Spatial Differentiation of Microorganisms in Profiles of Flooded Paddy Field in Southwest China

ZHANG Jian; SHEN Si; WAN Qihui; LI Ningning; JIANG Xianjun; LUO Hongyan

doi:10.13870/j.cnki.stbcxb.2018.06.045

您当前的位置：

首页 >

文章列表页 >

Spatial Differentiation of Microorganisms in Profiles of Flooded Paddy Field in Southwest China

更新时间：2025-03-12

- Spatial Differentiation of Microorganisms in Profiles of Flooded Paddy Field in Southwest China
- Vol. 32, Issue 6, Pages: 315-320(2018)
- 作者机构：
  
  西南大学资源环境学院,重庆,400715
- 作者简介：
- 基金信息：
- DOI：10.13870/j.cnki.stbcxb.2018.06.045
  CLC： S154.34
- Published：2018
- 稿件说明：
移动端阅览
ZHANG Jian, SHEN Si, WAN Qihui, et al. Spatial Differentiation of Microorganisms in Profiles of Flooded Paddy Field in Southwest China[J]. 2018, 32(6): 315-320.
DOI：

ZHANG Jian, SHEN Si, WAN Qihui, et al. Spatial Differentiation of Microorganisms in Profiles of Flooded Paddy Field in Southwest China[J]. 2018, 32(6): 315-320. DOI： 10.13870/j.cnki.stbcxb.2018.06.045.

摘要

为研究冬水田土壤基本理化性质对微生物多样性和冬水田生态系统功能与结构的作用，以西南地区3个不同样地的冬水田为研究对象，采用烘干法、电位法、静态室内培养法、稀释涂布平板法和氯仿熏蒸法对土壤理化和生物学指标进行检测。结果表明：（1）各样地含水量均沿垂直深度逐渐降低，pH为6.3~7.1，呈微酸—中性生境，铵态氮（NH4+—N）含量总体呈现为合川区冬水田 > 沙坪坝区冬水田 > 北碚区紫色土基地，且表层（0—10 cm） > 亚表层（10—20 cm） > 底层（20—40 cm），整体为36.97~52.02 mg/kg，3种冬水田各土层硝态氮（NO3-—N）含量差异不显著（2.13~2.61 mg/kg）。（2）微生物量碳（MBC）和微生物量氮（MBN）含量呈现为北碚区紫色土基地>合川区冬水田>沙坪坝区冬水田，不同层次表现为表层（0—10 cm）>亚表层（10—20 cm）>底层（20—40 cm）。（3）各样地间微生物丰度表现为细菌>放线菌>真菌，微生物数量沿土层垂直深度降低，各土层细菌、放线菌和真菌数量均呈极显著负相关（p<0.01），不同样地间表现为合川区冬水田>沙坪坝区冬水田>北碚区紫色土基地。

Abstract

In order to study the effect of the basic physicochemical properties on microbial diversity

three different flooded paddy in the Southwestern China were selected. Drying method

potential metric method

static indoor culture method

diluted coating plate method and chloroform fumigation method were used to test the soil physicochemical and biological indexes. The results indicated that:(1) The water content of each sample area gradually reduces along the vertical depth. The pH value was between 6.3~7.1 from slightly acidic to neutral. Ammonium nitrogen (NH4+-N) content ranged as Hechuan flooded paddy field>Shapingba flooded paddy field>Beibei purple soil base

and the surface (0-10 cm)>subsurface stratum (10-20 cm)>bottom (20-40 cm)

while the overall content was 36.97~52.02 mg/kg. The difference of nitrate nitrogen (NO3--N) in three kinds of flooded paddy fields was not significant (2.13~2.61 mg/kg). (2) The content of microbial biomass carbon (MBC)

microbial biomass nitrogen (MBN) in Beibei purple soil base was the most

followed by Hechuan flooded paddy field

and Shapingba flooded paddy field

while the content of MBC and MBN decreased with the increase of soil depth. (3) The microbial abundance showed the trend of bacteria>actinomycetes>fungi

and decreased with the increase of soil depth. The Pearson relationship analysis showed that the abundance of soil bacteria

actinomycetes and fungi had a significant negative relationship with each other (p<0.01). The microbial abundance showed the trend as Hechuan flooded paddy field>Shapingba flooded paddy field>Beibei purple soil base.

关键词

Keywords

references

Zhao H, Sun J, Xu X, et al. Stoichiometry of soil microbial biomass carbon and microbial biomass nitrogen in China's temperate and alpine grasslands[J].European Journal of Soil Biology,2017,83:1-8.

Siciliano S D, Palmer A S, Winsley T, et al. Soil fertility is associated with fungal andbacterial richness, where as pH is associated with community composition in polar soil microbial communities[J].Soil Biology and Biochemistry,2014,78(6):10-20.

李贵才,韩兴国,黄建辉,等.森林生态系统土壤氮矿化影响因素研究进展[J].生态学报,2001,21(7):1187-1195.

孙志高,刘景双,姜艳艳,等.基于地统计学和

N技术的湿地土壤氮素空间运移理论探讨[J].中国农学通报,2005,21(6):347-351.

Jenkinson D S, Brookes P C, Powlson D S. Measuring soil microbial biomass[J].Soil Biology and Biotechemistry,2004,36(1):5-7.

