镉胁迫对豨莶生长及光合作用相关参数的影响[J].农业环境科学学报,2016,35(9):1672-1679.

朱建玲,徐志防,曹洪麟,等.镉对南美蟛蜞菊光合特性的影响[J].生态环境,2008,17(2):657-660.

何梨香,黄运湘,黄楚瑜,等.圆叶决明对镉胁迫的生理响应[J].草业学报,2016,25(2):198-204.

陈晶.生长素类物质对镉胁迫下玉米幼苗生长及生理特性的影响[D].重庆:西南大学,2016.

黄红英,邓斌,苏丽娟,等.黄体素和β-胡萝卜素清除超氧阴离子和羟自由基能力的比较[J].植物生理学通报,2008,44(3):445-448.

吴永波,叶波.高温干旱复合胁迫对构树幼苗抗氧化酶活性和活性氧代谢的影响[J].生态学报,2016,36(2):403-410.

李亚萍,彭燕.IAA改善PEG处理下白三叶幼苗叶片抗氧化保护和渗透调节能力[J].草业科学,2017,34(11):2295-2302.

李丰涛.红麻对重金属的吸收特征及外源GSH缓解镉毒的机理研究[D].福州:福建农林大学,2013.

朱振华.施用吲哚乙酸对不同玉米品种幼苗镉吸收和生理生化的影响[D].昆明:昆明理工大学,2018.

Views

1044

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Different Light Intensities on Photosynthetic Characteristics of Invasive Species Bidens frondosa Leaves

Response of Photosynthetic Physiology and Yield of Spring Wheat to Drought-Rewatering

Effects of Brackish Water with Different Sodium-Potassium Ratios on Soil Water-Salt Characteristics and Winter Wheat Growth

Forms of Aluminum in Rhizosphere Soil and Its Effect on Growth of Fagopyrum esculentum and Fagopyrum cymosum

Growth and Antioxidant Responses of Lettuce Subjected to Combined Pollution of Simulated Acid Rain and Nickel in Red Soil

Related Author

LIAO Da

LI Binling

SONG Wei

ZHOU Bing

SU Qitao

YAN Xiaohong

YIN Haojie

WANG Rongrong

Related Institution

Key Laboratory of Jiangxi Province for Biological Invasion and Biosecurity， Ji′an

College of Life Science， Jinggangshan University， Ji′an

Agricultural College, Shihezi University

Institute for Soil and Water Conservation, Chinese Academy of Science and Ministry of Water Resources, Yangling

Institute of Soil and Water Conservation, Northwest A&F University, Yangling

⁰