Effects of PEG Pretreatment on Physiological Metabolism of

XIAN Jingping; WANG Yong; ZHANG Jiayang

doi:10.13870/j.cnki.stbcxb.2019.03.052

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Effects of PEG Pretreatment on Physiological Metabolism of

更新时间：2025-03-12

- Effects of PEG Pretreatment on Physiological Metabolism of
- Vol. 33, Issue 3, Pages: 358-364(2019)
- 作者机构：
  
  1. 新乡学院生命科学技术学院,河南,新乡,453000
  2. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心,兰州,730070
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2019.03.052
  CLC：
- Published：2019
- 稿件说明：
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XIAN Jingping, WANG Yong, ZHANG Jiayang. Effects of PEG Pretreatment on Physiological Metabolism of[J]. 2019, 33(3): 358-364.
DOI：

XIAN Jingping, WANG Yong, ZHANG Jiayang. Effects of PEG Pretreatment on Physiological Metabolism of[J]. 2019, 33(3): 358-364. DOI： 10.13870/j.cnki.stbcxb.2019.03.052.

摘要

为了探讨PEG预处理对盐胁迫和镉胁迫下多年生黑麦草幼苗生理特性的影响，将黑麦草幼苗分别用0，5%，10%，15%，20%，25%（对应水势分别为0，-0.05，-0.15，-0.30，-0.50，-0.77 MPa）的PEG-6000营养液进行预处理后，分别用含150 mmol/L NaCl和Cd2+浓度为10 mg/L的胁迫液培养，然后测定黑麦草幼苗叶片的光合色素含量、MDA含量、游离脯氨酸含量、可溶性糖含量及抗氧化酶（SOD、POD、CAT、APX）活性。结果表明：盐胁迫下15%（-0.30 MPa）PEG预处理和镉胁迫下10%（-0.15 MPa）PEG预处理可以有效提高多年生黑麦草的光合色素含量，降低MDA、游离脯氨酸含量，增加可溶性糖含量，提高抗氧化酶活性。PEG预处理下多年生黑麦草在遭受逆境胁迫时，受到多种生理生化的调节，其生理指标的动态变化是黑麦草应答逆境因子胁迫的重要调节机制，体现了其对逆境胁迫的适应能力以及在多种逆境胁迫下的交叉适应能力。

Abstract

Sand culture experiment were carried out to study the effects of PEG pretreatment on physiological characteristics of Lolium perenne seedlings under salt stress and cadmium stress. The experimental seedlings were dealt with 0%

10%

15%

20% and 25% (the corresponding water potential was 0

-0.05

-0.15

-0.30

-0.50 and -0.77 MPa

respectively) PEG-6000 pretreatment nutrient solutions

respectively

and then the seedlings were cultured separately by stress solutions containing 150 mmol/L NaCl and 10 mg/L Cd2+

respectively. Then the leaf chlorophyll(Chl)content

activities of antioxidant enzyme (such as catalase (CAT)

superoxide dismutase (SOD)

guaiacol peroxidase (POD) and ascorbate peroxidase (APX))

contents of osmoregulation substances

such as proline

soluble sugar and malondialdehyde (MDA) in leaves were determined. The test results showed that the 15% (-0.30 MPa) PEG pretreatment under the salt stress and the 10%(-0.15 MPa) PEG pretreatment under the Cd2+ stress could effectively increase the leaf chlorophyll contents of L. perenne seedlings

reduce the contents of MDA and proline

increase the content of soluble sugar and increase the activities of antioxidant enzymes. The above results showed that the L. perenne dealt by PEG pretreatment under stress was regulated by various physiological and biochemical factors. The dynamic changes of physiological indexes were important regulatory mechanisms for L. perenne in respons to stress

which reflected its adaptability to stress and its cross-adaptation ability under various stresses.

关键词

Keywords

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