曹慧,杨浩,孙波,等.不同种植时间菜园土壤微生物生物量和酶活性变化特征[J].土壤,2002,34(4):197-200.

Ren C, Zhang W, Zhong Z, et al. Differential responses of soil microbial biomass, diversity, and compositions to altitudinal gradients depend on plant and soil characteristics[J].Science of the Total Environment,2017,610/611:750-758.

陈桂权.冬水田技术的形成与传播:以四川为中心的考察[J].中国农史,2013,32(4):3-13.

马秀梅,朱波,杜泽林,等.冬水田休闲期温室气体排放通量的研究[J].农业环境科学学报,2005,24(6):1199-1202.

孙波,潘贤章,王德建,等.我国不同区域农田养分平衡对土壤肥力时空演变的影响[J].地球科学进展,2008,23(11):1201-1208.

孙志高,刘景双,陈小兵.三江平原典型小叶章湿地土壤中硝态氮和铵态氮的空间分布格局[J].水土保持通报,2009,23(3):66-72.

刘光崧.土壤理化分析与剖面描述[M].北京:中国标准出版社,1996.

李阜棣.农业微生物学实验技术[M].北京:中国农业出版社,1996.

Classen A T, Overby S T S C, Koch G W, et al. Season mediates herbivore effects on litter and soil microbial andactivity in a semi-arid woodland[J].Plant and Soil,2007,295(1/2):217-227.

Breuer L, Huisman J A, Keller T, et al. Impact of a conversion from cropland to grassland on C and N storage and related soil properties:Analysis of a 60-year chronosequence[J].Geoderma,2006,133(1):6-18.

何振立.土壤微生物量及其在养分循环和环境质量评价中的意义[J].土壤,1997,29(2):61-69.

蔡昆争,骆世明,段舜山.水稻根系的空间分布及其与产量的关系[J].华南农业大学学报,2003,24(3):1-4.

赵先丽,程海涛,吕国红,等.土壤微生物生物量研究进展[J].气象与环境学报,2006,22(4):68-72.

Mistch W J, Gosselin J G.Wetlands[M].New York:Van Nostrand Reinhold Company Inc.,2000:89-125.

秦松,樊燕,刘洪斌,等.地形因子与土壤养分空间分布的相关性研究[J].水土保持研究,2008,15(1):275-279.

杜峰,梁宗锁,徐学选,等.陕北黄土丘陵区撂荒草地群落生物量及植被土壤养分效应[J].生态学报,2007,27(5):1673-1683.

陈效民,吴华山,孙静红.太湖地区农田土壤中铵态氮和硝态氮的时空变异[J].环境科学,2006,27(6):1217-1222.

张伟,陈洪松,王克林,等.桂西北喀斯特洼地土壤有机碳和速效磷的空间变异[J].生态学报,2007,27(12):5168-5175.

刘芳,樊小林,李天安,等.覆盖旱种水稻稻田土壤剖面硝态氮和铵态氮的动态变化[C]//中国土壤学会.第四届中国青年植物营养与肥料科学工作者学术讨论会论文集,2004.

任佐华,张于光,李迪强,等.三江源地区高寒草原土壤微生物活性和微生物量[J].生态学报,2011,31(11):3232-3238.

秦晓波,李玉娥,石生伟,等.稻田温室气体排放与土壤微生物菌群的多元回归分析[J].生态学报,2012,32(6):1811-1819.

李香兰,马静,徐华,等.水分管理对水稻生长期CH4和N2O排放季节变化的影响[J].农业环境科学学报,2008,27(2):535-541.

Bateman E J, Baggs E M. Contributions of nitrification and denitrification to N

O emissions from soils at different water-filled pore space[J].Biology and Fertility of Soils,2005,41(6):379-388.

Ghosh S, Majumdar D, Jain M C. Methane and nitrous oxide emissions from an irrigated rice of North India[J].Chemosphere,2003,51(3):181-195.

Views

1426

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of Continuous Returning Straw to Maize Tilth Soil on Chemical Character and Microbial Biomass

Difference in Absorbability of Different Aquatic Plants on N and P in Surface Water and Its Mechanism

Effects of Biochar Addtion on Physicochemical Properties and Microbial Biomass of the Red Paddy Soil Polluted by Heavy Metals

Effects of Different Straw Returning Methods on Soil Microbial Biomass Carbon, Nitrogen, Phosphorus and Soluble Organic Matter in Purple Soil

Related Author

WANG Han-ning

ZHANG En-he

张恩和

MU Ping

SONG Haixing

YAN Hongxing

ZHANG Yuping

XIE Guixian

Related Institution

MU Ping1,2,ZHANG En-he2,WANG Han-ning2,FANG Yong-feng2(1.Gansu Provincial Key Laboratory of Aridland Crop Sciences,Lanzhou 730070,2.College of Agronomy,Gansu Agricultural University,)

甘肃农业大学农学院

College of Resources and Environmental Sciences, Hunan Agricultural University, Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China, National Engineering Laboratory of Soil and Fertilizer Resources Efficient Utilization, Hunan Provincial Key Laboratory of Farmland Pollution Control and Agricultural Resources Use, Hunan Provincial Key Laboratory of Plant Nutrition in Common University

College of Resources and Environmental Science, Nanjing Agricultural University

College of Resources and Environment， Southwest University

⁰