Effect of As Stress on Millet Germination, Seedling Growth and Antioxidant System

WANG Chunmei; YAN Shuangdui; BU Yushan; LIU Lijun; ZHANG Naiyu

doi:10.13870/j.cnki.stbcxb.2018.06.051

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Effect of As Stress on Millet Germination, Seedling Growth and Antioxidant System

更新时间：2025-03-12

- Effect of As Stress on Millet Germination, Seedling Growth and Antioxidant System
- Vol. 32, Issue 6, Pages: 352-358(2018)
- 作者机构：
  
  1. 山西农业大学资源环境学院, 山西太谷,030801
  2. 山西农业大学生物工程研究所, 山西太谷,030801
  3. 山西省环境科学研究院,太原,030027
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2018.06.051
  CLC： S515;O613.63
- Published：2018
- 稿件说明：
移动端阅览
WANG Chunmei, YAN Shuangdui, BU Yushan, et al. Effect of As Stress on Millet Germination, Seedling Growth and Antioxidant System[J]. 2018, 32(6): 352-358.
DOI：

WANG Chunmei, YAN Shuangdui, BU Yushan, et al. Effect of As Stress on Millet Germination, Seedling Growth and Antioxidant System[J]. 2018, 32(6): 352-358. DOI： 10.13870/j.cnki.stbcxb.2018.06.051.

摘要

为探究As对谷子苗期生长的影响及谷子生长的临界浓度，设置As浓度梯度分别为0，2，4，8，10 mg/kg，以石英砂为基质，通过培养试验，分析探讨不同浓度As对谷子萌发、苗期生长与抗氧化酶系统的影响。研究表明：低浓度的As（<4 mg/kg）促进谷子萌发，高浓度的As（≥4 mg/kg）抑制谷子萌发。当As浓度为2 mg/kg时，谷子的发芽势、发芽率和发芽指数在所有处理中最高，其值分别为18.67%，32.00%和19.00%。低浓度的As对谷子幼苗生长有促进作用，高浓度对其有抑制作用，当As浓度为2 mg/kg，谷子的株高、根长和地上部鲜重与地下部鲜重都达到最大值，分别为15.46，7.34 cm和0.64，0.16 g，随着As浓度梯度增加，地下部鲜重/地上部鲜重先增加后降低，As对根系的促进与抑制作用大于地上部，谷子幼苗根部的As含量均明显高于地上部，且TF值逐渐减小；当As浓度<4 mg/kg时，As能促进谷子叶绿素的合成，在As浓度为2 mg/kg时，谷子的叶绿素a、叶绿素b和叶绿素总量显著高于对照28.38%，51.60%，37.06%，As浓度高于4 mg/kg时，谷子的叶绿素合成受到抑制；随着As浓度梯度的增加，谷子幼苗的SOD活性不断增强，POD与CAT活性呈先增加后减小的趋势，但均高于对照，As浓度>4 mg/kg后，CAT活性开始减小；As浓度>8 mg/kg后，谷子幼苗的POD活性开始减小，表明低浓度的As（<4 mg/kg）对谷子萌发及幼苗生长有促进作用，高浓度的As（≥4 mg/kg）抑制谷子萌发和幼苗生长，且根系对As胁迫的反应更为敏感，抗氧化酶系统（POD和CAT）活性降低。研究可为谷子的抗污染栽培和无公害生产提供科学依据。

Abstract

To investigate the effect of arsenic on the growth of millet seedlings and the critical concentration of millet stress

the As concentrations were set to 0

10 mg/kg for quartz sand. Substrates

through cultivation and pot experiments

analyzed the effect mechanism of As on the germination

seedling growth and antioxidant enzyme system of millet. Studies have shown that:Low concentrations of As (<4 mg/kg) can promote millet germination

high concentrations of As (<4 mg/kg) inhibit millet germination

when the As concentration of 2 mg/kg

the millet germination potential

germination rate and germination index in all treatments the highest value. They were 18.67%

32.00%

and 19.00%

respectively. The low concentration of As promoted the growth of millet seedlings

and the high concentration had an inhibitory effect. When the As concentration was 2 mg/kg

the plant height

root length

fresh shoot weight

and under ground fresh weight of millet were all reached. The maximum values were 15.46 cm

7.34 cm

0.64 g

and 0.16 g

respectively. As the concentration gradient of As increased

the fresh weight of the under ground layer increased first and then decreased

and the promotion and inhibition of the root system was greater than that of the shoot. The content of As in the roots of millet seedlings was significantly higher than in the shoots

and the TF value gradually dccreased. When As concentration was less than 4 mg/kg

As could promote the synthesis of chlorophyll in millet. When As concentration was 2 mg/kg

the total amount of chlorophyll a

chlorophyll b and chlorophyll in millet was significantly higher than that of the control 28.38%

51.60% and 37.06%

when the concentration of As was higher than 4 mg/kg

the chlorophyll synthesis of Millet was inhibited; with the increase of As concentration gradient

the SOD activity of millet seedlings continued to increase

and the activities of POD and CAT increased first and then decreased

but higher than the contro. When the As concentration exceeded 8 mg/kg

the POD activity of the millet seedlings began to decrease. After the As concentration exceeded 4 mg/kg

the CAT activity began to decrease. After As concentration higher than 8 mg/kg

the POD actibvity of millet seedlings began to decrease. The results showed that low concentration of As (<4 mg/kg) promoted the germination and seedling growth of millet

and the high concentration of As(≥ 4 mg/kg) inhibited the germination and seedling growth of millet

and the root system was more sensitive to As stress

and the activity of antioxidant enzyme system (POD

CAT) was reduced. The research can provide a scientific basis for the anti-pollution cultivation and pollution-free production of millet.

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