短期氮添加对荒漠草原土壤微生物特征的影响

宋珂辰; 王星; 许冬梅; 李永康; 撒春宁; 马霜

doi:10.13870/j.cnki.stbcxb.2022.03.043

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短期氮添加对荒漠草原土壤微生物特征的影响

更新时间：2026-04-09

- 短期氮添加对荒漠草原土壤微生物特征的影响
- Effects of Short-term Nitrogen Addition on Soil Biological Properties in Desert Steppe
- 水土保持学报 2022年36卷第3期页码：303-310
- 作者机构：
  
  1. 宁夏大学农学院,银川,750021
  2. 宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,银川,750021
- 作者简介：
  
  [ "宋珂辰(1997-),女,硕士,主要从事草地生态、资源与环境研究。E-mail:457975218@qq.com" ]
- 基金信息：
  
  宁夏自治区重点研发计划一般项目(2018BEG03007);宁夏高等学校一流学科建设(草学学科)项目(NXYLXK2017A01)
- DOI：10.13870/j.cnki.stbcxb.2022.03.043
  中图分类号： S154.1
- 网络首发：2022-05-27，
  
  纸质出版：2022
- 稿件说明：
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宋珂辰, 王星, 许冬梅, 等. 短期氮添加对荒漠草原土壤微生物特征的影响[J]. 水土保持学报, 2022,36(3):303-310.

SONG Kechen, WANG Xing, XU Dongmei, et al. Effects of Short-term Nitrogen Addition on Soil Biological Properties in Desert Steppe[J]. 2022, 36(3): 303-310.
宋珂辰, 王星, 许冬梅, 等. 短期氮添加对荒漠草原土壤微生物特征的影响[J]. 水土保持学报, 2022,36(3):303-310. DOI： 10.13870/j.cnki.stbcxb.2022.03.043.

SONG Kechen, WANG Xing, XU Dongmei, et al. Effects of Short-term Nitrogen Addition on Soil Biological Properties in Desert Steppe[J]. 2022, 36(3): 303-310. DOI： 10.13870/j.cnki.stbcxb.2022.03.043.

摘要

为探究荒漠草原土壤微生物特征对短期氮添加的响应

以宁夏荒漠草原为对象

参照国内外同类研究和当地的氮沉降量

设置N0、N1、N2、N3、N4的5个处理

其纯氮添加量分别为0

2.5

15 g/(m2·a)

以尿素为氮源进行为期2年的氮添加试验

对不同氮添加处理下荒漠草原浅层土壤微生物特征进行了研究。结果表明:(1)随氮添加量的增加

荒漠草原0—20 cm土层土壤铵态氮含量呈升高的变化趋势

硝态氮含量呈先升高后下降的变化趋势。在0—10 cm土层

N1、N2、N3、N4处理草地铵态氮含量较N0处理分别增加了109.61％

136.52％

197.19％

198.88％

硝态氮含量以N2处理草地最高

显著高于N0、N3、N4处理(P<0.05)。(2)与N0相比

施氮后0—10 cm土层土壤微生物量氮含量显著下降

N1、N2、N3和N4处理分别较N0处理降低了37.54％

38.11％

28.56％

29.81％。(3)随氮添加量的增加

荒漠草原0—10 cm土层土壤真菌数量呈逐渐减少的变化趋势

N0、N1处理草地显著大于N3、N4处理草地(P＜0.05)。PcoA分析显示高氮添加处理(N3、N4)对荒漠草原土壤amoA区、nirK区微生物的群落结构有显著改变。高氮添加(N3、N4)会对荒漠草原土壤微生物群落产生负面影响

具体表现为真菌数量减少

硝态氮含量降低

氮转化微生物的α多样性和优势菌丰度降低

群落结构发生显著改变。

Abstract

Desert steppe belongs to the arid type of grassland

with low nutrient content

fragile ecological environment and sensitive response to disturbance. The gradual increase of global nitrogen deposition changed the process of carbon and nitrogen transformation in the ecosystem and affected soil properties. In order to explore the effects of nitrogen addition on biological properties of soil in desert steppe

we took the desert steppe in Yanchi County as the research object and set up 5 nitrogen treatments as N0

N3 and N4.These treatments were set based on the pure nitrogenaddition levels of 0

2.5

10 and 15 g/(m2·a)

by applying with urea as nitrogen source. Results showed that:(1) The NH4+-N content in 0-20 cm soil layer of desert steppe increased with the increase of nitrogen application rate

while the NO3--N content increased firstly and then decreased. In 0-10 cm soil layer

compared with N0

the NH4+-N contents under N1

N3 and N4 treatments were increased by 109.61%

136.52%

197.19% and 198.88%

respectively. The NO3--N content was the highest in N2 treatment

which was significantly higher than those in N0

N3 and N4 treatments (P<0.05). (2) Compared with N0

the MBN contents in 0-10 cm soil layer were significantly decreased by N1

N3 and N4 treatments

which were reduced by 37.54%

38.11%

28.56% and 29.81%

respectively. (3) With the increase of nitrogen addition

the number of fungi in 0-10 cm soil layer of desert steppe gradually decreased

among treatments

the N0 and N1 were significantly higher than N3 and N4 (P<0.05). PCoA analysis showed that the microbial community structure in amoA and nirK zones of desert steppe soil were significantly changed by high nitrogen addition. Based on this research

the high nitrogen addition (N3

N4) had negative effects on soil microbial community in desert steppe

which were as follows:the number of fungi decreased

NO3--N content decreased

α diversity and dominant bacteria abundance of nitrogen-transforming microorganisms decreased

and the microbial community structure of nitrogen-transforming changed significantly.

