通过对湘东红壤丘陵区樟树(Cinnamomum camphora)人工林林下与林间土壤进行配对采样与分析,研究樟树人工林对退化红壤性质及微生物碳源利用的影响。结果表明:与林间土壤相比,林下土壤的pH增加0.95个单位,全磷、全钾和含水量分别提高14.8%,16.8%和45.6%,容重降低8.1%,均达到显著水平。樟树人工林显著增加林下土壤微生物的平均颜色变化率(average well color development,AWCD),林间土壤微生物对碳水化合物、氨基酸、羧酸、聚合物和酚酸类碳源的利用强度依次仅相当于林下土壤微生物的27.6%,19.2%,23.4%,47.1%和3.5%,但二者对胺类碳源的利用没有明显变化。樟树人工林显著增加林下土壤微生物的丰富度指数、多样性指数、优势度指数和均匀度指数。主成分分析发现,樟树人工林改变土壤微生物的碳源利用模式;相关性分析表明,樟林恢复后土壤理化性质的改善是造成微生物碳源利用能力和多样性指数提升的主要原因,而土壤pH是其中的首要驱动因素。综上,樟树人工林有助于提高湘东退化红壤的微生物生态功能,樟树是进行退化红壤生态修复和重建的适宜树种。
Abstract
In order to study the effects of Cinnamomum camphora plantations on properties of degraded red soil and utilization of microbial carbon sources in eastern Hu'nan
forest soil and non-forest soil samples were paired sampled and analyzed. The results showed that the pH in forest soil was significantly increased by 0.95 units compared with non-forest soil
and the total phosphorus
total potassium and water contents were significantly increased by 14.8%
16.8% and 45.6%
respectively. But the soil bulk density was significantly decreased by 8.1%. The average well color development (AWCD) of soil microorganisms was significantly increased by long-term Cinnamomum camphora restoration. Furthermore
the microbial metabolic intensities of carbohydrates
amino acids
carboxylic acids
polymers and phenolic compounds in non-forest soil were only 27.6%
19.2%
23.4%
47.1% and 3.5% of those in forest soil
respectively. In addition
soil microbial functional diversity indexes were significantly increased by long-term Cinnamomum camphora restoration. Principal component analysis (PCA) showed that Cinnamomum camphora plantations changed the carbon sources utilization pattern of soil microbial community. Correlation analysis showed that the improved soil physical and chemical properties after Cinnamomum camphora restoration were reasons for the improvement of microbial carbon sources utilization capacity and functional diversity indexes
and among which
soil pH was the primary driving factor. In conclusion
Cinnamomum camphora plantations could improve the microbial ecological function of degraded red soil in eastern Hu'nan
and Cinnamomum camphora is a suitable tree species for ecological restoration and reconstruction of degraded red soil.
Wu Z Y, Haack S E, Lin W X, et al. Soil microbial community structure and metabolic activity of
Pinuselliottii
plantations across different stand ages in a subtropical area[J].PloS One,2015,10(8):1-11.
Liu J, Liu M, Wu M, et al. Soil pH rather than nutrients drive changes in microbial community following long-term fertilization in acidic Ultisols of southern China[J].Journal of Soils and Sediments,2018,18(5):1853-1864.
Gomez E, Garland J L. Effects of tillage and fertilization on physiological profiles of soil microbial communities[J].Applied Soil Ecology,2012,61(5):327-332.
Rincon-Florez V A, Dang Y P, Crawford M H, et al. Occasional tillage has no effect on soil microbial biomass, activity and composition in Vertisols under long-term no-till[J].Biology and Fertility of Soils,2016,52:191-202.
Yin R, Deng H, Wang H L, et al. Vegetation type affects soil enzyme activities and microbial functional diversity following re-vegetation of a severely eroded red soil in sub-tropical China[J].Catena,2014,115(3):96-103.
Salles J F, Veen J A V, Elsas J D V. Multivariate analyses of Burkholderia species in soil:Effect of crop and land use history[J].Applied and Environmental Microbiology,2004,70(7):4012-4020.
Govaerts B, Mezzalama M, Unno Y, et al. Influence of tillage, residue management, and crop rotation on soil microbial biomass and catabolic diversity[J].Applied Soil Ecology,2007,37(1/2):18-30.
Zhong W H, Gu T, Wang W, et al. The effects of mineral fertilizer and organic manure on soil microbial community and diversity[J].Plant and Soil,2010,326:511-522.