Effects of Different Substrates on Physiological Characteristics of Maize Seedlings in Tetracycline Soil

SONG Jianli; QIN Junmei; XIONG Huaye; ZHANG Jian; NI Yue

doi:10.13870/j.cnki.stbcxb.2019.02.052

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Effects of Different Substrates on Physiological Characteristics of Maize Seedlings in Tetracycline Soil

更新时间：2025-03-12

- Effects of Different Substrates on Physiological Characteristics of Maize Seedlings in Tetracycline Soil
- Vol. 33, Issue 2, Pages: 342-349(2019)
- 作者机构：
  
  山西农业大学资源环境学院, 山西农业大学农业资源与环境国家级实验教学示范中心, 山西太谷,030801
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2019.02.052
  CLC：
- Published：2019
- 稿件说明：
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SONG Jianli, QIN Junmei, XIONG Huaye, et al. Effects of Different Substrates on Physiological Characteristics of Maize Seedlings in Tetracycline Soil[J]. 2019, 33(2): 342-349.
DOI：

SONG Jianli, QIN Junmei, XIONG Huaye, et al. Effects of Different Substrates on Physiological Characteristics of Maize Seedlings in Tetracycline Soil[J]. 2019, 33(2): 342-349. DOI： 10.13870/j.cnki.stbcxb.2019.02.052.

摘要

为探究兽用四环素对玉米幼苗生理特性的影响，通过玉米盆栽试验，研究四环素外源污染胁迫下施用不同基质（单施蚯蚓粪、菌糠、菌剂、生物炭及其与菌剂配施）对玉米生长的影响。结果表明，四环素对玉米生物量有明显的抑制作用，其中对根长的抑制作用显著大于株高，而添加不同基质均能缓解四环素对玉米株高和根长的抑制作用，且JK和JJ处理效果最显著，升幅分别为17.29%和30.08%；四环素显著诱导玉米SOD活性上升，升幅为3.34%，抑制CAT和POD活性，降幅分别为10.98%和46.68%，添加不同基质处理中，QY处理对促进抗氧化酶系统平衡的效果最显著；四环素会增加玉米脯氨酸、丙二醛、可溶性糖、可溶性蛋白及总黄酮含量，添加不同基质处理中，对其减少效果最显著的依次为JK、JJ、QY、S和JK处理，降幅分别为29.09%，50.88%，42.01%，50.23%和35.79%；相关性分析表明，脯氨酸、丙二醛均与可溶性蛋白和总黄酮呈极显著正相关，POD活性与可溶性糖、丙二醛与总黄酮相关性最强。综上所述，添加不同基质可有效缓解四环素对玉米幼苗的毒害作用，总趋势为JK （菌糠）、JJ （菌糠+菌剂）和QY （蚯蚓粪）处理效果较好。通过此研究，可筛选有效减少四环素对植物生长影响的基质及其施用方式，可为四环素对植物生理毒性的研究提供参考，也可为抗生素对植物的风险评价提供科学依据。

Abstract

In order to explore the effects of veterinary tetracycline on physiological characteristics of maize seedlings

pot experiment was conducted to study the effects of different substrates (single application of worm cast

fungal chaff

microbial inoculum

biochar and its combined application with microbial inoculum) on growth of maize seedlings under the stress of exogenous tetracycline pollution. The results showed that tetracycline inhibited significantly the maize biomass

and its inhibitory effect on root length was significantly greater than that on plant height. The inhibitory effect of tetracycline on maize plant height and root length could be alleviated by adding different substrates

and the effect of JK (fungal chaff) and JJ (fungal chaff + microbial inoculum) treatment was the most significant

with the increase of 17.29% and 30.08%

respectively. Tetracycline significantly increased SOD activity in maize by 3.34%

and inhibited CAT and POD activity by 10.98% and 46.68%

respectively. Among the treatments with different substrates

QY (worm cast) treatment had the most significant effect on promoting antioxidant enzyme system balance. Tetracycline could increase the content of proline

malondialdehyde

soluble sugar

soluble protein and total flavonoids in maize

among the treatments with different substrates

S and JK treatments had the most significant reduction effects

the decline rates were 29.09%

50.88%

42.01%

50.23% and 35.79%

respectively. Correlation analysis showed that proline and malondialdehyde both were extremely significantly positively correlated with soluble protein and total flavonoids

POD activity had the strongest correlation with soluble sugar

malondialdehyde had the strongest correlation with total flavonoids. In conclusion

adding different substrates could effectively alleviate the toxic effect of tetracycline on maize seedlings. The general trend showed that JK

JJ and QY had the best effect. Through this study

we could select the best substrates and application methods which could effectively reduce the effects of tetracycline on plant growth

provide reference for the research of physiological toxicity of tetracycline to plants

and also provide scientific basis for the risk assessment of antibiotic on plants.

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