关键词

Keywords

references

Lamarque J F. Assessing future nitrogen deposition and carbon cycle feedback using a multimodel approach:Analysis of nitrogen deposition[J].Journal of Geophysical Research Atmospheres,2005,110(19):148-227.

Vitousek P, Aber J, Howarth R, et al. Human alteration of the global nitrogen cycle:Sources and Consequences[J].Ecological Applications,1997,7(3):737-750.

Griffiths B S, Ritz K, Bardgett R D, et al. Ecosystem response of pasture soil communities to fumigation induced microbial diversity reductions:An examination of the biodiversity-ecosystem function relationship[J].Oikos,2000,90(2):279-294.

王志瑞,杨山,马锐骜,等.内蒙古草甸草原土壤理化性质和微生物学特性对刈割与氮添加的响应[J].应用生态学报,2019,30(9):3010-3018.

Zhao Q, Zeng D H. Nitrogen addition effects on tree growth and soil properties mediated by soil phosphorus availability and tree mediated by soil phosphorus availablity and tree species identity[J].Forest Ecology and Management,2019,449:e117478.

Zhang T A, Chen H Y H, Ruan H H. Global negative effects of nitrogen deposition on soil microbes[J].The ISME Journal,2018,12(7):1817-1825.

Ke X B, Angel R, Lu Y H, et al. Niche differentiation of ammonia oxidizers and nitrite oxidizers in rice paddy soil[J].Environmental Microbiology,2013,15(8):2275-2292.

Shi X Z, Hu H W, Wang J Q, et al. Niche separation of comammox Nitrospira and canonical ammonia oxidizers in an acidic subtropical forest soil under long-term nitrogen deposition[J].Soil Biology and Biochemistry,2018,126:114-122.

刘红梅,张海芳,秦洁,等.模拟氮沉降对贝加尔针茅草原土壤氮转化微生物的影响[J].农业环境科学学报,2019,38(10):386-2394.

薛璟花,莫江明,李炯,等.氮沉降增加对土壤微生物的影响[J].生态环境,2005,14(5):777-782.

贺佩,李悦,江明兢,等.连续氮添加14年对温带典型草原土壤碳氮组分及物理结构的影响[J].生态学报,2021,41(5):1808-1823.

顾峰雪,黄玫,张远东,等.1961-2010年中国区域氮沉降时空格局模拟研究[J].生态学报,2016,36(12):3591-3600.

鲍士旦.土壤农化分析[M].北京:中国农业出版社,2000.

Zhou Z H, Wang C K, Zheng M H, et al. Patterns and mechanisms of responses by soil microbial communities to nitrogen addition[J].Soil Biology and Biochemistry,2017,115:433-441.

刘红梅,杨殿林,张海芳,等.氮添加对贝加尔针茅草原土壤细菌群落结构的影响[J].草业学报,2019,28(9):23-32.

王丽娜,罗久富,杨梅香,等.氮添加对退化高寒草地土壤微生物量碳氮的影响[J].草业学报,2019,28(7):38-48.

李素新,覃志杰,刘泰瑞,等.模拟氮沉降对华北落叶松人工林土壤微生物碳和微生物氮的动态影响[J].水土保持学报,2020,34(1):268-274.

刘红梅,张爱林,皇甫超河,等.氮沉降增加对贝加尔针茅草原土壤微生物群落结构的影响[J].生态环境学报,2017,26(7):1100-1106.

陈向峰,刘娟,姜培坤,等.模拟氮沉降对毛竹林土壤生化特性和酶活性的影响[J].水土保持学报,2020,34(5):277-28.

Huang J Y, Xu Y X, Yu H L, et al. Soil prokaryotic community shows no response to two years of simulated nitrogen deposition in an arid ecosystem in northwestern China[J].Environmental Microbiology,2020,23(2):1222-1237.

廖圣祥,任运涛,袁晓波,等.围封对黄土高原草地铵态氮和硝态氮的影响[J].草业科学,2016,33(6):1044-1053.

pastures[J].World Journal of Microbiology and Biotechnology,2014,30(1):99-107.

Cong J, Liu X D, Lu H, et al. Analyses of the inuencing factors of soil microbial functional gene diversity in tropical rainforest based on GeoChip 5.0[J].Genomics Data,2015(5):397-398.

Mirza B S, Potisap C, Nüsslein K, et al. Response of free-living nitrogen-fixing microorganisms to land use change in the Amazon rainforest[J].Applied and Environmental Microbiology,2014,80(1):281-288.

刘红梅,张海芳,皇甫超河,等.长期氮添加对贝加尔针茅草原土壤微生物群落多样性的影响[J].农业环境科学学报,2017,36(4):709-717.

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